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Protein

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein

Gene

Prex2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGuanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-MMU-8948751 Regulation of PTEN stability and activity

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein
Short name:
P-Rex2
Short name:
PtdIns(3,4,5)-dependent Rac exchanger 2
Alternative name(s):
DEP domain-containing protein 2
Gene namesi
Name:Prex2
Synonyms:Depdc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1923385 Prex2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002867961 – 1598Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 proteinAdd BLAST1598

Proteomic databases

MaxQBiQ3LAC4
PaxDbiQ3LAC4
PeptideAtlasiQ3LAC4
PRIDEiQ3LAC4

PTM databases

iPTMnetiQ3LAC4
PhosphoSitePlusiQ3LAC4

Expressioni

Gene expression databases

BgeeiENSMUSG00000048960 Expressed in 210 organ(s), highest expression level in lung
ExpressionAtlasiQ3LAC4 baseline and differential
GenevisibleiQ3LAC4 MM

Interactioni

Subunit structurei

Interacts with RAC1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi224637, 1 interactor
STRINGi10090.ENSMUSP00000027056

Structurei

3D structure databases

ProteinModelPortaliQ3LAC4
SMRiQ3LAC4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 206DHPROSITE-ProRule annotationAdd BLAST192
Domaini237 – 353PHPROSITE-ProRule annotationAdd BLAST117
Domaini382 – 456DEP 1PROSITE-ProRule annotationAdd BLAST75
Domaini483 – 558DEP 2PROSITE-ProRule annotationAdd BLAST76
Domaini584 – 663PDZ 1PROSITE-ProRule annotationAdd BLAST80
Domaini669 – 746PDZ 2PROSITE-ProRule annotationAdd BLAST78

Domaini

PH domain confers substrate specificity and recognition. Able to discriminate between RAC1, RHOA, and CDC42 (By similarity).By similarity
DH domain alone was unable to confer substrate specificity and recognition.By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3519 Eukaryota
KOG4428 Eukaryota
ENOG410XRJD LUCA
GeneTreeiENSGT00930000150835
HOGENOMiHOG000115690
HOVERGENiHBG053677
InParanoidiQ3LAC4
KOiK17588
OMAiIDKLMRP
OrthoDBiEOG091G00MJ
TreeFamiTF328639

Family and domain databases

CDDicd04439 DEP_1_P-Rex, 1 hit
cd00160 RhoGEF, 1 hit
Gene3Di1.10.10.10, 2 hits
1.20.900.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR035899 DBL_dom_sf
IPR000591 DEP_dom
IPR000219 DH-domain
IPR001331 GDS_CDC24_CS
IPR001478 PDZ
IPR036034 PDZ_sf
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR037367 Rex2_DEP_1
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00610 DEP, 2 hits
PF00169 PH, 1 hit
PF00621 RhoGEF, 1 hit
SMARTiView protein in SMART
SM00049 DEP, 2 hits
SM00228 PDZ, 2 hits
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SUPFAMiSSF46785 SSF46785, 2 hits
SSF48065 SSF48065, 1 hit
SSF50156 SSF50156, 2 hits
PROSITEiView protein in PROSITE
PS50186 DEP, 2 hits
PS00741 DH_1, 1 hit
PS50010 DH_2, 1 hit
PS50106 PDZ, 2 hits
PS50003 PH_DOMAIN, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q3LAC4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSDESAREVD KQLRLRVCVL SELQKTERDY VGTLEFLVSA FLHRMNQCAA
60 70 80 90 100
AKVDKNVTEE TVKMLFSNIE EILIVHKEFL KVVEECLYPE PSAQQEVGAC
110 120 130 140 150
FLHFKDKFRI YDEYCSNHEK AQKLLLELNK IRTIRTFLLN CMLLGGRKNT
160 170 180 190 200
DVPLEGYLVT PIQRICKYPL LLKELLKRTP RRHSDYTAVM EALQAMKAVC
210 220 230 240 250
SNINEAKRQM EKLEVLEEWQ AHIEGWEGSN ITDTCTEMLM CGVLMKISSG
260 270 280 290 300
NIQERVFFLF DNLLVYCKRK HRRLKNSKAS TDGYRYVFRG RINTEVMEVE
310 320 330 340 350
NVDDGTADFH SSGHIVVNGW KIHNTAKNKW FVCMAKSPEE KHEWFEAILK
360 370 380 390 400
ERERRKGLKL GMEQDTWVMI SEQGEKLYKM MCKQGNLIKD RKRKLTTFPK
410 420 430 440 450
CFLGSEFVSW LLEIGEIHRP EEGVHLGQAL LENGIIHHVT DKHQFKPEQM
460 470 480 490 500
LYRFRYDDGT FYPRSEMQDV ISKGVRLYCR LHSLFTPVVR DKDYHLRTYK
510 520 530 540 550
SVVMANKLID WLIAQGDCRT REEAMIFAVG LCDNGFMHHV LEKSEFKDEP
560 570 580 590 600
LLFRFFADEE MEGSNMKHRL MKHDLKVVEN VIAKSLLIKS NEGSYGFGLE
610 620 630 640 650
DKNKVPIIKL VEKGSNAEMA GMEVGKKIFA INGDLVFLRP FPEVDCFLKS
660 670 680 690 700
CLNSRKPLRV LVSTKPRETV KIPDSADGLG FQIRGFGPSV VHAVGRGTVA
710 720 730 740 750
AAAGLHPGQC IIKVNGINVS KETHASVIAH VTACRKYKRP MKQDSIQWVY
760 770 780 790 800
DSLESAQEDI QKSHSKPPGD GAGDAFECKV EDVIDKFNTM AIIDGKKEHV
810 820 830 840 850
SLTVDNVHLE YGVVYEYDST AGTKCNVVEK MVEPKGFFSL TAKILEALAK
860 870 880 890 900
SDEHFVQNCT SLNSLNEVIA TDLQSKFTSM CSERIEHVCH RISSYGRFSR
910 920 930 940 950
VLKNRAWPTF KQAKPKISPL HSSDFCPTNC HVNVMEVSYP KTSTSLGSAF
960 970 980 990 1000
GVQLDSRKHN SHDKENKSVE PGKLSPMVYI QHTITTMAAP SGLSLGHKDG
1010 1020 1030 1040 1050
HGLQYLLKEE DLETQDIYHK LLGKLQTALK EVEMSVCQID DLLSSITYSP
1060 1070 1080 1090 1100
KLERKTTECV TPMDSDNEKG ERNSKRVCFN VAGDEQEDSG HDTVSNRDSY
1110 1120 1130 1140 1150
SDCNSNRNSI ASFTSICSSQ CSSYFHSDEM DSGDELPISV RISHDKQDKI
1160 1170 1180 1190 1200
HTCLEQLFSQ IDSIINLLKG QAVIRAFEQT KYLTPGRGLQ EFQQEMEAKL
1210 1220 1230 1240 1250
SCPRRLRLHL KQDPWNLPSS IQALAQSIRK HAEEVKCRIL LALLEYSDSE
1260 1270 1280 1290 1300
TQLRRDMVFC QSLVATVCAF SEQLMAALNQ MFDNSKENEM ETCEASRRWL
1310 1320 1330 1340 1350
DQIANAGVLF HFQSLLSPNL KDEQAMLEDT LVALFDLEKV SFFFKPSEED
1360 1370 1380 1390 1400
PLVANVPLTY QVEGSRQALK VYFYMDSYHF EQLPQRLKNG GGFKIHPVLF
1410 1420 1430 1440 1450
SQALESMEGY CYRDNISVEE FQAQINTASL EKVKQYNQKL RAFYLDKSNS
1460 1470 1480 1490 1500
PPNTTSKAAY IDKLMKPLNA LDELYRLITS FIRSKRIAAC VNTPCSASGV
1510 1520 1530 1540 1550
GLLSVSSELC DRLGACHIIM CSSGVHRCTL SVTLEQTITL ARSHGLPPRY
1560 1570 1580 1590
IMQAMDVMRK QGARVQNTAK NLGVRDRTPQ SAPRLYKLCE PPPPVGEE
Length:1,598
Mass (Da):181,717
Last modified:July 27, 2011 - v2
Checksum:iAC9507BC9CC5EAD6
GO
Isoform 2 (identifier: Q3LAC4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     698-734: TVAAAAGLHPGQCIIKVNGINVSKETHASVIAHVTAC → ETFFAAASRSAPAGPLALPCPTEGSPQSARNGPLSLK
     735-1598: Missing.

Note: No experimental confirmation available.
Show »
Length:734
Mass (Da):84,364
Checksum:iCE509293E946F278
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WRD8A0A087WRD8_MOUSE
Phosphatidylinositol 3,4,5-trisphos...
Prex2
40Annotation score:
A0A087WRG8A0A087WRG8_MOUSE
Phosphatidylinositol 3,4,5-trisphos...
Prex2
15Annotation score:
A0A087WS21A0A087WS21_MOUSE
Phosphatidylinositol 3,4,5-trisphos...
Prex2
24Annotation score:
A0A087WRV5A0A087WRV5_MOUSE
Phosphatidylinositol 3,4,5-trisphos...
Prex2
50Annotation score:

Sequence cautioni

The sequence BAB31066 differs from that shown. Reason: Frameshift at positions 498 and 500.Curated
The sequence BAE25059 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1166N → T in CAJ33348 (PubMed:15304343).Curated1
Sequence conflicti1176 – 1178AFE → PFD in CAJ33348 (PubMed:15304343).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_025164698 – 734TVAAA…HVTAC → ETFFAAASRSAPAGPLALPC PTEGSPQSARNGPLSLK in isoform 2. 1 PublicationAdd BLAST37
Alternative sequenceiVSP_025165735 – 1598Missing in isoform 2. 1 PublicationAdd BLAST864

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM109952 mRNA Translation: CAJ33348.1
AC102481 Genomic DNA No translation available.
AC102529 Genomic DNA No translation available.
AC102631 Genomic DNA No translation available.
AC164600 Genomic DNA No translation available.
AK018093 mRNA Translation: BAB31066.1 Sequence problems.
AK138884 mRNA Translation: BAE23811.1
AK142414 mRNA Translation: BAE25059.1 Different initiation.
CCDSiCCDS48217.1 [Q3LAC4-1]
RefSeqiNP_083801.1, NM_029525.1 [Q3LAC4-1]
UniGeneiMm.123247

Genome annotation databases

EnsembliENSMUST00000027056; ENSMUSP00000027056; ENSMUSG00000048960 [Q3LAC4-1]
GeneIDi109294
KEGGimmu:109294
UCSCiuc007ahs.2 mouse [Q3LAC4-1]
uc007aht.1 mouse [Q3LAC4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM109952 mRNA Translation: CAJ33348.1
AC102481 Genomic DNA No translation available.
AC102529 Genomic DNA No translation available.
AC102631 Genomic DNA No translation available.
AC164600 Genomic DNA No translation available.
AK018093 mRNA Translation: BAB31066.1 Sequence problems.
AK138884 mRNA Translation: BAE23811.1
AK142414 mRNA Translation: BAE25059.1 Different initiation.
CCDSiCCDS48217.1 [Q3LAC4-1]
RefSeqiNP_083801.1, NM_029525.1 [Q3LAC4-1]
UniGeneiMm.123247

3D structure databases

ProteinModelPortaliQ3LAC4
SMRiQ3LAC4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi224637, 1 interactor
STRINGi10090.ENSMUSP00000027056

PTM databases

iPTMnetiQ3LAC4
PhosphoSitePlusiQ3LAC4

Proteomic databases

MaxQBiQ3LAC4
PaxDbiQ3LAC4
PeptideAtlasiQ3LAC4
PRIDEiQ3LAC4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027056; ENSMUSP00000027056; ENSMUSG00000048960 [Q3LAC4-1]
GeneIDi109294
KEGGimmu:109294
UCSCiuc007ahs.2 mouse [Q3LAC4-1]
uc007aht.1 mouse [Q3LAC4-2]

Organism-specific databases

CTDi80243
MGIiMGI:1923385 Prex2

Phylogenomic databases

eggNOGiKOG3519 Eukaryota
KOG4428 Eukaryota
ENOG410XRJD LUCA
GeneTreeiENSGT00930000150835
HOGENOMiHOG000115690
HOVERGENiHBG053677
InParanoidiQ3LAC4
KOiK17588
OMAiIDKLMRP
OrthoDBiEOG091G00MJ
TreeFamiTF328639

Enzyme and pathway databases

ReactomeiR-MMU-8948751 Regulation of PTEN stability and activity

Miscellaneous databases

PROiPR:Q3LAC4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000048960 Expressed in 210 organ(s), highest expression level in lung
ExpressionAtlasiQ3LAC4 baseline and differential
GenevisibleiQ3LAC4 MM

Family and domain databases

CDDicd04439 DEP_1_P-Rex, 1 hit
cd00160 RhoGEF, 1 hit
Gene3Di1.10.10.10, 2 hits
1.20.900.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR035899 DBL_dom_sf
IPR000591 DEP_dom
IPR000219 DH-domain
IPR001331 GDS_CDC24_CS
IPR001478 PDZ
IPR036034 PDZ_sf
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR037367 Rex2_DEP_1
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00610 DEP, 2 hits
PF00169 PH, 1 hit
PF00621 RhoGEF, 1 hit
SMARTiView protein in SMART
SM00049 DEP, 2 hits
SM00228 PDZ, 2 hits
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SUPFAMiSSF46785 SSF46785, 2 hits
SSF48065 SSF48065, 1 hit
SSF50156 SSF50156, 2 hits
PROSITEiView protein in PROSITE
PS50186 DEP, 2 hits
PS00741 DH_1, 1 hit
PS50010 DH_2, 1 hit
PS50106 PDZ, 2 hits
PS50003 PH_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPREX2_MOUSE
AccessioniPrimary (citable) accession number: Q3LAC4
Secondary accession number(s): E9QMG3
, Q3UQI2, Q3UU18, Q9CXD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 107 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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