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Protein

Ferric reduction oxidase 7, chloroplastic

Gene

FRO7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Ferric chelate reductase involved in iron mobilization from the cytosol into the chloroplast. May participate in the transport of electrons to a Fe3+ ion via FAD and heme intermediates. Might be involved iron homeostasis in trichomes.2 Publications

Catalytic activityi

2 Fe(II)-siderophore + NAD+ + H+ = 2 Fe(III)-siderophore + NADH.

Cofactori

FADCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi223Iron (heme axial ligand)By similarity1
Metal bindingi237Iron (heme axial ligand)By similarity1
Metal bindingi298Iron (heme axial ligand)By similarity1
Metal bindingi311Iron (heme axial ligand)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi386 – 389FADSequence analysis4
Nucleotide bindingi446 – 449NADSequence analysis4

GO - Molecular functioni

  • ferric-chelate reductase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • ion transport Source: UniProtKB-KW
  • photosynthetic electron transport chain Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Ion transport, Transport
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, NAD

Enzyme and pathway databases

BioCyciARA:AT5G49740-MONOMER
MetaCyc:AT5G49740-MONOMER
BRENDAi1.16.1.7 399
ReactomeiR-ATH-3299685 Detoxification of Reactive Oxygen Species

Names & Taxonomyi

Protein namesi
Recommended name:
Ferric reduction oxidase 7, chloroplastic (EC:1.16.1.7)
Short name:
AtFRO7
Alternative name(s):
Ferric-chelate reductase 7
Gene namesi
Name:FRO7
Ordered Locus Names:At5g49740
ORF Names:K2I5.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G49740
TAIRilocus:2157032 AT5G49740

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei37 – 56HelicalBy similarityAdd BLAST20
Transmembranei81 – 99HelicalBy similarityAdd BLAST19
Transmembranei132 – 155HelicalBy similarityAdd BLAST24
Transmembranei222 – 245HelicalBy similarityAdd BLAST24
Transmembranei297 – 321HelicalBy similarityAdd BLAST25
Transmembranei344 – 364HelicalBy similarityAdd BLAST21
Transmembranei574 – 596HelicalBy similarityAdd BLAST23
Transmembranei612 – 634HelicalBy similarityAdd BLAST23

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype when grown under normal conditions. Reduced photosynthetic electron transport efficiency, chloroplast ferric chelate reductase activity and iron content. Seedling lethal when grown in alkaline soil.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 28ChloroplastSequence analysisAdd BLAST28
ChainiPRO_000041320529 – 747Ferric reduction oxidase 7, chloroplasticAdd BLAST719

Proteomic databases

PaxDbiQ3KTM0

Expressioni

Tissue specificityi

Expressed in shoots, flowers and siliques. Detected in cotyledons, leaves and trichomes, but not in roots.3 Publications

Developmental stagei

Expressed during cell or organ differentiation.1 Publication

Inductioni

Up-regulated in shoots by iron deficiency and down-regulated in shoots by copper deficiency.1 Publication

Gene expression databases

ExpressionAtlasiQ3KTM0 baseline and differential
GenevisibleiQ3KTM0 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G49740.1

Structurei

3D structure databases

ProteinModelPortaliQ3KTM0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini187 – 308Ferric oxidoreductaseAdd BLAST122
Domaini337 – 454FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST118

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi10 – 30Ser-richAdd BLAST21

Sequence similaritiesi

Belongs to the ferric reductase (FRE) family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039 Eukaryota
ENOG410XNZY LUCA
HOGENOMiHOG000006154
InParanoidiQ3KTM0
KOiK00521
OMAiVDAQFIS
OrthoDBiEOG093603NP
PhylomeDBiQ3KTM0

Family and domain databases

Gene3Di3.40.50.80, 2 hits
InterProiView protein in InterPro
IPR000778 Cyt_b245_heavy_chain
IPR013112 FAD-bd_8
IPR017927 Fd_Rdtase_FAD-bd
IPR013130 Fe3_Rdtase_TM_dom
IPR013121 Fe_red_NAD-bd_6
IPR039261 FNR_nucleotide_bd
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF08022 FAD_binding_8, 1 hit
PF01794 Ferric_reduct, 1 hit
PF08030 NAD_binding_6, 1 hit
PRINTSiPR00466 GP91PHOX
SUPFAMiSSF52343 SSF52343, 2 hits
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3KTM0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDHETPLLS KDLSSSSSSS SSSSSVVVSS LKWILKVVMS VIFVTWVVFL
60 70 80 90 100
MMYPGSLGDQ ILTNWRAISS NTLFGLTGSM FLIFSGPILV IAILASLYLI
110 120 130 140 150
ISGEETVFTK KKITKFPRFR LWTFPVLVDG PFGVVSAAEF LGIMVFSVFF
160 170 180 190 200
LWAIYAYTLR NLNVLDYFHV LPNNRSIFLL ELTGLRFGMI GLLCMVFLFL
210 220 230 240 250
PISRGSILLR LIDIPFEHAT RYHVWLGHIT MTFFSLHGLC YVVGWTIQGQ
260 270 280 290 300
LLELLFEWKA TGIAVLPGVI SLVAGLLMWV TSLHTVRKNY FELFFYTHQL
310 320 330 340 350
YIVFVVFLAL HVGDYLFSIV AGGIFLFILD RFLRFYQSRR TVDVISAKSL
360 370 380 390 400
PCGTLELVLS KPPNMRYNAL SFIFLQVKEL SWLQWHPFSV SSSPLDGNHH
410 420 430 440 450
VAVLIKVLGG WTAKLRDQLS TLYEAENQDQ LISPESYPKI TTCVEGPYGH
460 470 480 490 500
ESPYHLAYEN LVLVAGGIGI TPFFAILSDI LHRKRDGKDC LPGKVLVVWA
510 520 530 540 550
IKNSDELSLL SAIDIPSICH FFSKKLNLEI HIYVTRQSEP CLEDGMVHKV
560 570 580 590 600
VHPSVKTPWT NGCSMSVLVG TGDNIWSGLY LIISTIGFIA MITLVDIFYI
610 620 630 640 650
NKYNITTWWY KGLLFVVCMV ASVLIFGGLV VVFWHRWEHK TGEVEANGND
660 670 680 690 700
KVDLNGEETH NPSAAELKGL AIEEDVQNYT TIRYGTRPAF REIFESLNGK
710 720 730 740
WGSVDVGVIV CGPATLQTTV AKEIRSHSIW RSANHPLFHF NSHSFDL
Length:747
Mass (Da):84,126
Last modified:November 8, 2005 - v1
Checksum:iBB09E91DDC5F5207
GO

Sequence cautioni

The sequence BAA98162 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY912280 mRNA Translation: AAX92640.1
AB025613 Genomic DNA Translation: BAA98162.1 Sequence problems.
CP002688 Genomic DNA Translation: AED95852.1
AK227087 mRNA Translation: BAE99139.1
RefSeqiNP_199785.2, NM_124352.4
UniGeneiAt.29770
At.7122

Genome annotation databases

EnsemblPlantsiAT5G49740.1; AT5G49740.1; AT5G49740
GeneIDi835037
GrameneiAT5G49740.1; AT5G49740.1; AT5G49740
KEGGiath:AT5G49740

Similar proteinsi

Entry informationi

Entry nameiFRO7_ARATH
AccessioniPrimary (citable) accession number: Q3KTM0
Secondary accession number(s): Q9LT99
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: November 8, 2005
Last modified: July 18, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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