UniProtKB - Q3KNA1 (MRGB2_MOUSE)
Protein
Mas-related G-protein coupled receptor member B2
Gene
Mrgprb2
Organism
Mus musculus (Mouse)
Status
Functioni
Mast cell-specific receptor for basic secretagogues, i.e. cationic amphiphilic drugs, as well as endo- or exogenous peptides, consisting of a basic head group and a hydrophobic core. Recognizes and binds small molecules containing a cyclized tetrahydroisoquinoline (THIQ), such as non-steroidal neuromuscular blocking drugs (NMBDs), including tubocurarine and atracurium. In response to these compounds, mediates pseudo-allergic reactions characterized by histamine release, inflammation and airway contraction (PubMed:25517090).1 Publication
Caution
In spite of its official gene name, this protein may be the functional ortholog of human MRGPRX2, in terms of expression pattern and pharmacology.1 Publication
GO - Molecular functioni
- G protein-coupled receptor activity Source: GO_Central
- transmembrane signaling receptor activity Source: MGI
GO - Biological processi
- cell surface receptor signaling pathway Source: MGI
- G protein-coupled receptor signaling pathway Source: GO_Central
- response to chloroquine Source: MGI
- signal transduction Source: MGI
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Names & Taxonomyi
Protein namesi | Recommended name: Mas-related G-protein coupled receptor member B2 |
Gene namesi | Name:Mrgprb2 Synonyms:Mrgb2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2441674 Mrgprb2 |
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Multi-pass membrane protein Curated
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 40 | ExtracellularSequence analysisAdd BLAST | 40 | |
Transmembranei | 41 – 61 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 62 – 89 | CytoplasmicSequence analysisAdd BLAST | 28 | |
Transmembranei | 90 – 110 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 111 | ExtracellularSequence analysis | 1 | |
Transmembranei | 112 – 132 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 133 – 157 | CytoplasmicSequence analysisAdd BLAST | 25 | |
Transmembranei | 158 – 178 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 179 – 191 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 192 – 212 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 213 – 231 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 232 – 252 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 253 – 268 | ExtracellularSequence analysisAdd BLAST | 16 | |
Transmembranei | 269 – 289 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 290 – 338 | CytoplasmicSequence analysisAdd BLAST | 49 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
No visible phenotype in normal conditions. Absence of pseudo-allergic reactions in response to small-molecule therapeutic drugs: secretagogue-induced histamine release, inflammation and airway contraction are abolished.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000304878 | 1 – 338 | Mas-related G-protein coupled receptor member B2Add BLAST | 338 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 9 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 18 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 23 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q3KNA1 |
PRIDEi | Q3KNA1 |
PTM databases
iPTMneti | Q3KNA1 |
PhosphoSitePlusi | Q3KNA1 |
Expressioni
Tissue specificityi
Mast cell-specific.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000050425 Expressed in 8 organ(s), highest expression level in zone of skin |
ExpressionAtlasi | Q3KNA1 baseline and differential |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG410J9NM Eukaryota ENOG4111AUB LUCA |
GeneTreei | ENSGT00940000153553 |
HOGENOMi | HOG000015412 |
HOVERGENi | HBG003592 |
InParanoidi | Q3KNA1 |
KOi | K08396 |
OMAi | NAIVLWF |
OrthoDBi | 1123658at2759 |
PhylomeDBi | Q3KNA1 |
TreeFami | TF336336 |
Family and domain databases
InterProi | View protein in InterPro IPR000276 GPCR_Rhodpsn IPR017452 GPCR_Rhodpsn_7TM IPR026234 MRGPCRFAMILY |
PANTHERi | PTHR11334 PTHR11334, 1 hit |
Pfami | View protein in Pfam PF00001 7tm_1, 1 hit |
PRINTSi | PR00237 GPCRRHODOPSN PR02108 MRGPCRFAMILY |
PROSITEi | View protein in PROSITE PS00237 G_PROTEIN_RECEP_F1_1, 1 hit PS50262 G_PROTEIN_RECEP_F1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q3KNA1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSGDFLIKNL STSAWKTNIT VLNGSYYIDT SVCVTRNQAM ILLSIIISLV
60 70 80 90 100
GMGLNAIVLW FLGIRMHTNA FTVYILNLAM ADFLYLCSQF VICLLIAFYI
110 120 130 140 150
FYSIDINIPL VLYVVPIFAY LSGLSILSTI SIERCLSVIW PIWYRCKRPR
160 170 180 190 200
HTSAITCFVL WVMSLLLGLL EGKACGLLFN SFDSYWCETF DVITNIWSVV
210 220 230 240 250
FFGVLCGSSL TLLVRIFCGS QRIPMTRLYV TITLTVLVFL IFGLPFGIYW
260 270 280 290 300
ILYQWISNFY YVEICNFYLE ILFLSCVNSC MNPIIYFLVG SIRHRRFRRK
310 320 330
TLKLLLQRAM QDTPEEEQSG NKSSSEHPEE LETVQSCS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 28 | I → F in BAC26422 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 263 | E → K in AAI07391 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 263 | E → K in AAI07392 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY042200 Genomic DNA Translation: AAK91796.1 AK029369 mRNA Translation: BAC26422.1 BC107390 mRNA Translation: AAI07391.1 BC107391 mRNA Translation: AAI07392.1 |
CCDSi | CCDS21303.1 |
RefSeqi | NP_780740.2, NM_175531.4 |
UniGenei | Mm.83689 |
Genome annotation databases
Ensembli | ENSMUST00000052730; ENSMUSP00000061878; ENSMUSG00000050425 |
GeneIDi | 243979 |
KEGGi | mmu:243979 |
UCSCi | uc009hat.2 mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY042200 Genomic DNA Translation: AAK91796.1 AK029369 mRNA Translation: BAC26422.1 BC107390 mRNA Translation: AAI07391.1 BC107391 mRNA Translation: AAI07392.1 |
CCDSi | CCDS21303.1 |
RefSeqi | NP_780740.2, NM_175531.4 |
UniGenei | Mm.83689 |
3D structure databases
ProteinModelPortali | Q3KNA1 |
ModBasei | Search... |
MobiDBi | Search... |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
iPTMneti | Q3KNA1 |
PhosphoSitePlusi | Q3KNA1 |
Proteomic databases
PaxDbi | Q3KNA1 |
PRIDEi | Q3KNA1 |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENSMUST00000052730; ENSMUSP00000061878; ENSMUSG00000050425 |
GeneIDi | 243979 |
KEGGi | mmu:243979 |
UCSCi | uc009hat.2 mouse |
Organism-specific databases
CTDi | 243979 |
MGIi | MGI:2441674 Mrgprb2 |
Phylogenomic databases
eggNOGi | ENOG410J9NM Eukaryota ENOG4111AUB LUCA |
GeneTreei | ENSGT00940000153553 |
HOGENOMi | HOG000015412 |
HOVERGENi | HBG003592 |
InParanoidi | Q3KNA1 |
KOi | K08396 |
OMAi | NAIVLWF |
OrthoDBi | 1123658at2759 |
PhylomeDBi | Q3KNA1 |
TreeFami | TF336336 |
Miscellaneous databases
PROi | PR:Q3KNA1 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000050425 Expressed in 8 organ(s), highest expression level in zone of skin |
ExpressionAtlasi | Q3KNA1 baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR000276 GPCR_Rhodpsn IPR017452 GPCR_Rhodpsn_7TM IPR026234 MRGPCRFAMILY |
PANTHERi | PTHR11334 PTHR11334, 1 hit |
Pfami | View protein in Pfam PF00001 7tm_1, 1 hit |
PRINTSi | PR00237 GPCRRHODOPSN PR02108 MRGPCRFAMILY |
PROSITEi | View protein in PROSITE PS00237 G_PROTEIN_RECEP_F1_1, 1 hit PS50262 G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | MRGB2_MOUSE | |
Accessioni | Q3KNA1Primary (citable) accession number: Q3KNA1 Secondary accession number(s): Q8CDY4, Q91ZC2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 11, 2007 |
Last sequence update: | September 11, 2007 | |
Last modified: | February 13, 2019 | |
This is version 95 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries