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Protein

tRNA sulfurtransferase

Gene

thiI

Organism
Pseudomonas fluorescens (strain Pf0-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

Catalytic activityi

ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster.UniRule annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei267ATPUniRule annotation1
Binding sitei289ATP; via amide nitrogenUniRule annotation1
Binding sitei298ATPUniRule annotation1
Active sitei457Cysteine persulfide intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi185 – 186ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Transferase, tRNA-binding
Biological processThiamine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
Thiamine biosynthesis protein ThiIUniRule annotation
tRNA 4-thiouridine synthaseUniRule annotation
Gene namesi
Name:thiIUniRule annotation
Ordered Locus Names:Pfl01_0346
OrganismiPseudomonas fluorescens (strain Pf0-1)
Taxonomic identifieri205922 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002704 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000742531 – 484tRNA sulfurtransferaseAdd BLAST484

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi346 ↔ 457Redox-activeUniRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ3KJG6

Interactioni

Protein-protein interaction databases

STRINGi205922.Pfl01_0346

Structurei

3D structure databases

ProteinModelPortaliQ3KJG6
SMRiQ3KJG6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 167THUMPUniRule annotationAdd BLAST105
Domaini405 – 483RhodaneseUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the ThiI family.UniRule annotation

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiENOG4105D8I Bacteria
COG0301 LUCA
COG0607 LUCA
HOGENOMiHOG000227469
KOiK03151
OMAiKLFPEIM
OrthoDBiPOG091H04VG

Family and domain databases

CDDicd01712 ThiI, 1 hit
Gene3Di3.40.250.10, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00021 ThiI, 1 hit
InterProiView protein in InterPro
IPR001763 Rhodanese-like_dom
IPR036873 Rhodanese-like_dom_sf
IPR014729 Rossmann-like_a/b/a_fold
IPR026340 Thiazole_biosynth_dom
IPR020536 ThiI_AANH
IPR004114 THUMP_dom
IPR003720 tRNA_STrfase
PfamiView protein in Pfam
PF02568 ThiI, 1 hit
PF02926 THUMP, 1 hit
SMARTiView protein in SMART
SM00981 THUMP, 1 hit
SUPFAMiSSF52821 SSF52821, 1 hit
TIGRFAMsiTIGR04271 ThiI_C_thiazole, 1 hit
TIGR00342 TIGR00342, 1 hit
PROSITEiView protein in PROSITE
PS50206 RHODANESE_3, 1 hit
PS51165 THUMP, 1 hit

Sequencei

Sequence statusi: Complete.

Q3KJG6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLIVKVFPE ITIKSRPVRT KFIRQLAKNI RTVLRDLDPA VVVNGVWDNL
60 70 80 90 100
ELETRVTDPK ALKEMGERLT CMPGIAHFLQ IDEYPLGDFD DITEKCKQHY
110 120 130 140 150
GDALAGKIFS VRCKRAGKHA FSSMDVEKYV GSKLRRECGA AGIDLKQPEI
160 170 180 190 200
EVRIEVRDKR LFVIHSQHNG IGGYPLGALE QTLVLMSGGF DSTVAAYQIL
210 220 230 240 250
RRGLMTHFCF FNLGGRAHEL GVMEVAHFIW KKYGSSQRVL FVSVPFEEVL
260 270 280 290 300
GEILGKVDDS HMGVVLKRMM LRAASSIAER LHIDALVTGE AISQVSSQTL
310 320 330 340 350
PNLSVIDCVT DKLVLRPLIV AHKQDIIDTA DQIGTGDFAR HMPEYCGVIS
360 370 380 390 400
VNPKTAAKRG RVEHEEQEFD MAVLERALAN ARLVPIDRVI DELGQDLQIE
410 420 430 440 450
EVSEALAGQI VIDIRHPDAA EDDPLELAGI EVQTMPFYAV NARFKELDPT
460 470 480
RQYLLYCDKG VMSRLHAHHL LSEGHANVRV YRPS
Length:484
Mass (Da):54,193
Last modified:November 8, 2005 - v1
Checksum:iABD3F5C83DDE633C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000094 Genomic DNA Translation: ABA72090.1
RefSeqiWP_011332034.1, NC_007492.2

Genome annotation databases

EnsemblBacteriaiABA72090; ABA72090; Pfl01_0346
KEGGipfo:Pfl01_0346

Similar proteinsi

Entry informationi

Entry nameiTHII_PSEPF
AccessioniPrimary (citable) accession number: Q3KJG6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 8, 2005
Last modified: July 18, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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