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Protein

Pyridoxal 5'-phosphate synthase subunit PdxT

Gene

pdxT

Organism
Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / NBRC 14720 / NCIMB 2260 / Gabara) (Halobacterium pharaonis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.UniRule annotation

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.UniRule annotation
L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Pathwayi: pyridoxal 5'-phosphate biosynthesis

This protein is involved in the pathway pyridoxal 5'-phosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway pyridoxal 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85NucleophileUniRule annotation1
Binding sitei112L-glutamineUniRule annotation1
Active sitei176Charge relay systemUniRule annotation1
Active sitei178Charge relay systemUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciNPHA348780:G1GI2-241-MONOMER
UniPathwayiUPA00245

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxal 5'-phosphate synthase subunit PdxTUniRule annotation (EC:4.3.3.6UniRule annotation)
Alternative name(s):
Pdx2UniRule annotation
Pyridoxal 5'-phosphate synthase glutaminase subunitUniRule annotation (EC:3.5.1.2UniRule annotation)
Gene namesi
Name:pdxTUniRule annotation
Ordered Locus Names:NP_0464A
OrganismiNatronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / NBRC 14720 / NCIMB 2260 / Gabara) (Halobacterium pharaonis)
Taxonomic identifieri348780 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHaloarculaceaeNatronomonas
Proteomesi
  • UP000002698 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002558241 – 192Pyridoxal 5'-phosphate synthase subunit PdxTAdd BLAST192

Proteomic databases

PRIDEiQ3IU60

Interactioni

Subunit structurei

In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.UniRule annotation

Protein-protein interaction databases

STRINGi348780.NP0464A

Structurei

3D structure databases

ProteinModelPortaliQ3IU60
SMRiQ3IU60
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni53 – 55L-glutamine bindingUniRule annotation3
Regioni140 – 141L-glutamine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the glutaminase PdxT/SNO family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiarCOG00034 Archaea
COG0311 LUCA
HOGENOMiHOG000039949
KOiK08681
OMAiVFIRAPI
OrthoDBiPOG093Z0AY8

Family and domain databases

CDDicd01749 GATase1_PB, 1 hit
Gene3Di3.40.50.880, 1 hit
HAMAPiMF_01615 PdxT, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR002161 PdxT/SNO
PANTHERiPTHR31559 PTHR31559, 1 hit
PfamiView protein in Pfam
PF01174 SNO, 1 hit
PIRSFiPIRSF005639 Glut_amidoT_SNO, 1 hit
SUPFAMiSSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR03800 PLP_synth_Pdx2, 1 hit
PROSITEiView protein in PROSITE
PS51130 PDXT_SNO_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q3IU60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKAGVIAVQ GDVAEHADAI RRAADRHGED CTVESIRSAG VVPECDLLLL
60 70 80 90 100
PGGESTTISR LLAEEGIDEE IEAFAAAGKP LLATCAGLIV ASRDAKDDRV
110 120 130 140 150
STLDILDVSV DRNAFGRQKD SFEAAIDVEG LDEPFPAVFI RAPLIDEVDA
160 170 180 190
AVETLAAVDD RPVAVRQDNV VGTSFHPELT DDSRIHGLAF FS
Length:192
Mass (Da):20,379
Last modified:November 8, 2005 - v1
Checksum:i67874119D4442DA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR936257 Genomic DNA Translation: CAI48323.1
RefSeqiWP_011321959.1, NC_007426.1

Genome annotation databases

EnsemblBacteriaiCAI48323; CAI48323; NP_0464A
GeneIDi3700865
KEGGinph:NP_0464A

Similar proteinsi

Entry informationi

Entry nameiPDXT_NATPD
AccessioniPrimary (citable) accession number: Q3IU60
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: November 8, 2005
Last modified: June 20, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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