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Protein

DNA polymerase IV

Gene

dinB

Organism
Pseudoalteromonas haloplanktis (strain TAC 125)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi8MagnesiumUniRule annotation1
Sitei13Substrate discriminationUniRule annotation1
Metal bindingi102MagnesiumUniRule annotation1
Active sitei103UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciPHAL326442:G1GI4-2361-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase IVUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IVUniRule annotation
Gene namesi
Name:dinBUniRule annotation
Ordered Locus Names:PSHAa2389
OrganismiPseudoalteromonas haloplanktis (strain TAC 125)
Taxonomic identifieri326442 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas
Proteomesi
  • UP000006843 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000849141 – 351DNA polymerase IVAdd BLAST351

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi326442.PSHAa2389

Structurei

3D structure databases

ProteinModelPortaliQ3II35
SMRiQ3II35
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 184UmuCUniRule annotationAdd BLAST181

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQ3 Bacteria
COG0389 LUCA
HOGENOMiHOG000082707
KOiK02346
OMAiGMQIREI
OrthoDBiPOG091H014I

Family and domain databases

CDDicd03586 PolY_Pol_IV_kappa, 1 hit
Gene3Di3.30.1490.100, 1 hit
HAMAPiMF_01113 DNApol_IV, 1 hit
InterProiView protein in InterPro
IPR036775 DNA_pol_Y-fam_lit_finger_sf
IPR017961 DNA_pol_Y-fam_little_finger
IPR022880 DNApol_IV
IPR024728 PolY_HhH_motif
IPR001126 UmuC
PfamiView protein in Pfam
PF00817 IMS, 1 hit
PF11799 IMS_C, 1 hit
PF11798 IMS_HHH, 1 hit
SUPFAMiSSF100879 SSF100879, 1 hit
PROSITEiView protein in PROSITE
PS50173 UMUC, 1 hit

Sequencei

Sequence statusi: Complete.

Q3II35-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKFIHIDMD CFYAAVEMRD NPKLANVPLA IGGNSRRGVL STANYIAREY
60 70 80 90 100
GVRSAMSNYH AKQLCPDLVI VPGRMAVYKD VSTQIRAVFS RYTDLIEPLS
110 120 130 140 150
LDEAYLDVTH SEQCKGSATL IAQQIRADIY NATGLTASAG IAPIKFIAKI
160 170 180 190 200
ASDENKPNGQ FVVLPDQVDA FLAQLPLGKI PGVGKVTLEK LNLKGLYTGQ
210 220 230 240 250
DVRKKGVNWM QQHVGNFGVS LYQKCAGEHI GRVSTERVRK SLSVEHTYEY
260 270 280 290 300
NKNSLQECLE QLPSLLEELT TRLNKQQLQN QVNKLSVKVK FANFVVTSAD
310 320 330 340 350
QAHQQLNSDI FSELLAKAYQ RGLQQPVRLL GIGVGIKNQP QHNLQLSILD

M
Length:351
Mass (Da):39,000
Last modified:November 8, 2005 - v1
Checksum:i4397EEB3235EC60E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR954246 Genomic DNA Translation: CAI87438.1
RefSeqiWP_011329039.1, NC_007481.1

Genome annotation databases

EnsemblBacteriaiCAI87438; CAI87438; PSHAa2389
KEGGipha:PSHAa2389
PATRICifig|326442.8.peg.2302

Similar proteinsi

Entry informationi

Entry nameiDPO4_PSEHT
AccessioniPrimary (citable) accession number: Q3II35
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 8, 2005
Last modified: March 28, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health