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Protein

Probable protein S-acyltransferase 23

Gene

PAT23

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Palmitoyl acyltransferase.By similarity1 Publication

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei393S-palmitoyl cysteine intermediateBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.3.1.225 399

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein S-acyltransferase 23 (EC:2.3.1.225)
Alternative name(s):
Probable palmitoyltransferase At2g14255
Zinc finger DHHC domain-containing protein At2g14255
Gene namesi
Name:PAT23
Ordered Locus Names:At2g14255
ORF Names:T1O16.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G14255
TAIRilocus:1006230134 AT2G14255

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei270 – 290HelicalSequence analysisAdd BLAST21
Transmembranei298 – 318HelicalSequence analysisAdd BLAST21
Transmembranei407 – 427HelicalSequence analysisAdd BLAST21
Transmembranei454 – 474HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003635901 – 536Probable protein S-acyltransferase 23Add BLAST536

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiQ3EC11

Expressioni

Tissue specificityi

Expressed in roots, shoots, flowers and pollen.1 Publication

Gene expression databases

ExpressionAtlasiQ3EC11 baseline and differential
GenevisibleiQ3EC11 AT

Interactioni

Protein-protein interaction databases

BioGridi30135, 1 interactor
IntActiQ3EC11, 1 interactor
STRINGi3702.AT2G14255.1

Structurei

3D structure databases

ProteinModelPortaliQ3EC11
SMRiQ3EC11
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati57 – 86ANK 1Add BLAST30
Repeati90 – 119ANK 2Add BLAST30
Repeati123 – 153ANK 3Add BLAST31
Repeati157 – 186ANK 4Add BLAST30
Repeati190 – 219ANK 5Add BLAST30
Repeati225 – 254ANK 6Add BLAST30
Domaini363 – 413DHHCPROSITE-ProRule annotationAdd BLAST51

Domaini

The DHHC domain is required for palmitoyltransferase activity.By similarity

Sequence similaritiesi

Belongs to the DHHC palmitoyltransferase family.Curated

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0509 Eukaryota
COG0666 LUCA
COG5273 LUCA
HOGENOMiHOG000243854
InParanoidiQ3EC11
KOiK20032
OMAiHLTDING
OrthoDBiEOG093605DC
PhylomeDBiQ3EC11

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 2 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR001594 Palmitoyltrfase_DHHC
PfamiView protein in Pfam
PF12796 Ank_2, 2 hits
PF01529 DHHC, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 6 hits
SUPFAMiSSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 4 hits
PS50216 DHHC, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced.
Isoform 1 (identifier: Q3EC11-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSSEIEVVP LDSNSHQSPT ESPTITDVFS ASAYGDLHQL KHFVEHNGSS
60 70 80 90 100
VSLPDDNGFY ALQWAALNNS LHVAQYIIQH GGDVNSADNI QQTPLHWAAV
110 120 130 140 150
KGSIDVADLL LQHGARIEAV DVNGFRAVHV ASQYGQTAFV NHIIVDYAAD
160 170 180 190 200
YNALDIEGRS PLHWAAYNGF TETVRLLLFR DACQNRQDNT GCTPLHWAVI
210 220 230 240 250
KENVEACTLL VHAGTKEELI LKDNTGSTPL KLASDKGHRQ LALFLSKAMR
260 270 280 290 300
TRKNSFVDKI FCGKLGETSY APMLFSLIVI LMVLFITSIV SASNLPKITA
310 320 330 340 350
MVGLWACFGL SCGVYALITF YRVSRKDPGY VKRTGEANSQ HTANDPLIDI
360 370 380 390 400
NFKNPSWKGN WSQLCPTCKI IRPVRSKHCP TCKRCVEQFD HHCPWISNCV
410 420 430 440 450
GKKNKRYFLV FVIMGALTSF VGGTTAVQRL WRGIPQVHHG ESWIKHIVIE
460 470 480 490 500
HPDAAVFLFF DLLIFIATMT LTISQSYMIA RNITTNELWN AKRFSYLRGP
510 520 530
DGRFYNPYNH GLRRNCTDFL VHGYTRDDEV VPSSIL
Length:536
Mass (Da):59,988
Last modified:July 1, 2008 - v2
Checksum:i1BBB18C651003487
GO

Sequence cautioni

The sequence AAD20110 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti246 – 249SKAM → VRNV in ABN04785 (Ref. 3) Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006304 Genomic DNA Translation: AAD20110.1 Sequence problems.
CP002685 Genomic DNA Translation: AEC06292.1
BT030047 mRNA Translation: ABN04785.1
PIRiD84515
RefSeqiNP_973453.2, NM_201724.3 [Q3EC11-1]
UniGeneiAt.40568

Genome annotation databases

EnsemblPlantsiAT2G14255.1; AT2G14255.1; AT2G14255 [Q3EC11-1]
GeneIDi2745525
GrameneiAT2G14255.1; AT2G14255.1; AT2G14255 [Q3EC11-1]
KEGGiath:AT2G14255

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiZDHC2_ARATH
AccessioniPrimary (citable) accession number: Q3EC11
Secondary accession number(s): A2RVQ6, Q9ZQ58
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: July 1, 2008
Last modified: July 18, 2018
This is version 96 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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