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Protein

General transcription and DNA repair factor IIH subunit TFB5

Gene

TFB5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription. TFB5 is required for stable recruitment of TFIIH to a promoter, but not for stability of TFIIH subunits.2 Publications

GO - Biological processi

  • nucleotide-excision repair Source: SGD
  • phosphorylation of RNA polymerase II C-terminal domain Source: SGD
  • transcription by RNA polymerase II Source: SGD

Keywordsi

Biological processDNA damage, DNA repair, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30109-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription and DNA repair factor IIH subunit TFB5
Short name:
TFIIH subunit TFB5
Alternative name(s):
RNA polymerase II transcription factor B subunit 5
Gene namesi
Name:TFB5
Ordered Locus Names:YDR079C-A
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

SGDiS000007603 TFB5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192871 – 72General transcription and DNA repair factor IIH subunit TFB5Add BLAST72

Proteomic databases

MaxQBiQ3E7C1
PaxDbiQ3E7C1
PRIDEiQ3E7C1

PTM databases

iPTMnetiQ3E7C1

Interactioni

Subunit structurei

Component of the 7-subunit TFIIH core complex composed of XPB/SSL2, XPD/RAD3, SSL1, TFB1, TFB2, TFB4 and TFB5, which is active in NER. The core complex associates with the 3-subunit CTD-kinase module TFIIK composed of CCL1, KIN28 and TFB3 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription (PubMed:15220919). Interacts with TFB2 (PubMed:19172752).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TFB2Q029396EBI-2095127,EBI-2345544

Protein-protein interaction databases

BioGridi32135, 213 interactors
ComplexPortaliCPX-1659 General transcription factor complex TFIIH
DIPiDIP-48375N
IntActiQ3E7C1, 10 interactors
MINTiQ3E7C1
STRINGi4932.YDR079C-A

Structurei

Secondary structure

172
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ3E7C1
SMRiQ3E7C1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3E7C1

Family & Domainsi

Sequence similaritiesi

Belongs to the TFB5 family.Curated

Phylogenomic databases

HOGENOMiHOG000199698
InParanoidiQ3E7C1
KOiK10845
OMAiKVMQPEE
OrthoDBiEOG092C5WAI

Family and domain databases

Gene3Di3.30.70.1220, 1 hit
InterProiView protein in InterPro
IPR035935 TFB5-like_sf
IPR009400 TFIIH_TTDA/Tfb5
PANTHERiPTHR28580 PTHR28580, 1 hit
PfamiView protein in Pfam
PF06331 Tfb5, 1 hit
SMARTiView protein in SMART
SM01395 Tbf5, 1 hit
SUPFAMiSSF142897 SSF142897, 1 hit

Sequencei

Sequence statusi: Complete.

Q3E7C1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MARARKGALV QCDPSIKALI LQIDAKMSDI VLEELDDTHL LVNPSKVEFV
60 70
KHELNRLLSK NIYNPMDEEE NQ
Length:72
Mass (Da):8,232
Last modified:November 8, 2005 - v1
Checksum:i9198E5CCFF304239
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74376 Genomic DNA No translation available.
BK006938 Genomic DNA Translation: DAA11926.1
RefSeqiNP_076886.3, NM_001184469.3

Genome annotation databases

EnsemblFungiiYDR079C-A; YDR079C-A; YDR079C-A
GeneIDi851652
KEGGisce:YDR079C-A

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74376 Genomic DNA No translation available.
BK006938 Genomic DNA Translation: DAA11926.1
RefSeqiNP_076886.3, NM_001184469.3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DGPX-ray1.80B2-72[»]
3DOMX-ray2.60B/D2-72[»]
5FMFelectron microscopy6.00X2-64[»]
5OQJelectron microscopy4.7051-72[»]
5OQMelectron microscopy5.8051-72[»]
5SVAelectron microscopy15.30b1-72[»]
ProteinModelPortaliQ3E7C1
SMRiQ3E7C1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32135, 213 interactors
ComplexPortaliCPX-1659 General transcription factor complex TFIIH
DIPiDIP-48375N
IntActiQ3E7C1, 10 interactors
MINTiQ3E7C1
STRINGi4932.YDR079C-A

PTM databases

iPTMnetiQ3E7C1

Proteomic databases

MaxQBiQ3E7C1
PaxDbiQ3E7C1
PRIDEiQ3E7C1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR079C-A; YDR079C-A; YDR079C-A
GeneIDi851652
KEGGisce:YDR079C-A

Organism-specific databases

SGDiS000007603 TFB5

Phylogenomic databases

HOGENOMiHOG000199698
InParanoidiQ3E7C1
KOiK10845
OMAiKVMQPEE
OrthoDBiEOG092C5WAI

Enzyme and pathway databases

BioCyciYEAST:G3O-30109-MONOMER

Miscellaneous databases

EvolutionaryTraceiQ3E7C1
PROiPR:Q3E7C1

Family and domain databases

Gene3Di3.30.70.1220, 1 hit
InterProiView protein in InterPro
IPR035935 TFB5-like_sf
IPR009400 TFIIH_TTDA/Tfb5
PANTHERiPTHR28580 PTHR28580, 1 hit
PfamiView protein in Pfam
PF06331 Tfb5, 1 hit
SMARTiView protein in SMART
SM01395 Tbf5, 1 hit
SUPFAMiSSF142897 SSF142897, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTFB5_YEAST
AccessioniPrimary (citable) accession number: Q3E7C1
Secondary accession number(s): D6VS66
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: November 8, 2005
Last modified: November 7, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names
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Main funding by: National Institutes of Health

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