UniProtKB - Q3B8J8 (SDHB_XENLA)
Protein
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
Gene
sdhb
Organism
Xenopus laevis (African clawed frog)
Status
Functioni
Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).By similarity
Catalytic activityi
- EC:1.3.5.1
Cofactori
Protein has several cofactor binding sites:- [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster.By similarity
- [3Fe-4S] clusterBy similarityNote: Binds 1 [3Fe-4S] cluster.By similarity
- [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity
: tricarboxylic acid cycle Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes fumarate from succinate (eukaryal route).Proteins known to be involved in this subpathway in this organism are:
- Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (sdhb), Succinate dehydrogenase [ubiquinone] flavoprotein subunit B, mitochondrial (sdha-b), Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (XELAEV_18031324mg), Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (XELAEV_18033323mg), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (XELAEV_18037955mg), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (sdhb.L), Succinate dehydrogenase [ubiquinone] flavoprotein subunit A, mitochondrial (sdha-a)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes fumarate from succinate (eukaryal route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 95 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 100 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 103 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 115 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 188 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 191 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 194 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 198 | Iron-sulfur 3 (3Fe-4S)By similarity | 1 | |
Binding sitei | 203 | Ubiquinone; shared with dhsdBy similarity | 1 | |
Metal bindingi | 245 | Iron-sulfur 3 (3Fe-4S)By similarity | 1 | |
Metal bindingi | 251 | Iron-sulfur 3 (3Fe-4S)By similarity | 1 | |
Metal bindingi | 255 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB
- 3 iron, 4 sulfur cluster binding Source: UniProtKB
- 4 iron, 4 sulfur cluster binding Source: UniProtKB
- electron transfer activity Source: InterPro
- metal ion binding Source: UniProtKB-KW
- succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
- ubiquinone binding Source: UniProtKB
GO - Biological processi
- tricarboxylic acid cycle Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Transport, Tricarboxylic acid cycle |
Ligand | 2Fe-2S, 3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00223;UER01006 |
Names & Taxonomyi
Protein namesi | Recommended name: Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (EC:1.3.5.1)Alternative name(s): Iron-sulfur subunit of complex II Short name: Ip |
Gene namesi | Name:sdhb |
Organismi | Xenopus laevis (African clawed frog) |
Taxonomic identifieri | 8355 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus |
Organism-specific databases
Xenbasei | XB-GENE-970197, sdhb.S |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Matrix side By similarity
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB
- mitochondrial respiratory chain complex II, succinate dehydrogenase complex (ubiquinone) Source: UniProtKB
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 26 | MitochondrionBy similarityAdd BLAST | 26 | |
ChainiPRO_0000343802 | 27 – 282 | Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrialAdd BLAST | 256 |
Interactioni
Subunit structurei
Component of complex II composed of four subunits: the flavoprotein (FP) sdha, iron-sulfur protein (IP) sdhb, and a cytochrome b composed of sdhc and sdhd.
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 42 – 135 | 2Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST | 94 | |
Domaini | 178 – 208 | 4Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd BLAST | 31 |
Sequence similaritiesi
Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
OrthoDBi | 1264157at2759 |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
Gene3Di | 1.10.1060.10, 1 hit 3.10.20.30, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006058, 2Fe2S_fd_BS IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR012675, Beta-grasp_dom_sf IPR009051, Helical_ferredxn IPR004489, Succ_DH/fum_Rdtase_Fe-S IPR025192, Succ_DH/fum_Rdtase_N |
Pfami | View protein in Pfam PF13085, Fer2_3, 1 hit |
SUPFAMi | SSF46548, SSF46548, 1 hit SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR00384, dhsB, 1 hit |
PROSITEi | View protein in PROSITE PS00197, 2FE2S_FER_1, 1 hit PS51085, 2FE2S_FER_2, 1 hit PS00198, 4FE4S_FER_1, 1 hit PS51379, 4FE4S_FER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q3B8J8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAVVFSLRR SGPVLRLSGA LQVSRGAQTA AAPASQAAAR IKKFAIYRWD
60 70 80 90 100
PDKPGDKPRM QTYEVDLNTC GPMVLDALIK IKNEVDPTLT FRRSCREGIC
110 120 130 140 150
GSCAMNINGG NTLACTVRID TNLSKVSKIY PLPHMYVVKD LVPDLSNFYA
160 170 180 190 200
QYKSIEPYLK KKDKSQQGKE QYLQSIEDRD KLDGLYECIL CACCSTSCPS
210 220 230 240 250
YWWNADKYLG PAVLMQAYRW MIDSRDDFTE ERLSKLQDPF SLYRCHTIMN
260 270 280
CTRTCPKGLN PGKAIAEIKK MMAMYKERAV SA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 164 | K → E in AAH43859 (Ref. 1) Curated | 1 | |
Sequence conflicti | 279 | A → V in AAH43859 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC043859 mRNA Translation: AAH43859.1 BC106300 mRNA Translation: AAI06301.1 |
RefSeqi | NP_001080247.1, NM_001086778.1 |
Genome annotation databases
GeneIDi | 379939 |
KEGGi | xla:379939 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC043859 mRNA Translation: AAH43859.1 BC106300 mRNA Translation: AAI06301.1 |
RefSeqi | NP_001080247.1, NM_001086778.1 |
3D structure databases
SMRi | Q3B8J8 |
ModBasei | Search... |
Genome annotation databases
GeneIDi | 379939 |
KEGGi | xla:379939 |
Organism-specific databases
CTDi | 379939 |
Xenbasei | XB-GENE-970197, sdhb.S |
Phylogenomic databases
OrthoDBi | 1264157at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00223;UER01006 |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
Gene3Di | 1.10.1060.10, 1 hit 3.10.20.30, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006058, 2Fe2S_fd_BS IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR012675, Beta-grasp_dom_sf IPR009051, Helical_ferredxn IPR004489, Succ_DH/fum_Rdtase_Fe-S IPR025192, Succ_DH/fum_Rdtase_N |
Pfami | View protein in Pfam PF13085, Fer2_3, 1 hit |
SUPFAMi | SSF46548, SSF46548, 1 hit SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR00384, dhsB, 1 hit |
PROSITEi | View protein in PROSITE PS00197, 2FE2S_FER_1, 1 hit PS51085, 2FE2S_FER_2, 1 hit PS00198, 4FE4S_FER_1, 1 hit PS51379, 4FE4S_FER_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SDHB_XENLA | |
Accessioni | Q3B8J8Primary (citable) accession number: Q3B8J8 Secondary accession number(s): Q7ZYB6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 22, 2008 |
Last sequence update: | November 22, 2005 | |
Last modified: | December 2, 2020 | |
This is version 78 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families