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Protein

Polyubiquitin 8

Gene

UBQ8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity).By similarity

Miscellaneous

Ubiquitin is encoded by 16 different genes. Ubiquitin is generally synthesized as a polyubiquitin precursor with tandem head to tail repeats. Often, there is one to three additional amino acids after the last repeat, removed in the mature protein. Alternatively, ubiquitin extension protein is synthesized as a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27A) or to a ubiquitin-related protein (either RUB1 or RUB2). Following translation, extension protein is cleaved from ubiquitin.

GO - Biological processi

  • ubiquitin-dependent protein catabolic process Source: TAIR

Keywordsi

Biological processUbl conjugation pathway

Names & Taxonomyi

Protein namesi
Gene namesi
Name:UBQ8
Ordered Locus Names:At3g09790
ORF Names:F11F8_38, F8A24.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G09790
TAIRilocus:2074909 AT3G09790

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003969151 – 78Ubiquitin-related 1Add BLAST78
ChainiPRO_000039691679 – 154Ubiquitin-related 2Add BLAST76
ChainiPRO_0000396917155 – 237Ubiquitin-related 3Add BLAST83
ChainiPRO_0000396918238 – 318Ubiquitin-related 4Add BLAST81
ChainiPRO_0000396919319 – 392Ubiquitin-related 5Add BLAST74
ChainiPRO_0000396920393 – 468Ubiquitin-related 6Add BLAST76
ChainiPRO_0000396921469 – 551Ubiquitin-related 7Add BLAST83
ChainiPRO_0000396922552 – 627Ubiquitin-related 8Add BLAST76
PropeptideiPRO_0000396923628 – 631Curated4

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki78Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation
Cross-linki154Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation
Cross-linki551Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation

Keywords - PTMi

Isopeptide bond

Proteomic databases

PaxDbiQ39256
PRIDEiQ39256

Expressioni

Gene expression databases

ExpressionAtlasiQ39256 baseline and differential
GenevisibleiQ39256 AT

Interactioni

Protein-protein interaction databases

BioGridi5471, 2 interactors
STRINGi3702.AT3G09790.1

Structurei

3D structure databases

ProteinModelPortaliQ39256
SMRiQ39256
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 78Ubiquitin-like 1PROSITE-ProRule annotationAdd BLAST76
Domaini79 – 154Ubiquitin-like 2PROSITE-ProRule annotationAdd BLAST76
Domaini155 – 237Ubiquitin-like 3PROSITE-ProRule annotationAdd BLAST83
Domaini238 – 318Ubiquitin-like 4PROSITE-ProRule annotationAdd BLAST81
Domaini319 – 392Ubiquitin-like 5PROSITE-ProRule annotationAdd BLAST74
Domaini393 – 468Ubiquitin-like 6PROSITE-ProRule annotationAdd BLAST76
Domaini469 – 551Ubiquitin-like 7PROSITE-ProRule annotationAdd BLAST83
Domaini552 – 627Ubiquitin-like 8PROSITE-ProRule annotationAdd BLAST76

Sequence similaritiesi

Belongs to the ubiquitin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0001 Eukaryota
COG5272 LUCA
HOGENOMiHOG000233942
InParanoidiQ39256
KOiK08770
OMAiNLEVESW
OrthoDBiEOG093608U5
PhylomeDBiQ39256

Family and domain databases

InterProiView protein in InterPro
IPR019956 Ubiquitin
IPR029071 Ubiquitin-like_domsf
IPR000626 Ubiquitin_dom
PfamiView protein in Pfam
PF00240 ubiquitin, 8 hits
PRINTSiPR00348 UBIQUITIN
SMARTiView protein in SMART
SM00213 UBQ, 8 hits
SUPFAMiSSF54236 SSF54236, 8 hits
PROSITEiView protein in PROSITE
PS50053 UBIQUITIN_2, 8 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q39256-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIQIYAKTL TEKTITLDVE TSDSIHNVKA KIQNKEGIPL DQQRLIFAGK
60 70 80 90 100
QLEDGLTLAD YNIQKESTLH LVLRLRGGMQ IFVQTLTGKT ITLEVKSSDT
110 120 130 140 150
IDNVKAKIQD KEGILPRQQR LIFAGKQLED GRTLADYNIQ KESTLHLVLR
160 170 180 190 200
LCGGMQIFVS TFSGKNFTSD TLTLKVESSD TIENVKAKIQ DREGLRPDHQ
210 220 230 240 250
RLIFHGEELF TEDNRTLADY GIRNRSTLCL ALRLRGDMYI FVKNLPYNSF
260 270 280 290 300
TGENFILEVE SSDTIDNVKA KLQDKERIPM DLHRLIFAGK PLEGGRTLAH
310 320 330 340 350
YNIQKGSTLY LVTRFRCGMQ IFVKTLTRKR INLEVESWDT IENVKAMVQD
360 370 380 390 400
KEGIQPQPNL QRLIFLGKEL KDGCTLADYS IQKESTLHLV LGMQIFVKLF
410 420 430 440 450
GGKIITLEVL SSDTIKSVKA KIQDKVGSPP DQQILLFRGG QLQDGRTLGD
460 470 480 490 500
YNIRNESTLH LFFHIRHGMQ IFVKTFSFSG ETPTCKTITL EVESSDTIDN
510 520 530 540 550
VKVKIQHKVG IPLDRQRLIF GGRVLVGSRT LLDYNIQKGS TIHQLFLQRG
560 570 580 590 600
GMQIFIKTLT GKTIILEVES SDTIANVKEK IQVKEGIKPD QQMLIFFGQQ
610 620 630
LEDGVTLGDY DIHKKSTLYL VLRLRQRRYD F
Length:631
Mass (Da):71,769
Last modified:November 1, 1996 - v1
Checksum:iF9552A7065689D6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05917 Genomic DNA Translation: AAA68879.1
AC015985 Genomic DNA Translation: AAF23256.1
AC016661 Genomic DNA Translation: AAF23307.1
CP002686 Genomic DNA Translation: AEE74813.1
CP002686 Genomic DNA Translation: ANM63421.1
PIRiS55243
RefSeqiNP_001319513.1, NM_001337841.1
NP_566357.1, NM_111814.2
UniGeneiAt.65074

Genome annotation databases

EnsemblPlantsiAT3G09790.1; AT3G09790.1; AT3G09790
AT3G09790.2; AT3G09790.2; AT3G09790
GeneIDi820137
GrameneiAT3G09790.1; AT3G09790.1; AT3G09790
AT3G09790.2; AT3G09790.2; AT3G09790
KEGGiath:AT3G09790

Similar proteinsi

Entry informationi

Entry nameiUBQ8_ARATH
AccessioniPrimary (citable) accession number: Q39256
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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