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Protein

Serine--tRNA ligase, cytoplasmic

Gene

At5g27470

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).By similarity

Catalytic activityi

ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser).
ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec).

Pathwayi: selenocysteinyl-tRNA(Sec) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-seryl-tRNA(Sec) from L-serine and tRNA(Sec).
Proteins known to be involved in this subpathway in this organism are:
  1. Serine--tRNA ligase, chloroplastic/mitochondrial (OVA7), Serine--tRNA ligase, cytoplasmic (At5g27470)
This subpathway is part of the pathway selenocysteinyl-tRNA(Sec) biosynthesis, which is itself part of Aminoacyl-tRNA biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-seryl-tRNA(Sec) from L-serine and tRNA(Sec), the pathway selenocysteinyl-tRNA(Sec) biosynthesis and in Aminoacyl-tRNA biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei285ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei292SerineBy similarity1
Binding sitei396SerineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi269 – 271ATPBy similarity3
Nucleotide bindingi358 – 361ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00906; UER00895

Names & Taxonomyi

Protein namesi
Recommended name:
Serine--tRNA ligase, cytoplasmicCurated (EC:6.1.1.11Curated)
Alternative name(s):
Seryl-tRNA synthetaseCurated
Short name:
SerRSCurated
Seryl-tRNA(Ser/Sec) synthetaseCurated
Gene namesi
Ordered Locus Names:At5g27470
ORF Names:F21A20_180
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G27470
TAIRilocus:2146400 AT5G27470

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001221951 – 451Serine--tRNA ligase, cytoplasmicAdd BLAST451

Proteomic databases

PaxDbiQ39230
PRIDEiQ39230

PTM databases

iPTMnetiQ39230

Expressioni

Gene expression databases

ExpressionAtlasiQ39230 baseline and differential
GenevisibleiQ39230 AT

Interactioni

Subunit structurei

Homodimer. The tRNA molecule binds across the dimer (By similarity).By similarity

Protein-protein interaction databases

STRINGi3702.AT5G27470.1

Structurei

3D structure databases

ProteinModelPortaliQ39230
SMRiQ39230
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni238 – 240Serine bindingBy similarity3

Domaini

Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2509 Eukaryota
COG0172 LUCA
HOGENOMiHOG000035937
InParanoidiQ39230
KOiK01875
OMAiSPCFRRE
OrthoDBiEOG093608ZS
PhylomeDBiQ39230

Family and domain databases

CDDicd00770 SerRS_core, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR006195 aa-tRNA-synth_II
IPR002317 Ser-tRNA-ligase_type_1
IPR015866 Ser-tRNA-synth_1_N
IPR033729 SerRS_core
IPR010978 tRNA-bd_arm
PANTHERiPTHR11778 PTHR11778, 1 hit
PfamiView protein in Pfam
PF02403 Seryl_tRNA_N, 1 hit
PF00587 tRNA-synt_2b, 1 hit
PIRSFiPIRSF001529 Ser-tRNA-synth_IIa, 1 hit
PRINTSiPR00981 TRNASYNTHSER
SUPFAMiSSF46589 SSF46589, 1 hit
TIGRFAMsiTIGR00414 serS, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

Q39230-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDINLFREE KGNNPEIIRE SQRRRFASVE IVDEIIKLDK EWRQRQFEVD
60 70 80 90 100
SFRKEFNKLN KQVAQLKIKK EDASEIIQQT EKNKQDSTAK EAEVREAYAA
110 120 130 140 150
LKAKLEQVGN LVHDSVPVDK DEANNLVIKL WGEKRFSTPG LKLKNHVDLV
160 170 180 190 200
ELLGIADTKR GAEIAGARGF FLKGDGLMLN QALINFGLTF LKKRGFTGLQ
210 220 230 240 250
PPFFMRKDVM AKCAQLAQFD EELYKVTGEG DDKYLIATAE QPLCAYHIDE
260 270 280 290 300
WIHPTELPLR YAGYSSCFRK EAGSHGRDTL GIFRVHQFEK IEQFCITGPN
310 320 330 340 350
ENASWEMLDE MMKNSEDFYQ ALKLPYQIVS IVSGALNDAA AKKYDLEAWF
360 370 380 390 400
PSSETFRELV SCSNCTDYQA RRLEIRYGQK KSNEQTKQYV HMLNSTLTAT
410 420 430 440 450
ERTICCILEN YQREDGVDIP EVLQPFMGGE TFLPFKAKPV VADTKGKKSK

A
Length:451
Mass (Da):51,629
Last modified:November 1, 1996 - v1
Checksum:iB13D71EF87236AAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70313 mRNA Translation: CAA94388.1
AC007123 Genomic DNA No translation available.
CP002688 Genomic DNA Translation: AED93692.1
AF360352 mRNA Translation: AAK28648.1
AY051054 mRNA Translation: AAK93731.1
PIRiS71293
RefSeqiNP_198099.1, NM_122629.3
UniGeneiAt.24387

Genome annotation databases

EnsemblPlantsiAT5G27470.1; AT5G27470.1; AT5G27470
GeneIDi832806
GrameneiAT5G27470.1; AT5G27470.1; AT5G27470
KEGGiath:AT5G27470

Similar proteinsi

Entry informationi

Entry nameiSYSC_ARATH
AccessioniPrimary (citable) accession number: Q39230
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

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