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Entry version 142 (18 Sep 2019)
Sequence version 2 (25 Jul 2003)
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Protein

Delta(7)-sterol-C5(6)-desaturase 1

Gene

STE1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in the biosynthesis of sitosterol and campesterol, a precursor of growth-promoting brassinosteroids.2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Fe cationBy similarity

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=32 µM for Delta7-cholesterol1 Publication
  1. Vmax=2.6 nmol/h/mg enzyme with Delta7-cholesterol as substrate1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandIron

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

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BioCyci
ARA:AT3G02580-MONOMER

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
1.14.21.6 399

SABIO-RK: Biochemical Reaction Kinetics Database

More...
SABIO-RKi
Q39208

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Delta(7)-sterol-C5(6)-desaturase 1 (EC:1.14.19.203 Publications)
Alternative name(s):
Delta(7)-sterol-C5-desaturase 1
Delta-7-C-5 sterol desaturase 1
Protein DWARF 7
Protein STEROL 1
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:STE11 Publication
Synonyms:DWF71 Publication
Ordered Locus Names:At3g02580
ORF Names:F16B3.21
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiArabidopsis thaliana (Mouse-ear cress)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri3702 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliopsidaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000006548 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 3

Organism-specific databases

Arabidopsis Information Portal

More...
Araporti
AT3G02580

The Arabidopsis Information Resource

More...
TAIRi
locus:2076884 AT3G02580

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei46 – 66HelicalSequence analysisAdd BLAST21
Transmembranei123 – 143HelicalSequence analysisAdd BLAST21
Transmembranei193 – 213HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi114T → I in ste1-1; 85% activity loss. 1 Publication1
Mutagenesisi114T → S: Increased maximal velocity and KM value of the enzyme. 1 Publication1
Mutagenesisi115K → L: No effect. 1 Publication1
Mutagenesisi147H → L or E: 100% activity loss. 1 Publication1
Mutagenesisi151H → L or E: 100% activity loss. 1 Publication1
Mutagenesisi161H → L or E: 100% activity loss. 1 Publication1
Mutagenesisi164H → L: 100% activity loss. 1 Publication1
Mutagenesisi165H → L: 100% activity loss. 1 Publication1
Mutagenesisi175P → A: No effect. 1 Publication1
Mutagenesisi175P → V: Increased catalytic efficiency and maximal velocity of the enzyme. 1 Publication1
Mutagenesisi201P → A: 80% activity loss. 1 Publication1
Mutagenesisi203H → L: 85% activity loss. 1 Publication1
Mutagenesisi222H → E: 93% activity loss. 1 Publication1
Mutagenesisi222H → L: 100% activity loss. 1 Publication1
Mutagenesisi230 – 281Missing in dwf7-1; loss of function resulting in a dwarf phenotype. Add BLAST52
Mutagenesisi234G → A: 100% activity loss. 1 Publication1
Mutagenesisi234G → D: 98% activity loss. 1 Publication1
Mutagenesisi238H → L or E: 100% activity loss. 1 Publication1
Mutagenesisi241H → L: 100% activity loss. 1
Mutagenesisi242H → L: 100% activity loss. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001170301 – 281Delta(7)-sterol-C5(6)-desaturase 1Add BLAST281

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q39208

PRoteomics IDEntifications database

More...
PRIDEi
Q39208

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
Q39208 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
Q39208 AT

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
3702.AT3G02580.1

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini133 – 262Fatty acid hydroxylaseSequence analysisAdd BLAST130

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi147 – 151Histidine box-15
Motifi161 – 165Histidine box-25
Motifi238 – 242Histidine box-35

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The histidine box domains may contain the active site and/or be involved in metal ion binding.

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the sterol desaturase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG0872 Eukaryota
COG3000 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000237470

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q39208

KEGG Orthology (KO)

More...
KOi
K00227

Identification of Orthologs from Complete Genome Data

More...
OMAi
HTLHHMY

Database of Orthologous Groups

More...
OrthoDBi
1249774at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q39208

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR006694 Fatty_acid_hydroxylase

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF04116 FA_hydroxylase, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q39208-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAADNAYLMQ FVDETSFYNR IVLSHLLPAN LWEPLPHFLQ TWLRNYLAGT
60 70 80 90 100
LLYFISGFLW CFYIYYLKIN VYLPKDAIPT IKAMRLQMFV AMKAMPWYTL
110 120 130 140 150
LPTVSESMIE RGWTKCFASI GEFGWILYFV YIAIYLVFVE FGIYWMHREL
160 170 180 190 200
HDIKPLYKYL HATHHIYNKQ NTLSPFAGLA FHPVDGILQA VPHVIALFIV
210 220 230 240 250
PIHFTTHIGL LFMEAIWTAN IHDCIHGNIW PVMGAGYHTI HHTTYKHNYG
260 270 280
HYTIWMDWMF GSLRDPLLEE DDNKDSFKKA E
Length:281
Mass (Da):33,143
Last modified:July 25, 2003 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iD32BB1BCDC4467D7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti54F → I in CAA62079 (PubMed:8919915).Curated1
Sequence conflicti67L → R in CAA62079 (PubMed:8919915).Curated1
Sequence conflicti121G → D in AAD38120 (PubMed:9927639).Curated1
Sequence conflicti218T → A in CAA62079 (PubMed:8919915).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X90454 mRNA Translation: CAA62079.1
AF105034 Genomic DNA Translation: AAD38120.1
AF069468 Genomic DNA Translation: AAD12944.1
AC021640 Genomic DNA Translation: AAF32465.1
CP002686 Genomic DNA Translation: AEE73832.1
AY088527 mRNA Translation: AAM66060.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S71251

NCBI Reference Sequences

More...
RefSeqi
NP_186907.1, NM_111126.4

Genome annotation databases

Ensembl plant genome annotation project

More...
EnsemblPlantsi
AT3G02580.1; AT3G02580.1; AT3G02580

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
820679

Gramene; a comparative resource for plants

More...
Gramenei
AT3G02580.1; AT3G02580.1; AT3G02580

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ath:AT3G02580

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X90454 mRNA Translation: CAA62079.1
AF105034 Genomic DNA Translation: AAD38120.1
AF069468 Genomic DNA Translation: AAD12944.1
AC021640 Genomic DNA Translation: AAF32465.1
CP002686 Genomic DNA Translation: AEE73832.1
AY088527 mRNA Translation: AAM66060.1
PIRiS71251
RefSeqiNP_186907.1, NM_111126.4

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

STRINGi3702.AT3G02580.1

Proteomic databases

PaxDbiQ39208
PRIDEiQ39208

Genome annotation databases

EnsemblPlantsiAT3G02580.1; AT3G02580.1; AT3G02580
GeneIDi820679
GrameneiAT3G02580.1; AT3G02580.1; AT3G02580
KEGGiath:AT3G02580

Organism-specific databases

AraportiAT3G02580
TAIRilocus:2076884 AT3G02580

Phylogenomic databases

eggNOGiKOG0872 Eukaryota
COG3000 LUCA
HOGENOMiHOG000237470
InParanoidiQ39208
KOiK00227
OMAiHTLHHMY
OrthoDBi1249774at2759
PhylomeDBiQ39208

Enzyme and pathway databases

BioCyciARA:AT3G02580-MONOMER
BRENDAi1.14.21.6 399
SABIO-RKiQ39208

Miscellaneous databases

Protein Ontology

More...
PROi
PR:Q39208

Gene expression databases

ExpressionAtlasiQ39208 baseline and differential
GenevisibleiQ39208 AT

Family and domain databases

InterProiView protein in InterPro
IPR006694 Fatty_acid_hydroxylase
PfamiView protein in Pfam
PF04116 FA_hydroxylase, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSC5D1_ARATH
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q39208
Secondary accession number(s): Q8L9C0, Q9SWU4, Q9SYS2
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: July 25, 2003
Last modified: September 18, 2019
This is version 142 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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