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Protein

Transcription factor MYC2

Gene

MYC2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator. Common transcription factor of light, abscisic acid (ABA), and jasmonic acid (JA) signaling pathways. With MYC3 and MYC4, controls additively subsets of JA-dependent responses. In cooperation with MYB2 is involved in the regulation of ABA-inducible genes under drought stress conditions. Can form complexes with all known glucosinolate-related MYBs to regulate glucosinolate biosynthesis. Binds to the MYC recognition site (5'-CACATG-3'), and to the G-box (5'-CACNTG-3') and Z-box (5'-ATACGTGT-3') of promoters. Binds directly to the promoters of the transcription factors PLETHORA1 (PLT1) and PLT2 and represses their expression. Negative regulator of blue light-mediated photomorphogenic growth and blue- and far-red-light regulated gene expression. Activates multiple TIFY/JAZ promoters. Positive regulator of lateral root formation. Regulates sesquiterpene biosynthesis. Subjected to proteasome-dependent proteolysis. The presence of the destruction element (DE) involved in turnover is required for the function to regulate gene transcription.11 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • DNA-binding transcription factor activity Source: TAIR
  • protein dimerization activity Source: InterPro
  • sequence-specific DNA binding Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processAbscisic acid signaling pathway, Jasmonic acid signaling pathway, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor MYC2
Short name:
AtMYC2
Alternative name(s):
Basic helix-loop-helix protein 6
Short name:
AtbHLH6
Short name:
bHLH 6
Protein JASMONATE INSENSITIVE 1
R-homologous Arabidopsis protein 1
Short name:
RAP-1
Transcription factor EN 38
Z-box binding factor 1 protein
bHLH transcription factor bHLH006
rd22BP1
Gene namesi
Name:MYC2
Synonyms:BHLH6, EN38, JAI1, JIN1, RAP1, RD22BP1, ZBF1
Ordered Locus Names:At1g32640
ORF Names:F6N18.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G32640
TAIRilocus:2035609 AT1G32640

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Minor effect on jasmonic acid response and no effect on glucosinolate biosynthesis, but decreased abscisic acid sensitivity. Myc2 and myc3 double mutant has an increased insensitivity to jasmonic acid. Myc2 and myc4 double mutant has an increased insensitivity to jasmonic acid. Myc2, myc3 and myc4 triple mutant has no jasmonate-related defense response, is devoid of glucosinolates and is extremely susceptible to generalist herbivores.4 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi328T → A: Jasmonate-insensitive phenotype and increased stability of the protein. 1 Publication1
Mutagenesisi330S → A: No effect. 1 Publication1
Mutagenesisi334S → A: No effect. 1 Publication1
Mutagenesisi336T → A: No effect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001274271 – 623Transcription factor MYC2Add BLAST623

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei328Phosphothreonine1 Publication1

Post-translational modificationi

The phosphorylation at Thr-328 is increased by methyl jasmonate treatment, facilitates the proteolysis of the protein and is coupled to transcription activity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39204

PTM databases

iPTMnetiQ39204

Expressioni

Tissue specificityi

Widely expressed in the whole plant with the highest expression in stem. Constitutively expressed in dark- and light-grown seedlings.2 Publications

Inductioni

Detected early after abscisic acid (ABA) treatment or after dehydration and high-salt stresses. Induced by UV treatment. Up-regulated by methyl jasmonate and herbivory.5 Publications

Gene expression databases

ExpressionAtlasiQ39204 differential
GenevisibleiQ39204 AT

Interactioni

Subunit structurei

Homotetramer. Tetramerization enhances DNA binding affinity (PubMed:28514654). Interacts (via N-terminus) with RGA, (via TAD domain) with the mediator subunit MED25, with TIC, MYC3, AFPH2/NINJA and the JAZ repressors TIFY10A/JAZ1, TIFY10B/JAZ2, TIFY6B/JAZ3, TIFY6A/JAZ4, TIFY11A/JAZ5, TIFY11B/JAZ6, TIFY5A/JAZ8, TIFY7/JAZ9, TIFY9/JAZ10, TIFY3A/JAZ11 and TIFY3B/JAZ12. Interacts with JAZ13 (via jas motif) (PubMed:25846245). Interacts with MYB28, MYB29, MYB34, MYB51, MYB76, MYB122 and mybe MYB2. Interacts with PYL6 in the nucleus. Interaction with PYL6 is increased in the presence of abscisic acid (PubMed:27357749).By similarity10 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi25392, 48 interactors
DIPiDIP-40533N
IntActiQ39204, 17 interactors
STRINGi3702.AT1G32640.1

Structurei

Secondary structure

1623
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ39204
SMRiQ39204
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini448 – 497bHLHPROSITE-ProRule annotationAdd BLAST50

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni93 – 160JAZ-interaction domainAdd BLAST68

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi434 – 442Nuclear localization signalSequence analysis9

Domaini

The transcriptional activation domain (TAD) (149-188) overlaps with the destruction element (DE) (154-165).1 Publication
The JAZ-interaction domain (JID) (93-160) is sufficient for interaction with MYB proteins and most of the TIFY/JAZ proteins (PubMed:21335373 and PubMed:23943862).
The C-terminal half contains an active nuclear localization signal (NLS).1 Publication

Phylogenomic databases

eggNOGiENOG410IGFU Eukaryota
ENOG410YCJJ LUCA
HOGENOMiHOG000238207
InParanoidiQ39204
KOiK13422
OMAiPSMWIND
OrthoDBiEOG093607T9
PhylomeDBiQ39204

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR025610 MYC/MYB_N
PfamiView protein in Pfam
PF14215 bHLH-MYC_N, 1 hit
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequencei

Sequence statusi: Complete.

Q39204-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTDYRLQPTM NLWTTDDNAS MMEAFMSSSD ISTLWPPAST TTTTATTETT
60 70 80 90 100
PTPAMEIPAQ AGFNQETLQQ RLQALIEGTH EGWTYAIFWQ PSYDFSGASV
110 120 130 140 150
LGWGDGYYKG EEDKANPRRR SSSPPFSTPA DQEYRKKVLR ELNSLISGGV
160 170 180 190 200
APSDDAVDEE VTDTEWFFLV SMTQSFACGA GLAGKAFATG NAVWVSGSDQ
210 220 230 240 250
LSGSGCERAK QGGVFGMHTI ACIPSANGVV EVGSTEPIRQ SSDLINKVRI
260 270 280 290 300
LFNFDGGAGD LSGLNWNLDP DQGENDPSMW INDPIGTPGS NEPGNGAPSS
310 320 330 340 350
SSQLFSKSIQ FENGSSSTIT ENPNLDPTPS PVHSQTQNPK FNNTFSRELN
360 370 380 390 400
FSTSSSTLVK PRSGEILNFG DEGKRSSGNP DPSSYSGQTQ FENKRKRSMV
410 420 430 440 450
LNEDKVLSFG DKTAGESDHS DLEASVVKEV AVEKRPKKRG RKPANGREEP
460 470 480 490 500
LNHVEAERQR REKLNQRFYA LRAVVPNVSK MDKASLLGDA IAYINELKSK
510 520 530 540 550
VVKTESEKLQ IKNQLEEVKL ELAGRKASAS GGDMSSSCSS IKPVGMEIEV
560 570 580 590 600
KIIGWDAMIR VESSKRNHPA ARLMSALMDL ELEVNHASMS VVNDLMIQQA
610 620
TVKMGFRIYT QEQLRASLIS KIG
Length:623
Mass (Da):67,950
Last modified:August 22, 2003 - v2
Checksum:i4A8D4DE34C5928F6
GO

Sequence cautioni

The sequence BAA25078 differs from that shown. Reason: Frameshift at positions 353 and 360.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti258A → D in BAA25078 (PubMed:9368419).Curated1
Sequence conflicti526K → R in AAL55713 (PubMed:11130712).Curated1
Sequence conflicti529A → P in BAA25078 (PubMed:9368419).Curated1
Sequence conflicti612E → D in BAA25078 (PubMed:9368419).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99548 mRNA Translation: CAA67885.2
AB000875 mRNA Translation: BAA25078.1 Frameshift.
AJ843256 mRNA Translation: CAH58735.1
AC017118 Genomic DNA Translation: AAF25980.1
CP002684 Genomic DNA Translation: AEE31513.1
AY037203 mRNA Translation: AAK59788.1
BT003042 mRNA Translation: AAO23607.1
AF251691 mRNA Translation: AAL55713.1
PIRiT52293
RefSeqiNP_174541.1, NM_102998.4
UniGeneiAt.22648

Genome annotation databases

EnsemblPlantsiAT1G32640.1; AT1G32640.1; AT1G32640
GeneIDi840158
GrameneiAT1G32640.1; AT1G32640.1; AT1G32640
KEGGiath:AT1G32640

Similar proteinsi

Entry informationi

Entry nameiMYC2_ARATH
AccessioniPrimary (citable) accession number: Q39204
Secondary accession number(s): O48644
, Q5K1L7, Q8W2F4, Q9LPJ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: August 22, 2003
Last modified: September 12, 2018
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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