We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - Q39161 (NIR_ARATH)
Protein
Ferredoxin--nitrite reductase, chloroplastic
Gene
NIR1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Catalyzes the six-electron reduction of nitrite to ammonium.
1 PublicationCatalytic activityi
- EC:1.7.7.1
Cofactori
Protein has several cofactor binding sites:- sirohemeNote: Binds 1 siroheme per subunit.
- [4Fe-4S] clusterNote: Binds 1 [4Fe-4S] cluster per subunit.
: nitrate reduction (assimilation) Pathwayi
This protein is involved in the pathway nitrate reduction (assimilation), which is part of Nitrogen metabolism.View all proteins of this organism that are known to be involved in the pathway nitrate reduction (assimilation) and in Nitrogen metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 464 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 470 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 505 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 509 | Iron (siroheme axial ligand)By similarity | 1 | |
Metal bindingi | 509 | Iron-sulfur (4Fe-4S)By similarity | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- ferredoxin-nitrite reductase activity Source: UniProtKB-EC
- heme binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
- nitrite reductase (NO-forming) activity Source: TAIR
GO - Biological processi
- nitrate assimilation Source: UniProtKB-UniPathway
- response to nitrate Source: TAIR
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Nitrate assimilation, Transport |
Ligand | 4Fe-4S, Heme, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00653 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:NIR1 Synonyms:NIR Ordered Locus Names:At2g15620 ORF Names:F9O13.17 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G15620 |
TAIRi | locus:2053654, AT2G15620 |
Subcellular locationi
Chloroplast
- chloroplast 1 Publication
Chloroplast
- chloroplast Source: TAIR
- chloroplast stroma Source: TAIR
Extracellular region or secreted
- apoplast Source: TAIR
Mitochondrion
- mitochondrion Source: TAIR
Keywords - Cellular componenti
Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 25 | ChloroplastSequence analysisAdd BLAST | 25 | |
ChainiPRO_0000019703 | 26 – 586 | Ferredoxin--nitrite reductase, chloroplasticAdd BLAST | 561 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 103 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources |
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | Q39161 |
PRIDEi | Q39161 |
ProteomicsDBi | 236829 |
PTM databases
iPTMneti | Q39161 |
MetOSitei | Q39161 |
Expressioni
Inductioni
By nitrate and by light.1 Publication
Gene expression databases
ExpressionAtlasi | Q39161, baseline and differential |
Genevisiblei | Q39161, AT |
Interactioni
Subunit structurei
Monomer.
Protein-protein interaction databases
BioGRIDi | 1414, 6 interactors |
IntActi | Q39161, 2 interactors |
STRINGi | 3702.AT2G15620.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 20 | DisorderedSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.Curated
Keywords - Domaini
Repeat, Transit peptidePhylogenomic databases
eggNOGi | KOG0560, Eukaryota |
HOGENOMi | CLU_015667_2_2_1 |
InParanoidi | Q39161 |
OMAi | PGTDTCN |
OrthoDBi | 333458at2759 |
PhylomeDBi | Q39161 |
Family and domain databases
Gene3Di | 3.30.413.10, 2 hits |
InterProi | View protein in InterPro IPR005117, NiRdtase/SiRdtase_haem-b_fer IPR036136, Nit/Sulf_reduc_fer-like_dom_sf IPR006067, NO2/SO3_Rdtase_4Fe4S_dom IPR045854, NO2/SO3_Rdtase_4Fe4S_sf IPR006066, NO2/SO3_Rdtase_FeS/sirohaem_BS |
Pfami | View protein in Pfam PF01077, NIR_SIR, 2 hits PF03460, NIR_SIR_ferr, 2 hits |
PRINTSi | PR00397, SIROHAEM |
SUPFAMi | SSF55124, SSF55124, 2 hits SSF56014, SSF56014, 2 hits |
PROSITEi | View protein in PROSITE PS00365, NIR_SIR, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q39161-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTSFSLTFTS PLLPSSSTKP KRSVLVAAAQ TTAPAESTAS VDADRLEPRV
60 70 80 90 100
ELKDGFFILK EKFRKGINPQ EKVKIEREPM KLFMENGIEE LAKKSMEELD
110 120 130 140 150
SEKSSKDDID VRLKWLGLFH RRKHQYGKFM MRLKLPNGVT TSAQTRYLAS
160 170 180 190 200
VIRKYGEDGC ADVTTRQNWQ IRGVVLPDVP EILKGLASVG LTSLQSGMDN
210 220 230 240 250
VRNPVGNPIA GIDPEEIVDT RPYTNLLSQF ITANSQGNPD FTNLPRKWNV
260 270 280 290 300
CVVGTHDLYE HPHINDLAYM PANKDGRFGF NLLVGGFFSP KRCEEAIPLD
310 320 330 340 350
AWVPADDVLP LCKAVLEAYR DLGTRGNRQK TRMMWLIDEL GVEGFRTEVE
360 370 380 390 400
KRMPNGKLER GSSEDLVNKQ WERRDYFGVN PQKQEGLSFV GLHVPVGRLQ
410 420 430 440 450
ADDMDELARL ADTYGSGELR LTVEQNIIIP NVETSKTEAL LQEPFLKNRF
460 470 480 490 500
SPEPSILMKG LVACTGSQFC GQAIIETKLR ALKVTEEVER LVSVPRPIRM
510 520 530 540 550
HWTGCPNTCG QVQVADIGFM GCLTRGEEGK PVEGADVYVG GRIGSDSHIG
560 570 580
EIYKKGVRVT ELVPLVAEIL IKEFGAVPRE REENED
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 77 | R → G in AAN31831 (PubMed:14593172).Curated | 1 | |
Sequence conflicti | 214 | P → Q in AAN31830 (PubMed:14593172).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D14824 Genomic DNA Translation: BAA03561.1 AB006032 mRNA Translation: BAA21672.1 AC006248 Genomic DNA Translation: AAD17406.1 CP002685 Genomic DNA Translation: AEC06424.1 AF360320 mRNA Translation: AAK26030.1 AY045608 mRNA Translation: AAK73966.1 AY093995 mRNA Translation: AAM16256.1 AY142685 mRNA Translation: AAN13223.1 BT000685 mRNA Translation: AAN31830.1 BT000686 mRNA Translation: AAN31831.1 |
PIRi | C84531 |
RefSeqi | NP_179164.1, NM_127123.3 |
Genome annotation databases
EnsemblPlantsi | AT2G15620.1; AT2G15620.1; AT2G15620 |
GeneIDi | 816055 |
Gramenei | AT2G15620.1; AT2G15620.1; AT2G15620 |
KEGGi | ath:AT2G15620 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D14824 Genomic DNA Translation: BAA03561.1 AB006032 mRNA Translation: BAA21672.1 AC006248 Genomic DNA Translation: AAD17406.1 CP002685 Genomic DNA Translation: AEC06424.1 AF360320 mRNA Translation: AAK26030.1 AY045608 mRNA Translation: AAK73966.1 AY093995 mRNA Translation: AAM16256.1 AY142685 mRNA Translation: AAN13223.1 BT000685 mRNA Translation: AAN31830.1 BT000686 mRNA Translation: AAN31831.1 |
PIRi | C84531 |
RefSeqi | NP_179164.1, NM_127123.3 |
3D structure databases
SMRi | Q39161 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 1414, 6 interactors |
IntActi | Q39161, 2 interactors |
STRINGi | 3702.AT2G15620.1 |
PTM databases
iPTMneti | Q39161 |
MetOSitei | Q39161 |
Proteomic databases
PaxDbi | Q39161 |
PRIDEi | Q39161 |
ProteomicsDBi | 236829 |
Genome annotation databases
EnsemblPlantsi | AT2G15620.1; AT2G15620.1; AT2G15620 |
GeneIDi | 816055 |
Gramenei | AT2G15620.1; AT2G15620.1; AT2G15620 |
KEGGi | ath:AT2G15620 |
Organism-specific databases
Araporti | AT2G15620 |
TAIRi | locus:2053654, AT2G15620 |
Phylogenomic databases
eggNOGi | KOG0560, Eukaryota |
HOGENOMi | CLU_015667_2_2_1 |
InParanoidi | Q39161 |
OMAi | PGTDTCN |
OrthoDBi | 333458at2759 |
PhylomeDBi | Q39161 |
Enzyme and pathway databases
UniPathwayi | UPA00653 |
Miscellaneous databases
PROi | PR:Q39161 |
Gene expression databases
ExpressionAtlasi | Q39161, baseline and differential |
Genevisiblei | Q39161, AT |
Family and domain databases
Gene3Di | 3.30.413.10, 2 hits |
InterProi | View protein in InterPro IPR005117, NiRdtase/SiRdtase_haem-b_fer IPR036136, Nit/Sulf_reduc_fer-like_dom_sf IPR006067, NO2/SO3_Rdtase_4Fe4S_dom IPR045854, NO2/SO3_Rdtase_4Fe4S_sf IPR006066, NO2/SO3_Rdtase_FeS/sirohaem_BS |
Pfami | View protein in Pfam PF01077, NIR_SIR, 2 hits PF03460, NIR_SIR_ferr, 2 hits |
PRINTSi | PR00397, SIROHAEM |
SUPFAMi | SSF55124, SSF55124, 2 hits SSF56014, SSF56014, 2 hits |
PROSITEi | View protein in PROSITE PS00365, NIR_SIR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NIR_ARATH | |
Accessioni | Q39161Primary (citable) accession number: Q39161 Secondary accession number(s): Q8H163, Q8H164 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families