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Protein

Ferredoxin--nitrite reductase, chloroplastic

Gene

NIR1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the six-electron reduction of nitrite to ammonium.1 Publication

Catalytic activityi

NH3 + 2 H2O + 6 oxidized ferredoxin = nitrite + 6 reduced ferredoxin + 7 H+.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: nitrate reduction (assimilation)

This protein is involved in the pathway nitrate reduction (assimilation), which is part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the pathway nitrate reduction (assimilation) and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi464Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi470Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi505Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi509Iron (siroheme axial ligand)By similarity1
Metal bindingi509Iron-sulfur (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

  • nitrate assimilation Source: UniProtKB-UniPathway
  • response to nitrate Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Heme, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayi
UPA00653

Names & Taxonomyi

Protein namesi
Recommended name:
Ferredoxin--nitrite reductase, chloroplastic (EC:1.7.7.1)
Short name:
NiR
Gene namesi
Name:NIR1
Synonyms:NIR
Ordered Locus Names:At2g15620
ORF Names:F9O13.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G15620
TAIRilocus:2053654 AT2G15620

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 25ChloroplastSequence analysisAdd BLAST25
ChainiPRO_000001970326 – 586Ferredoxin--nitrite reductase, chloroplasticAdd BLAST561

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki103Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ39161
PRIDEiQ39161

PTM databases

iPTMnetiQ39161

Expressioni

Inductioni

By nitrate and by light.1 Publication

Gene expression databases

ExpressionAtlasiQ39161 baseline and differential
GenevisibleiQ39161 AT

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi1414, 3 interactors
IntActiQ39161, 2 interactors
STRINGi3702.AT2G15620.1

Structurei

3D structure databases

ProteinModelPortaliQ39161
SMRiQ39161
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG0560 Eukaryota
COG0155 LUCA
HOGENOMiHOG000249408
InParanoidiQ39161
KOiK00366
OMAiCNFALIE
OrthoDBiEOG093604GV
PhylomeDBiQ39161

Family and domain databases

InterProiView protein in InterPro
IPR005117 NiRdtase/SiRdtase_haem-b_fer
IPR036136 Nit/Sulf_reduc_fer_like_dom_sf
IPR006067 NO2/SO3_Rdtase_4Fe4S_dom
IPR006066 NO2/SO3_Rdtase_FeS/sirohaem_BS
PfamiView protein in Pfam
PF01077 NIR_SIR, 2 hits
PF03460 NIR_SIR_ferr, 2 hits
PRINTSiPR00397 SIROHAEM
SUPFAMiSSF55124 SSF55124, 2 hits
PROSITEiView protein in PROSITE
PS00365 NIR_SIR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q39161-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTSFSLTFTS PLLPSSSTKP KRSVLVAAAQ TTAPAESTAS VDADRLEPRV
60 70 80 90 100
ELKDGFFILK EKFRKGINPQ EKVKIEREPM KLFMENGIEE LAKKSMEELD
110 120 130 140 150
SEKSSKDDID VRLKWLGLFH RRKHQYGKFM MRLKLPNGVT TSAQTRYLAS
160 170 180 190 200
VIRKYGEDGC ADVTTRQNWQ IRGVVLPDVP EILKGLASVG LTSLQSGMDN
210 220 230 240 250
VRNPVGNPIA GIDPEEIVDT RPYTNLLSQF ITANSQGNPD FTNLPRKWNV
260 270 280 290 300
CVVGTHDLYE HPHINDLAYM PANKDGRFGF NLLVGGFFSP KRCEEAIPLD
310 320 330 340 350
AWVPADDVLP LCKAVLEAYR DLGTRGNRQK TRMMWLIDEL GVEGFRTEVE
360 370 380 390 400
KRMPNGKLER GSSEDLVNKQ WERRDYFGVN PQKQEGLSFV GLHVPVGRLQ
410 420 430 440 450
ADDMDELARL ADTYGSGELR LTVEQNIIIP NVETSKTEAL LQEPFLKNRF
460 470 480 490 500
SPEPSILMKG LVACTGSQFC GQAIIETKLR ALKVTEEVER LVSVPRPIRM
510 520 530 540 550
HWTGCPNTCG QVQVADIGFM GCLTRGEEGK PVEGADVYVG GRIGSDSHIG
560 570 580
EIYKKGVRVT ELVPLVAEIL IKEFGAVPRE REENED
Length:586
Mass (Da):65,505
Last modified:November 1, 1996 - v1
Checksum:i6AACF1FE9FF7E1F2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77R → G in AAN31831 (PubMed:14593172).Curated1
Sequence conflicti214P → Q in AAN31830 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14824 Genomic DNA Translation: BAA03561.1
AB006032 mRNA Translation: BAA21672.1
AC006248 Genomic DNA Translation: AAD17406.1
CP002685 Genomic DNA Translation: AEC06424.1
AF360320 mRNA Translation: AAK26030.1
AY045608 mRNA Translation: AAK73966.1
AY093995 mRNA Translation: AAM16256.1
AY142685 mRNA Translation: AAN13223.1
BT000685 mRNA Translation: AAN31830.1
BT000686 mRNA Translation: AAN31831.1
PIRiC84531
RefSeqiNP_179164.1, NM_127123.3
UniGeneiAt.21870

Genome annotation databases

EnsemblPlantsiAT2G15620.1; AT2G15620.1; AT2G15620
GeneIDi816055
GrameneiAT2G15620.1; AT2G15620.1; AT2G15620
KEGGiath:AT2G15620

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14824 Genomic DNA Translation: BAA03561.1
AB006032 mRNA Translation: BAA21672.1
AC006248 Genomic DNA Translation: AAD17406.1
CP002685 Genomic DNA Translation: AEC06424.1
AF360320 mRNA Translation: AAK26030.1
AY045608 mRNA Translation: AAK73966.1
AY093995 mRNA Translation: AAM16256.1
AY142685 mRNA Translation: AAN13223.1
BT000685 mRNA Translation: AAN31830.1
BT000686 mRNA Translation: AAN31831.1
PIRiC84531
RefSeqiNP_179164.1, NM_127123.3
UniGeneiAt.21870

3D structure databases

ProteinModelPortaliQ39161
SMRiQ39161
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi1414, 3 interactors
IntActiQ39161, 2 interactors
STRINGi3702.AT2G15620.1

PTM databases

iPTMnetiQ39161

Proteomic databases

PaxDbiQ39161
PRIDEiQ39161

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G15620.1; AT2G15620.1; AT2G15620
GeneIDi816055
GrameneiAT2G15620.1; AT2G15620.1; AT2G15620
KEGGiath:AT2G15620

Organism-specific databases

AraportiAT2G15620
TAIRilocus:2053654 AT2G15620

Phylogenomic databases

eggNOGiKOG0560 Eukaryota
COG0155 LUCA
HOGENOMiHOG000249408
InParanoidiQ39161
KOiK00366
OMAiCNFALIE
OrthoDBiEOG093604GV
PhylomeDBiQ39161

Enzyme and pathway databases

UniPathwayi
UPA00653

Miscellaneous databases

PROiPR:Q39161

Gene expression databases

ExpressionAtlasiQ39161 baseline and differential
GenevisibleiQ39161 AT

Family and domain databases

InterProiView protein in InterPro
IPR005117 NiRdtase/SiRdtase_haem-b_fer
IPR036136 Nit/Sulf_reduc_fer_like_dom_sf
IPR006067 NO2/SO3_Rdtase_4Fe4S_dom
IPR006066 NO2/SO3_Rdtase_FeS/sirohaem_BS
PfamiView protein in Pfam
PF01077 NIR_SIR, 2 hits
PF03460 NIR_SIR_ferr, 2 hits
PRINTSiPR00397 SIROHAEM
SUPFAMiSSF55124 SSF55124, 2 hits
PROSITEiView protein in PROSITE
PS00365 NIR_SIR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNIR_ARATH
AccessioniPrimary (citable) accession number: Q39161
Secondary accession number(s): Q8H163, Q8H164
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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