NIR1

Functionⁱ

Catalyzes the six-electron reduction of nitrite to ammonium.1 Publication

Catalytic activityⁱ

NH₃ + 2 H₂O + 6 oxidized ferredoxin = nitrite + 6 reduced ferredoxin + 7 H⁺.

Cofactorⁱ

Protein has several cofactor binding sites:

sirohemeNote: Binds 1 siroheme per subunit.
[4Fe-4S] clusterNote: Binds 1 [4Fe-4S] cluster per subunit.

Pathwayⁱ: nitrate reduction (assimilation)

This protein is involved in the pathway nitrate reduction (assimilation), which is part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the pathway nitrate reduction (assimilation) and in Nitrogen metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	464	Iron-sulfur (4Fe-4S)By similarity	1
Metal bindingⁱ	470	Iron-sulfur (4Fe-4S)By similarity	1
Metal bindingⁱ	505	Iron-sulfur (4Fe-4S)By similarity	1
Metal bindingⁱ	509	Iron (siroheme axial ligand)By similarity	1
Metal bindingⁱ	509	Iron-sulfur (4Fe-4S)By similarity	1

GO - Molecular functionⁱ

4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
ferredoxin-nitrite reductase activity Source: UniProtKB-EC
heme binding Source: InterPro
metal ion binding Source: UniProtKB-KW
nitrite reductase (NO-forming) activity
Source: TAIR
Inferred from sequence or structural similarityⁱ
- 7894060

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

nitrate assimilation Source: UniProtKB-UniPathway
response to nitrate
Source: TAIR
Inferred from expression patternⁱ
- 20444232

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Oxidoreductase
Biological process	Electron transport, Nitrate assimilation, Transport
Ligand	4Fe-4S, Heme, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayⁱ	UPA00653

UniPathwayⁱ

UPA00653

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ferredoxin--nitrite reductase, chloroplastic (EC:1.7.7.1) Short name: NiR
Gene namesⁱ	Name:NIR1 Synonyms:NIR Ordered Locus Names:At2g15620 ORF Names:F9O13.17
Organismⁱ	Arabidopsis thaliana (Mouse-ear cress)
Taxonomic identifierⁱ	3702 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
Proteomesⁱ	UP000006548 Componentⁱ: Chromosome 2

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT2G15620
TAIRⁱ	locus:2053654 AT2G15620

Keywords - Cellular componentⁱ

Chloroplast, Plastid

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Transit peptideⁱ	1 – 25	ChloroplastSequence analysisAdd BLAST		25
ChainⁱPRO_0000019703	26 – 586	Ferredoxin--nitrite reductase, chloroplasticAdd BLAST		561

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Cross-linkⁱ	103	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMⁱ

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbⁱ	Q39161
PRIDEⁱ	Q39161

PTM databases

iPTMnetⁱ	Q39161

iPTMnetⁱ

Q39161

Expressionⁱ

Inductionⁱ

By nitrate and by light.1 Publication

Gene expression databases

ExpressionAtlasⁱ	Q39161 baseline and differential
Genevisibleⁱ	Q39161 AT

Interactionⁱ

Subunit structureⁱ

Monomer.

Protein-protein interaction databases

BioGridⁱ	1414, 3 interactors
IntActⁱ	Q39161, 2 interactors
STRINGⁱ	3702.AT2G15620.1

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q39161
SMRⁱ	Q39161
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.Curated

Keywords - Domainⁱ

Repeat, Transit peptide

Phylogenomic databases

eggNOGⁱ	KOG0560 Eukaryota COG0155 LUCA
HOGENOMⁱ	HOG000249408
InParanoidⁱ	Q39161
KEGG Orthology (KO) More...KOⁱ	K00366
OMAⁱ	CNFALIE
Database of Orthologous Groups More...OrthoDBⁱ	EOG093604GV
PhylomeDBⁱ	Q39161

Family and domain databases

InterProⁱ	View protein in InterPro IPR005117 NiRdtase/SiRdtase_haem-b_fer IPR036136 Nit/Sulf_reduc_fer_like_dom_sf IPR006067 NO2/SO3_Rdtase_4Fe4S_dom IPR006066 NO2/SO3_Rdtase_FeS/sirohaem_BS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01077 NIR_SIR, 2 hits PF03460 NIR_SIR_ferr, 2 hits
PRINTSⁱ	PR00397 SIROHAEM
SUPFAMⁱ	SSF55124 SSF55124, 2 hits
PROSITEⁱ	View protein in PROSITE PS00365 NIR_SIR, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q39161-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTSFSLTFTS PLLPSSSTKP KRSVLVAAAQ TTAPAESTAS VDADRLEPRV 
        60         70         80         90        100
ELKDGFFILK EKFRKGINPQ EKVKIEREPM KLFMENGIEE LAKKSMEELD 
       110        120        130        140        150
SEKSSKDDID VRLKWLGLFH RRKHQYGKFM MRLKLPNGVT TSAQTRYLAS 
       160        170        180        190        200
VIRKYGEDGC ADVTTRQNWQ IRGVVLPDVP EILKGLASVG LTSLQSGMDN 
       210        220        230        240        250
VRNPVGNPIA GIDPEEIVDT RPYTNLLSQF ITANSQGNPD FTNLPRKWNV 
       260        270        280        290        300
CVVGTHDLYE HPHINDLAYM PANKDGRFGF NLLVGGFFSP KRCEEAIPLD 
       310        320        330        340        350
AWVPADDVLP LCKAVLEAYR DLGTRGNRQK TRMMWLIDEL GVEGFRTEVE 
       360        370        380        390        400
KRMPNGKLER GSSEDLVNKQ WERRDYFGVN PQKQEGLSFV GLHVPVGRLQ 
       410        420        430        440        450
ADDMDELARL ADTYGSGELR LTVEQNIIIP NVETSKTEAL LQEPFLKNRF 
       460        470        480        490        500
SPEPSILMKG LVACTGSQFC GQAIIETKLR ALKVTEEVER LVSVPRPIRM 
       510        520        530        540        550
HWTGCPNTCG QVQVADIGFM GCLTRGEEGK PVEGADVYVG GRIGSDSHIG 
       560        570        580 
EIYKKGVRVT ELVPLVAEIL IKEFGAVPRE REENED

Length:586

Mass (Da):65,505

Last modified:November 1, 1996 - v1

Checksum:ⁱ6AACF1FE9FF7E1F2

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	77	R → G in AAN31831 (PubMed:14593172).Curated		1
Sequence conflictⁱ	214	P → Q in AAN31830 (PubMed:14593172).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D14824 Genomic DNA Translation: BAA03561.1 AB006032 mRNA Translation: BAA21672.1 AC006248 Genomic DNA Translation: AAD17406.1 CP002685 Genomic DNA Translation: AEC06424.1 AF360320 mRNA Translation: AAK26030.1 AY045608 mRNA Translation: AAK73966.1 AY093995 mRNA Translation: AAM16256.1 AY142685 mRNA Translation: AAN13223.1 BT000685 mRNA Translation: AAN31830.1 BT000686 mRNA Translation: AAN31831.1
PIRⁱ	C84531
NCBI Reference Sequences More...RefSeqⁱ	NP_179164.1, NM_127123.3
UniGeneⁱ	At.21870

Genome annotation databases

EnsemblPlantsⁱ	AT2G15620.1; AT2G15620.1; AT2G15620
GeneIDⁱ	816055
Grameneⁱ	AT2G15620.1; AT2G15620.1; AT2G15620
KEGGⁱ	ath:AT2G15620

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q39161	Ferredoxin--nitrite reductase, chloroplastic (Fragment)		NOCCA		637	UniRef90_Q39161
Nitrite reductase (Fragment)		BRARC		593
Uncharacterized protein		EUTSA		590
Uncharacterized protein		BRARP		589
BnaA07g36300D protein (Fragment)		BRANA		411
+25

Protein	Similar proteins		Species	Score	Length	Source
Q39161	Ferredoxin--nitrite reductase, chloroplastic	)	SPIOL		594	UniRef50_Q39161
Ferredoxin--nitrite reductase, chloroplastic		BETPN		583
Ferredoxin--nitrite reductase, chloroplastic (Fragment)		NOCCA		637
Nitrite reductase (Fragment)		BRARC		593
Uncharacterized protein		EUTSA		590
+262

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D14824 Genomic DNA Translation: BAA03561.1 AB006032 mRNA Translation: BAA21672.1 AC006248 Genomic DNA Translation: AAD17406.1 CP002685 Genomic DNA Translation: AEC06424.1 AF360320 mRNA Translation: AAK26030.1 AY045608 mRNA Translation: AAK73966.1 AY093995 mRNA Translation: AAM16256.1 AY142685 mRNA Translation: AAN13223.1 BT000685 mRNA Translation: AAN31830.1 BT000686 mRNA Translation: AAN31831.1
PIRⁱ	C84531
RefSeqⁱ	NP_179164.1, NM_127123.3
UniGeneⁱ	At.21870

3D structure databases

ProteinModelPortalⁱ	Q39161
SMRⁱ	Q39161
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	1414, 3 interactors
IntActⁱ	Q39161, 2 interactors
STRINGⁱ	3702.AT2G15620.1

PTM databases

iPTMnetⁱ	Q39161

iPTMnetⁱ

Q39161

Proteomic databases

PaxDbⁱ	Q39161
PRIDEⁱ	Q39161

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblPlantsⁱ	AT2G15620.1; AT2G15620.1; AT2G15620
GeneIDⁱ	816055
Grameneⁱ	AT2G15620.1; AT2G15620.1; AT2G15620
KEGGⁱ	ath:AT2G15620

Organism-specific databases

Araportⁱ	AT2G15620
TAIRⁱ	locus:2053654 AT2G15620

Phylogenomic databases

eggNOGⁱ	KOG0560 Eukaryota COG0155 LUCA
HOGENOMⁱ	HOG000249408
InParanoidⁱ	Q39161
KOⁱ	K00366
OMAⁱ	CNFALIE
OrthoDBⁱ	EOG093604GV
PhylomeDBⁱ	Q39161

Enzyme and pathway databases

UniPathwayⁱ	UPA00653

UniPathwayⁱ

UPA00653

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q39161

PROⁱ

PR:Q39161

Gene expression databases

ExpressionAtlasⁱ	Q39161 baseline and differential
Genevisibleⁱ	Q39161 AT

Family and domain databases

InterProⁱ	View protein in InterPro IPR005117 NiRdtase/SiRdtase_haem-b_fer IPR036136 Nit/Sulf_reduc_fer_like_dom_sf IPR006067 NO2/SO3_Rdtase_4Fe4S_dom IPR006066 NO2/SO3_Rdtase_FeS/sirohaem_BS
Pfamⁱ	View protein in Pfam PF01077 NIR_SIR, 2 hits PF03460 NIR_SIR_ferr, 2 hits
PRINTSⁱ	PR00397 SIROHAEM
SUPFAMⁱ	SSF55124 SSF55124, 2 hits
PROSITEⁱ	View protein in PROSITE PS00365 NIR_SIR, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NIR_ARATH
Accessionⁱ	Q39161Primary (citable) accession number: Q39161 Secondary accession number(s): Q8H163, Q8H164
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
	Last sequence update:	November 1, 1996
	Last modified:	April 25, 2018
	This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

UniProtKB

UniParc

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Proteomes

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Supporting data

UniProtKB - Q39161 (NIR_ARATH)

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Ferredoxin--nitrite reductase, chloroplastic

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: nitrate reduction (assimilation)

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Chloroplast

Chloroplast

Extracellular region or secreted

Mitochondrion

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

Pathwayⁱ: nitrate reduction (assimilation)

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ