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Protein

Molybdopterin biosynthesis protein CNX1

Gene

CNX1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.4 Publications

Catalytic activityi

Adenylyl-molybdopterin + molybdate = molybdenum cofactor + AMP.
ATP + molybdopterin = diphosphate + adenylyl-molybdopterin.

Cofactori

Mg2+2 Publications

Activity regulationi

Inhibited by copper and tungsten.1 Publication

Kineticsi

  1. KM=62 µM for ATP for domain G2 Publications
  2. KM=133 µM for Zn2+ for domain E2 Publications
  3. KM=255 µM for Mg2+ for domain E2 Publications

    Pathwayi: molybdopterin biosynthesis

    This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei485AMP1
    Binding sitei486AMP; via amide nitrogen1
    Binding sitei541AMP; via amide nitrogen1
    Binding sitei573Substrate1 Publication1
    Binding sitei600AMP; via carbonyl oxygen1
    Binding sitei600Substrate; via amide nitrogen1 Publication1
    Binding sitei607Substrate1 Publication1

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • molybdenum ion binding Source: TAIR
    • molybdopterin adenylyltransferase activity Source: GO_Central
    • molybdopterin molybdotransferase activity Source: GO_Central
    • nitrate reductase activity Source: CAFA

    GO - Biological processi

    Keywordsi

    Molecular functionMultifunctional enzyme, Transferase
    Biological processMolybdenum cofactor biosynthesis
    LigandATP-binding, Magnesium, Metal-binding, Molybdenum, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.10.1.1 399
    2.7.7.75 399
    ReactomeiR-ATH-947581 Molybdenum cofactor biosynthesis
    UniPathwayi
    UPA00344

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Molybdopterin biosynthesis protein CNX1
    Alternative name(s):
    Molybdenum cofactor biosynthesis enzyme CNX1
    Including the following 2 domains:
    Molybdopterin molybdenumtransferase (EC:2.10.1.1)
    Short name:
    MPT Mo-transferase
    Alternative name(s):
    Domain E
    Molybdopterin adenylyltransferase (EC:2.7.7.75)
    Short name:
    MPT adenylyltransferase
    Alternative name(s):
    Domain G
    Gene namesi
    Name:CNX1
    Ordered Locus Names:At5g20990
    ORF Names:F22D1.6, T10F18.20
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    AraportiAT5G20990
    TAIRilocus:2147157 AT5G20990

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi475S → D: Reduced molybdopterin binding. Loss of adenylation activity. 1 Publication1
    Mutagenesisi485D → S: Reduced molybdopterin binding. Loss of adenylation activity. 1 Publication1
    Mutagenesisi515D → H or N: Almost normal molybdopterin binding. Loss adenylation activity. 2 Publications1
    Mutagenesisi542T → A: Strongly reduced molybdopterin binding. Reduced activity. 1 Publication1
    Mutagenesisi542T → D or N: Loss of molybdopterin binding. Loss of activity. 1 Publication1
    Mutagenesisi542T → S: Reduced molybdopterin binding. No effect on activity. 1 Publication1
    Mutagenesisi542T → V: Strongly reduced molybdopterin binding. No effect on activity. 1 Publication1
    Mutagenesisi547R → E: No effect on molybdopterin binding. Clear reduction in adenylation activity. 1 Publication1
    Mutagenesisi557V → G: Impaired folding. Loss of activity. 1 Publication1
    Mutagenesisi573S → A: Loss of molybdopterin binding. Loss of activity. 1 Publication1
    Mutagenesisi583S → A: No effect on activity. 1 Publication1
    Mutagenesisi597N → L: Loss of molybdopterin binding. Loss of activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001709631 – 670Molybdopterin biosynthesis protein CNX1Add BLAST670

    Proteomic databases

    PaxDbiQ39054
    PRIDEiQ39054

    PTM databases

    iPTMnetiQ39054

    Expressioni

    Gene expression databases

    ExpressionAtlasiQ39054 baseline and differential
    GenevisibleiQ39054 AT

    Interactioni

    Subunit structurei

    The G domain: homotrimer or homohexamer. The E domain: homodimer.4 Publications

    Protein-protein interaction databases

    BioGridi17499, 3 interactors
    STRINGi3702.AT5G20990.1

    Structurei

    Secondary structure

    1670
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliQ39054
    SMRiQ39054
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ39054

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni19 – 439MPT Mo-transferaseAdd BLAST421
    Regioni466 – 620MPT adenylyltransferaseAdd BLAST155
    Regioni542 – 543Substrate binding2

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi8 – 12Poly-Gly5

    Sequence similaritiesi

    In the N-terminal section; belongs to the MoaB/Mog family.Curated
    In the C-terminal section; belongs to the MoeA family.Curated

    Phylogenomic databases

    eggNOGiKOG2371 Eukaryota
    COG0303 LUCA
    COG0521 LUCA
    HOGENOMiHOG000280651
    InParanoidiQ39054
    KOiK15376
    OMAiRPVGCDI
    OrthoDBiEOG093605YJ
    PhylomeDBiQ39054

    Family and domain databases

    CDDicd00887 MoeA, 1 hit
    cd00886 MogA_MoaB, 1 hit
    Gene3Di2.40.340.10, 1 hit
    3.40.980.10, 2 hits
    InterProiView protein in InterPro
    IPR036425 MoaB/Mog-like_dom_sf
    IPR001453 MoaB/Mog_dom
    IPR008284 MoCF_biosynth_CS
    IPR038987 MoeA-like
    IPR005111 MoeA_C_domain_IV
    IPR036688 MoeA_C_domain_IV_sf
    IPR005110 MoeA_linker/N
    IPR036135 MoeA_linker/N_sf
    PANTHERiPTHR10192 PTHR10192, 2 hits
    PfamiView protein in Pfam
    PF00994 MoCF_biosynth, 2 hits
    PF03454 MoeA_C, 1 hit
    PF03453 MoeA_N, 1 hit
    SMARTiView protein in SMART
    SM00852 MoCF_biosynth, 2 hits
    SUPFAMiSSF53218 SSF53218, 2 hits
    SSF63867 SSF63867, 1 hit
    SSF63882 SSF63882, 1 hit
    TIGRFAMsiTIGR00177 molyb_syn, 2 hits
    PROSITEiView protein in PROSITE
    PS01078 MOCF_BIOSYNTHESIS_1, 1 hit
    PS01079 MOCF_BIOSYNTHESIS_2, 1 hit

    Sequence (1+)i

    Sequence statusi: Complete.

    This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.iShow all

    Q39054-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MEGQGCCGGG GGKTEMIPTE EALRIVFGVS KRLPPVIVSL YEALGKVLAE
    60 70 80 90 100
    DIRAPDPLPP YPASVKDGYA VVASDGPGEY PVITESRAGN DGLGVTVTPG
    110 120 130 140 150
    TVAYVTTGGP IPDGADAVVQ VEDTKVIGDV STESKRVKIL IQTKKGTDIR
    160 170 180 190 200
    RVGCDIEKDA TVLTTGERIG ASEIGLLATA GVTMVKVYPM PIVAILSTGD
    210 220 230 240 250
    ELVEPTAGTL GRGQIRDSNR AMLVAAVMQQ QCKVVDLGIV RDDRKELEKV
    260 270 280 290 300
    LDEAVSSGVD IILTSGGVSM GDRDFVKPLL EEKGKVYFSK VLMKPGKPLT
    310 320 330 340 350
    FAEIRAKPTE SMLGKTVLAF GLPGNPVSCL VCFNIFVVPT IRQLAGWTSP
    360 370 380 390 400
    HPLRVRLRLQ EPIKSDPIRP EFHRAIIKWK DNDGSGTPGF VAESTGHQMS
    410 420 430 440 450
    SRLLSMRSAN ALLELPATGN VLSAGSSVSA IIVSDISAFS IDKKASLSEP
    460 470 480 490 500
    GSIRKEKKYD EVPGPEYKVA ILTVSDTVSA GAGPDRSGPR AVSVVDSSSE
    510 520 530 540 550
    KLGGAKVVAT AVVPDEVERI KDILQKWSDV DEMDLILTLG GTGFTPRDVT
    560 570 580 590 600
    PEATKKVIER ETPGLLFVMM QESLKITPFA MLSRSAAGIR GSTLIINMPG
    610 620 630 640 650
    NPNAVAECME ALLPALKHAL KQIKGDKREK HPKHIPHAEA TLPTDTWDQS
    660 670
    YKSAYETGEK KEEAGCSCTH
    Length:670
    Mass (Da):71,279
    Last modified:December 6, 2002 - v2
    Checksum:i4405722BB9C5EF02
    GO

    Computationally mapped potential isoform sequencesi

    There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    P29402CALX1_ARATH
    Calnexin homolog 1
    CNX1 At5g61790, MAC9.11
    530Annotation score:
    A0A1P8BGU1A0A1P8BGU1_ARATH
    Molybdopterin biosynthesis CNX1 pro...
    B73 CHL6, CHLORATE RESISTANT 6, CNX, CNX1, SIR4 B73 CHL6, CHLORATE RESISTANT 6, CNX, CNX1, SIR4, SIRTINOL 4, At5g20990, T10F18.20, T10F18_20
    487Annotation score:
    Q501A2Q501A2_ARATH
    At5g61830
    MAC9.11 MAC9_11, At5g61830
    316Annotation score:

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti113D → V (PubMed:8528286).Curated1
    Sequence conflicti113D → V (Ref. 2) Curated1
    Sequence conflicti134S → A (PubMed:8528286).Curated1
    Sequence conflicti134S → A (Ref. 2) Curated1
    Sequence conflicti653S → L (PubMed:8528286).Curated1
    Sequence conflicti653S → L (Ref. 2) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L47323 mRNA Translation: AAA97413.1
    AJ236870 Genomic DNA Translation: CAB38312.1
    AC069325 Genomic DNA No translation available.
    AF296834 Genomic DNA No translation available.
    CP002688 Genomic DNA Translation: AED92917.1
    RefSeqiNP_197599.1, NM_122108.4
    UniGeneiAt.23035

    Genome annotation databases

    EnsemblPlantsiAT5G20990.1; AT5G20990.1; AT5G20990
    GeneIDi832224
    GrameneiAT5G20990.1; AT5G20990.1; AT5G20990
    KEGGiath:AT5G20990

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L47323 mRNA Translation: AAA97413.1
    AJ236870 Genomic DNA Translation: CAB38312.1
    AC069325 Genomic DNA No translation available.
    AF296834 Genomic DNA No translation available.
    CP002688 Genomic DNA Translation: AED92917.1
    RefSeqiNP_197599.1, NM_122108.4
    UniGeneiAt.23035

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1EAVX-ray2.60A/B/C/D/E/F/G/H462-623[»]
    1O8NX-ray2.80A/B/C462-628[»]
    1O8OX-ray2.70A/B/C462-628[»]
    1O8QX-ray2.60A/B/C/D/E/F/G/H462-628[»]
    1UUXX-ray1.60A462-623[»]
    1UUYX-ray1.45A462-624[»]
    5G2RX-ray2.45A1-451[»]
    5G2SX-ray2.84A1-451[»]
    ProteinModelPortaliQ39054
    SMRiQ39054
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi17499, 3 interactors
    STRINGi3702.AT5G20990.1

    PTM databases

    iPTMnetiQ39054

    Proteomic databases

    PaxDbiQ39054
    PRIDEiQ39054

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT5G20990.1; AT5G20990.1; AT5G20990
    GeneIDi832224
    GrameneiAT5G20990.1; AT5G20990.1; AT5G20990
    KEGGiath:AT5G20990

    Organism-specific databases

    AraportiAT5G20990
    TAIRilocus:2147157 AT5G20990

    Phylogenomic databases

    eggNOGiKOG2371 Eukaryota
    COG0303 LUCA
    COG0521 LUCA
    HOGENOMiHOG000280651
    InParanoidiQ39054
    KOiK15376
    OMAiRPVGCDI
    OrthoDBiEOG093605YJ
    PhylomeDBiQ39054

    Enzyme and pathway databases

    UniPathwayi
    UPA00344

    BRENDAi2.10.1.1 399
    2.7.7.75 399
    ReactomeiR-ATH-947581 Molybdenum cofactor biosynthesis

    Miscellaneous databases

    EvolutionaryTraceiQ39054
    PROiPR:Q39054

    Gene expression databases

    ExpressionAtlasiQ39054 baseline and differential
    GenevisibleiQ39054 AT

    Family and domain databases

    CDDicd00887 MoeA, 1 hit
    cd00886 MogA_MoaB, 1 hit
    Gene3Di2.40.340.10, 1 hit
    3.40.980.10, 2 hits
    InterProiView protein in InterPro
    IPR036425 MoaB/Mog-like_dom_sf
    IPR001453 MoaB/Mog_dom
    IPR008284 MoCF_biosynth_CS
    IPR038987 MoeA-like
    IPR005111 MoeA_C_domain_IV
    IPR036688 MoeA_C_domain_IV_sf
    IPR005110 MoeA_linker/N
    IPR036135 MoeA_linker/N_sf
    PANTHERiPTHR10192 PTHR10192, 2 hits
    PfamiView protein in Pfam
    PF00994 MoCF_biosynth, 2 hits
    PF03454 MoeA_C, 1 hit
    PF03453 MoeA_N, 1 hit
    SMARTiView protein in SMART
    SM00852 MoCF_biosynth, 2 hits
    SUPFAMiSSF53218 SSF53218, 2 hits
    SSF63867 SSF63867, 1 hit
    SSF63882 SSF63882, 1 hit
    TIGRFAMsiTIGR00177 molyb_syn, 2 hits
    PROSITEiView protein in PROSITE
    PS01078 MOCF_BIOSYNTHESIS_1, 1 hit
    PS01079 MOCF_BIOSYNTHESIS_2, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCNX1_ARATH
    AccessioniPrimary (citable) accession number: Q39054
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: December 6, 2002
    Last modified: September 12, 2018
    This is version 153 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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