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Protein

Mitogen-activated protein kinase 5

Gene

MPK5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation (By similarity). Activated by the MAP kinase kinase MKK2. Activated by the MAP kinase kinase MKK6 in vitro.By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72ATPPROSITE-ProRule annotation1
Active sitei169Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi49 – 57ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24 399
ReactomeiR-ATH-198753 ERK/MAPK targets
R-ATH-198765 Signalling to ERK5
R-ATH-202670 ERKs are inactivated
R-ATH-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-ATH-881907 Gastrin-CREB signalling pathway via PKC and MAPK

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 5 (EC:2.7.11.24)
Short name:
AtMPK5
Short name:
MAP kinase 5
Gene namesi
Name:MPK5
Ordered Locus Names:At4g11330
ORF Names:F8L21.120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G11330
TAIRilocus:2128263 AT4G11330

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863141 – 376Mitogen-activated protein kinase 5Add BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei201PhosphothreonineBy similarity1
Modified residuei203PhosphotyrosineBy similarity1
Modified residuei206PhosphothreonineBy similarity1

Post-translational modificationi

Autophosphorylated on threonine and tyrosine residues.By similarity
Dually phosphorylated on Thr-201 and Tyr-203, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ39025
PRIDEiQ39025

Expressioni

Gene expression databases

ExpressionAtlasiQ39025 baseline and differential
GenevisibleiQ39025 AT

Interactioni

Protein-protein interaction databases

BioGridi12034, 1 interactor
IntActiQ39025, 1 interactor
STRINGi3702.AT4G11330.1

Structurei

3D structure databases

ProteinModelPortaliQ39025
SMRiQ39025
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 329Protein kinasePROSITE-ProRule annotationAdd BLAST287

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi201 – 203TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660 Eukaryota
ENOG410XNY0 LUCA
HOGENOMiHOG000233024
InParanoidiQ39025
KOiK04464
OMAiLNHPYIH
OrthoDBiEOG09360BWL
PhylomeDBiQ39025

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q39025-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKEIESATD LGDTNIKGVL VHGGRYFQYN VYGNLFEVSN KYVPPIRPIG
60 70 80 90 100
RGAYGFVCAA VDSETHEEIA IKKIGKAFDN KVDAKRTLRE IKLLRHLEHE
110 120 130 140 150
NVVVIKDIIR PPKKEDFVDV YIVFELMDTD LHQIIRSNQS LNDDHCQYFL
160 170 180 190 200
YQILRGLKYI HSANVLHRDL KPSNLLLNSN CDLKITDFGL ARTTSETEYM
210 220 230 240 250
TEYVVTRWYR APELLLNSSE YTSAIDVWSV GCIFAEIMTR EPLFPGKDYV
260 270 280 290 300
HQLKLITELI GSPDGASLEF LRSANARKYV KELPKFPRQN FSARFPSMNS
310 320 330 340 350
TAIDLLEKML VFDPVKRITV EEALCYPYLS ALHDLNDEPV CSNHFSFHFE
360 370
DPSSTEEEIK ELVWLESVKF NPLPSI
Length:376
Mass (Da):43,207
Last modified:June 6, 2002 - v2
Checksum:i2E8C0E5FC47685DF
GO

Sequence cautioni

The sequence CAB51417 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB81234 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti59A → P in BAA04868 (PubMed:8282107).Curated1
Sequence conflicti276 – 277AR → GG in BAA04868 (PubMed:8282107).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21841 mRNA Translation: BAA04868.1
AL096882 Genomic DNA Translation: CAB51417.1 Sequence problems.
AL161531 Genomic DNA Translation: CAB81234.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE82997.1
AK176361 mRNA Translation: BAD44124.1
PIRiS40471
T13024
RefSeqiNP_567378.4, NM_117204.7
UniGeneiAt.264

Genome annotation databases

EnsemblPlantsiAT4G11330.1; AT4G11330.1; AT4G11330
GeneIDi826735
GrameneiAT4G11330.1; AT4G11330.1; AT4G11330
KEGGiath:AT4G11330

Similar proteinsi

Entry informationi

Entry nameiMPK5_ARATH
AccessioniPrimary (citable) accession number: Q39025
Secondary accession number(s): Q67YV7, Q9SUS8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 6, 2002
Last modified: May 23, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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