UniProtKB - Q38944 (DET2_ARATH)
Steroid 5-alpha-reductase DET2
DET2
Functioni
Involved in a reduction step in the biosynthesis of the plant steroid, brassinolide (BL); acts at the second step in brassinolide biosynthesis in the 5alpha-reduction of (24R)- 24-methylcholest-4-en-3-one, which is further modified to form campestanol. Can use progesterone, testosterone, androstenedione and campestenone as substrate. Catalyzes also the conversion of campest-4-en-3-one (campesta-4-en-3-one, 4-en-3-one) to campest-3-one (campesta-3-one, 3-one), of (22S,24R)-22-hydroxyergost-4-en-3-one (22-hydroxy-campesta-4-en-3-one, 22-OH-4-en-3-one) to (22S,24R)-22-hydroxy-5alpha-ergostan-3-one (22-hydroxy-campesta-3-one, 22-OH-3-one), and of (22R,23R)-22,23-dihydroxy-5alpha-campestan-3-one (22,23,diOH-4-en-3-one) to (22R,23R)-22,23-dihydroxycampest-4-en-3-one (6-deoxo3DT) (PubMed:22822057).
Required for the brassinosteroid- (BR) dependent regulation of seed size and shape as well as embryo development (PubMed:23771896).
8 PublicationsCatalytic activityi
- EC:1.3.1.223 Publications
- This reaction proceeds in the backward1 Publication direction.
- (22S,24R)-22-hydroxy-5α-ergostan-3-one + NADP+ = (22S)-22-hydroxycampest-4-en-3-one + H+ + NADPH1 PublicationThis reaction proceeds in the backward1 Publication direction.
- 3-dehydro-6-deoxoteasterone + NADP+ = (22R,23R)-22,23-dihydroxycampest-4-en-3-one + H+ + NADPH1 PublicationThis reaction proceeds in the forward1 Publication direction.
Activity regulationi
Kineticsi
- KM=15.3 µM for campest-4-en-3-one1 Publication
- KM=3.9 µM for (22S,24R)-22-hydroxyergost-4-en-3-one1 Publication
- KM=8.5 µM for cholest-4-en-3-one1 Publication
- KM=3.0 µM for (22R,23R)-22,23-dihydroxy-campest-4-en-3-one1 Publication
- KM=2.5 µM for testosterone1 Publication
- KM=0.4 µM for progesterone1 Publication
- Vmax=1.4 µmol/min/mg enzyme with campest-4-en-3-one as substrate1 Publication
- Vmax=0.85 µmol/min/mg enzyme with (22S,24R)-22-hydroxyergost-4-en-3-one as substrate1 Publication
- Vmax=1.02 µmol/min/mg enzyme with cholest-4-en-3-one as substrate1 Publication
- Vmax=0.64 µmol/min/mg enzyme with (22R,23R)-22,23-dihydroxy-campest-4-en-3-one as substrate1 Publication
- Vmax=0.2 nmol/min/mg enzyme with testosterone as substrate1 Publication
- Vmax=0.5 nmol/min/mg enzyme with progesterone as substrate1 Publication
pH dependencei
: brassinosteroid biosynthesis Pathwayi
This protein is involved in the pathway brassinosteroid biosynthesis, which is part of Plant hormone biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway brassinosteroid biosynthesis and in Plant hormone biosynthesis.
GO - Molecular functioni
- 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: InterPro
- cholestenone 5-alpha-reductase activity Source: UniProtKB-EC
- oxidoreductase activity Source: GO_Central
- progesterone 5-alpha-reductase activity Source: UniProtKB
- sterol 5-alpha reductase activity Source: TAIR
GO - Biological processi
- brassinosteroid biosynthetic process Source: TAIR
- brassinosteroid homeostasis Source: TAIR
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Brassinosteroid biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis |
Ligand | NADP |
Enzyme and pathway databases
BioCyci | ARA:AT2G38050-MONOMER |
SABIO-RKi | Q38944 |
UniPathwayi | UPA00381 |
Names & Taxonomyi
Protein namesi | Recommended name: Steroid 5-alpha-reductase DET21 Publication (EC:1.3.1.223 Publications)Alternative name(s): (22R,23R)-22,23-dihydroxycampest-4-en-3-one synthase1 Publication (EC:1.3.1.-1 Publication) (22S,24R)-22-hydroxy-5alpha-ergostan-3-one synthase1 Publication (EC:1.3.1.221 Publication) Campest-3-one synthase1 Publication (EC:1.3.1.221 Publication) Protein COMPACT ROSETTE 11 Publication Protein DEETIOLATED 23 Publications Short name: AtDET23 Publications |
Gene namesi | Name:DET23 Publications Synonyms:CRO11 Publication Ordered Locus Names:At2g38050Imported ORF Names:T8P21.4Curated |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G38050 |
TAIRi | locus:2005506, AT2G38050 |
Subcellular locationi
Other locations
- Membrane Sequence analysis; Multi-pass membrane protein Sequence analysis
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 13 – 33 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 51 – 71 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 79 – 99 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 113 – 133 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 148 – 168 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 205 – 225 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 204 | E → K in cro1-2 and det2-1; loss of activity, enhanced sensitivity to abscisic acid (ABA) during seed germination. 6 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000213681 | 1 – 262 | Steroid 5-alpha-reductase DET2Add BLAST | 262 |
Proteomic databases
PaxDbi | Q38944 |
ProteomicsDBi | 224655 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q38944, baseline and differential |
Genevisiblei | Q38944, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 3727, 2 interactors |
IntActi | Q38944, 2 interactors |
STRINGi | 3702.AT2G38050.1 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1638, Eukaryota |
HOGENOMi | CLU_065395_1_0_1 |
InParanoidi | Q38944 |
OMAi | WLEWLGF |
OrthoDBi | 1574389at2759 |
PhylomeDBi | Q38944 |
Family and domain databases
InterProi | View protein in InterPro IPR016636, 3-oxo-5-alpha-steroid_4-DH IPR001104, 3-oxo-5_a-steroid_4-DH_C IPR039357, SRD5A/TECR |
PANTHERi | PTHR10556, PTHR10556, 1 hit |
Pfami | View protein in Pfam PF02544, Steroid_dh, 1 hit |
PIRSFi | PIRSF015596, 5_alpha-SR2, 1 hit |
PROSITEi | View protein in PROSITE PS50244, S5A_REDUCTASE, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MEEIADKTFF RYCLLTLIFA GPPTAVLLKF LQAPYGKHNR TGWGPTVSPP
60 70 80 90 100
IAWFVMESPT LWLTLLLFPF GRHALNPKSL LLFSPYLIHY FHRTIIYPLR
110 120 130 140 150
LFRSSFPAGK NGFPITIAAL AFTFNLLNGY IQARWVSHYK DDYEDGNWFW
160 170 180 190 200
WRFVIGMVVF ITGMYINITS DRTLVRLKKE NRGGYVIPRG GWFELVSCPN
210 220 230 240 250
YFGEAIEWLG WAVMTWSWAG IGFFLYTCSN LFPRARASHK WYIAKFKEEY
260
PKTRKAVIPF VY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 198 | C → R in AAC49264 (PubMed:8602526).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U53860 mRNA Translation: AAC49264.1 CP002685 Genomic DNA Translation: AEC09484.1 AY045926 mRNA Translation: AAK76600.1 AY079337 mRNA Translation: AAL85068.1 |
PIRi | C84800 |
RefSeqi | NP_181340.1, NM_129361.5 |
Genome annotation databases
EnsemblPlantsi | AT2G38050.1; AT2G38050.1; AT2G38050 |
GeneIDi | 818383 |
Gramenei | AT2G38050.1; AT2G38050.1; AT2G38050 |
KEGGi | ath:AT2G38050 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U53860 mRNA Translation: AAC49264.1 CP002685 Genomic DNA Translation: AEC09484.1 AY045926 mRNA Translation: AAK76600.1 AY079337 mRNA Translation: AAL85068.1 |
PIRi | C84800 |
RefSeqi | NP_181340.1, NM_129361.5 |
3D structure databases
AlphaFoldDBi | Q38944 |
SMRi | Q38944 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 3727, 2 interactors |
IntActi | Q38944, 2 interactors |
STRINGi | 3702.AT2G38050.1 |
Proteomic databases
PaxDbi | Q38944 |
ProteomicsDBi | 224655 |
Genome annotation databases
EnsemblPlantsi | AT2G38050.1; AT2G38050.1; AT2G38050 |
GeneIDi | 818383 |
Gramenei | AT2G38050.1; AT2G38050.1; AT2G38050 |
KEGGi | ath:AT2G38050 |
Organism-specific databases
Araporti | AT2G38050 |
TAIRi | locus:2005506, AT2G38050 |
Phylogenomic databases
eggNOGi | KOG1638, Eukaryota |
HOGENOMi | CLU_065395_1_0_1 |
InParanoidi | Q38944 |
OMAi | WLEWLGF |
OrthoDBi | 1574389at2759 |
PhylomeDBi | Q38944 |
Enzyme and pathway databases
UniPathwayi | UPA00381 |
BioCyci | ARA:AT2G38050-MONOMER |
SABIO-RKi | Q38944 |
Miscellaneous databases
PROi | PR:Q38944 |
Gene expression databases
ExpressionAtlasi | Q38944, baseline and differential |
Genevisiblei | Q38944, AT |
Family and domain databases
InterProi | View protein in InterPro IPR016636, 3-oxo-5-alpha-steroid_4-DH IPR001104, 3-oxo-5_a-steroid_4-DH_C IPR039357, SRD5A/TECR |
PANTHERi | PTHR10556, PTHR10556, 1 hit |
Pfami | View protein in Pfam PF02544, Steroid_dh, 1 hit |
PIRSFi | PIRSF015596, 5_alpha-SR2, 1 hit |
PROSITEi | View protein in PROSITE PS50244, S5A_REDUCTASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DET2_ARATH | |
Accessioni | Q38944Primary (citable) accession number: Q38944 Secondary accession number(s): Q9SH83 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2002 |
Last sequence update: | April 16, 2002 | |
Last modified: | May 25, 2022 | |
This is version 140 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families