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Protein

Xyloglucan endotransglucosylase/hydrolase protein 15

Gene

XTH15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues. Has a high XET activity, but little or no XEH activity in vitro. Acceptor preferences are XXXGol > XLLGol = XLFGol > XXLGol > XXFGol.1 Publication

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.1 Publication
Xyloglucan + H2O = xyloglucan oligosaccharides.1 Publication

Kineticsi

KM for xyloglucan as donor substrate is 2.87 mg/ml. KM is quoted in mg/ml, not µM, because XTHs are able to utilise any segment of the polysaccharide chain equally well, not just one site per molecule as with the acceptor.1 Publication
  1. KM=31 µM for XXXGol1 Publication

    pH dependencei

    Optimum pH is 6 for the XET activity.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei102NucleophilePROSITE-ProRule annotation1
    Active sitei106Proton donorPROSITE-ProRule annotation1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosidase, Hydrolase, Transferase
    Biological processCell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciARA:AT4G14130-MONOMER
    SABIO-RKiQ38911

    Protein family/group databases

    CAZyiGH16 Glycoside Hydrolase Family 16

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xyloglucan endotransglucosylase/hydrolase protein 151 Publication (EC:2.4.1.2071 Publication, EC:3.2.1.1511 Publication)
    Short name:
    At-XTH151 Publication
    Short name:
    XTH-151 Publication
    Gene namesi
    Name:XTH151 Publication
    Synonyms:XTR71 Publication
    Ordered Locus Names:At4g14130Imported
    ORF Names:dl3105cImported, FCAALL.173Imported
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    AraportiAT4G14130
    TAIRilocus:2129445 AT4G14130

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Apoplast, Cell wall, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    No visible growth defects, but increased aluminum resistance.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 25Sequence analysisAdd BLAST25
    ChainiPRO_000001181526 – 289Xyloglucan endotransglucosylase/hydrolase protein 15Add BLAST264

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi110N-linked (GlcNAc...) asparagineSequence analysis1

    Post-translational modificationi

    Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ38911
    PRIDEiQ38911

    Expressioni

    Tissue specificityi

    Strongly expressed in roots, hypocotyls and cotyledons. Aslo detected in inflorescence stems and in the carpels and styles in flowers.1 Publication

    Inductioni

    Down-regulated by auxin (PubMed:11673616). Down-regulated by aluminum (PubMed:21285327).2 Publications

    Gene expression databases

    ExpressionAtlasiQ38911 baseline and differential
    GenevisibleiQ38911 AT

    Interactioni

    Protein-protein interaction databases

    BioGridi12348, 1 interactor
    IntActiQ38911, 1 interactor
    STRINGi3702.AT4G14130.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ38911
    SMRiQ38911
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini26 – 216GH16PROSITE-ProRule annotationAdd BLAST191

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG410IVQK Eukaryota
    COG2273 LUCA
    HOGENOMiHOG000236368
    InParanoidiQ38911
    KOiK08235
    OMAiMGSFTHY
    OrthoDBiEOG09360GR5
    PhylomeDBiQ38911

    Family and domain databases

    InterProiView protein in InterPro
    IPR013320 ConA-like_dom_sf
    IPR000757 GH16
    IPR008263 GH16_AS
    IPR010713 XET_C
    IPR016455 XTH
    PfamiView protein in Pfam
    PF00722 Glyco_hydro_16, 1 hit
    PF06955 XET_C, 1 hit
    PIRSFiPIRSF005604 XET, 1 hit
    SUPFAMiSSF49899 SSF49899, 1 hit
    PROSITEiView protein in PROSITE
    PS01034 GH16_1, 1 hit
    PS51762 GH16_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q38911-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGPSSSLTTI VATVLLVTLF GSAYASNFFD EFDLTWGDHR GKIFNGGNML
    60 70 80 90 100
    SLSLDQVSGS GFKSKKEYLF GRIDMQLKLV AGNSAGTVTA YYLSSQGATH
    110 120 130 140 150
    DEIDFEFLGN ETGKPYVLHT NVFAQGKGDR EQQFYLWFDP TKNFHTYSIV
    160 170 180 190 200
    WRPQHIIFLV DNLPIRVFNN AEKLGVPFPK SQPMRIYSSL WNADDWATRG
    210 220 230 240 250
    GLVKTDWSKA PFTAYYRGFN AAACTASSGC DPKFKSSFGD GKLQVATELN
    260 270 280
    AYGRRRLRWV QKYFMIYNYC SDLKRFPRGF PPECKKSRV
    Length:289
    Mass (Da):32,687
    Last modified:November 1, 1996 - v1
    Checksum:i3222ECF640792EF9
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti70F → V (PubMed:9461215).Curated1
    Sequence conflicti70F → V (PubMed:10617198).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U43489 mRNA Translation: AAB18368.1
    Z97335 Genomic DNA Translation: CAB10192.1
    AL161538 Genomic DNA Translation: CAB78455.1
    CP002687 Genomic DNA Translation: AEE83378.1
    AY045865 mRNA Translation: AAK76539.1
    BT020422 mRNA Translation: AAW28549.1
    AY087282 mRNA Translation: AAM64835.1
    PIRiF71402
    RefSeqiNP_193149.2, NM_117490.4
    UniGeneiAt.25124

    Genome annotation databases

    EnsemblPlantsiAT4G14130.1; AT4G14130.1; AT4G14130
    GeneIDi827051
    GrameneiAT4G14130.1; AT4G14130.1; AT4G14130
    KEGGiath:AT4G14130

    Similar proteinsi

    Entry informationi

    Entry nameiXTH15_ARATH
    AccessioniPrimary (citable) accession number: Q38911
    Secondary accession number(s): O23272, Q5M726
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2003
    Last sequence update: November 1, 1996
    Last modified: June 20, 2018
    This is version 137 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

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