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Protein

Inositol-3-phosphate synthase isozyme 2

Gene

IPS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in myo-inositol synthesis.1 Publication

Caution

Was called MIPS1 in PubMed:18603618.Curated

Catalytic activityi

D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate.1 Publication

Cofactori

Kineticsi

kcat is 4.0 min(-1) for D-glucose 6-phosphate. kcat is 3.6 min(-1) for NAD+.
  1. KM=0.45 mM for D-glucose 6-phosphate1 Publication
  2. KM=0.30 µM for NAD+1 Publication

    Pathwayi: myo-inositol biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate.
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Probable inositol 3-phosphate synthase isozyme 3 (IPS3), Inositol-3-phosphate synthase isozyme 2 (IPS2), Inositol-3-phosphate synthase isozyme 1 (IPS1)
    2. Bifunctional phosphatase IMPL2, chloroplastic (HISN7), Phosphatase IMPL1, chloroplastic (IMPL1), Inositol-phosphate phosphatase (VTC4), Inositol-1-monophosphatase (VTC4), Inositol-1-monophosphatase (AXX17_At1g31820), Inositol-1-monophosphatase (AXX17_At3g02150), Inositol-1-monophosphatase (VTC4)
    This subpathway is part of the pathway myo-inositol biosynthesis, which is itself part of Polyol metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate, the pathway myo-inositol biosynthesis and in Polyol metabolism.

    GO - Molecular functioni

    • inositol-3-phosphate synthase activity Source: TAIR

    GO - Biological processi

    Keywordsi

    Molecular functionIsomerase
    Biological processInositol biosynthesis, Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism
    LigandNAD

    Enzyme and pathway databases

    BioCyciARA:AT2G22240-MONOMER
    BRENDAi5.5.1.4 399
    ReactomeiR-ATH-1855183 Synthesis of IP2, IP, and Ins in the cytosol
    SABIO-RKiQ38862
    UniPathwayi
    UPA00823;UER00787

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inositol-3-phosphate synthase isozyme 2 (EC:5.5.1.4)
    Short name:
    AtIPS2
    Short name:
    MIP synthase 2
    Alternative name(s):
    Myo-inositol 1-phosphate synthase 2
    Short name:
    AtMIPS 2
    Short name:
    MI-1-P synthase 2
    Gene namesi
    Name:IPS2
    Synonyms:MIPS2
    Ordered Locus Names:At2g22240
    ORF Names:T26C19.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 2

    Organism-specific databases

    AraportiAT2G22240
    TAIRilocus:2060364 AT2G22240

    Subcellular locationi

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    No spontaneous lesion formation and no significant alteration in the response to abscisic acid. Increased susceptibility to bacterial and fungal pathogens.3 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001951871 – 510Inositol-3-phosphate synthase isozyme 2Add BLAST510

    Proteomic databases

    PaxDbiQ38862
    PRIDEiQ38862

    Expressioni

    Tissue specificityi

    Expressed in siliques, leaves, roots, seed endosperm, but not in embryos. Highest expression in seeds. In leaves, only expressed in hydathodes and vascular tissue.2 Publications

    Gene expression databases

    ExpressionAtlasiQ38862 baseline and differential
    GenevisibleiQ38862 AT

    Interactioni

    Protein-protein interaction databases

    BioGridi2110, 2 interactors
    IntActiQ38862, 2 interactors
    STRINGi3702.AT2G22240.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ38862
    SMRiQ38862
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiKOG0693 Eukaryota
    COG1260 LUCA
    HOGENOMiHOG000013469
    InParanoidiQ38862
    KOiK01858
    OMAiEGWDNID
    OrthoDBiEOG093606K4
    PhylomeDBiQ38862

    Family and domain databases

    InterProiView protein in InterPro
    IPR002587 Myo-inos-1-P_Synthase
    IPR013021 Myo-inos-1-P_Synthase_GAPDH
    IPR036291 NAD(P)-bd_dom_sf
    PANTHERiPTHR11510 PTHR11510, 1 hit
    PfamiView protein in Pfam
    PF01658 Inos-1-P_synth, 1 hit
    PF07994 NAD_binding_5, 1 hit
    PIRSFiPIRSF015578 Myoinos-ppht_syn, 1 hit
    SUPFAMiSSF51735 SSF51735, 2 hits

    Sequence (1+)i

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket
    Note: A number of isoforms are produced. According to EST sequences.

    This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

    Isoform 1 (identifier: Q38862-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide
            10         20         30         40         50
    MFIESFKVES PNVKYTENEI NSVYDYETTE VVHENRNGTY QWVVKPKTVK
    60 70 80 90 100
    YDFKTDTRVP KLGVMLVGWG GNNGSTLTAG VIANKEGISW ATKDKVQQAN
    110 120 130 140 150
    YFGSLTQASS IRVGSYNGEE IYAPFKSLLP MVNPEDVVFG GWDISDMNLA
    160 170 180 190 200
    DAMARARVLD IDLQKQLRPY MENMIPLPGI YDPDFIAANQ GSRANSVIKG
    210 220 230 240 250
    TKKEQVDHII KDMREFKEKN KVDKLVVLWT ANTERYSNVI VGLNDTTENL
    260 270 280 290 300
    LASVEKDESE ISPSTLYAIA CVLEGIPFIN GSPQNTFVPG LIELAISKNC
    310 320 330 340 350
    LIGGDDFKSG QTKMKSVLVD FLVGAGIKPT SIVSYNHLGN NDGMNLSAPQ
    360 370 380 390 400
    TFRSKEISKS NVVDDMVASN GILFEPGEHP DHVVVIKYVP YVADSKRAMD
    410 420 430 440 450
    EYTSEIFMGG RNTIVLHNTC EDSLLAAPII LDLVLLAELS TRIQFKAEGE
    460 470 480 490 500
    GKFHSFHPVA TILSYLTKAP LVPPGTPVVN ALSKQRAMLE NILRACVGLA
    510
    PENNMIMEYK
    Length:510
    Mass (Da):56,337
    Last modified:June 20, 2001 - v2
    Checksum:iBDD58C166E289CE5
    GO

    Computationally mapped potential isoform sequencesi

    There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    F4IIN3F4IIN3_ARATH
    Myo-inositol-1-phosphate synthase 2
    MIPS2 ATIPS2, ATMIPS1, ATMIPS2, MIPS1, At2g22240
    380Annotation score:
    A0A1P8B151A0A1P8B151_ARATH
    Myo-inositol-1-phosphate synthase 2
    MIPS2 ATIPS2, ATMIPS1, ATMIPS2, MIPS1, At2g22240
    464Annotation score:

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti135E → D in AAC49172 (Ref. 1) Curated1
    Sequence conflicti287F → L in AAC49172 (Ref. 1) Curated1
    Sequence conflicti298K → N in AAC49172 (Ref. 1) Curated1
    Sequence conflicti318L → W in AAC49172 (Ref. 1) Curated1
    Sequence conflicti477P → A in AAC49172 (Ref. 1) Curated1
    Sequence conflicti487A → P in AAC49172 (Ref. 1) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U30250 mRNA Translation: AAC49172.1
    AC007168 Genomic DNA Translation: AAD23618.1
    CP002685 Genomic DNA Translation: AEC07280.1
    AY053415 mRNA Translation: AAK96645.1
    AY054202 mRNA Translation: AAL06863.1
    AY143904 mRNA Translation: AAN28843.1
    PIRiD84610
    RefSeqiNP_179812.1, NM_127790.4 [Q38862-1]
    UniGeneiAt.14447
    At.48504

    Genome annotation databases

    EnsemblPlantsiAT2G22240.1; AT2G22240.1; AT2G22240 [Q38862-1]
    GeneIDi816757
    GrameneiAT2G22240.1; AT2G22240.1; AT2G22240 [Q38862-1]
    KEGGiath:AT2G22240

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U30250 mRNA Translation: AAC49172.1
    AC007168 Genomic DNA Translation: AAD23618.1
    CP002685 Genomic DNA Translation: AEC07280.1
    AY053415 mRNA Translation: AAK96645.1
    AY054202 mRNA Translation: AAL06863.1
    AY143904 mRNA Translation: AAN28843.1
    PIRiD84610
    RefSeqiNP_179812.1, NM_127790.4 [Q38862-1]
    UniGeneiAt.14447
    At.48504

    3D structure databases

    ProteinModelPortaliQ38862
    SMRiQ38862
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi2110, 2 interactors
    IntActiQ38862, 2 interactors
    STRINGi3702.AT2G22240.1

    Proteomic databases

    PaxDbiQ38862
    PRIDEiQ38862

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT2G22240.1; AT2G22240.1; AT2G22240 [Q38862-1]
    GeneIDi816757
    GrameneiAT2G22240.1; AT2G22240.1; AT2G22240 [Q38862-1]
    KEGGiath:AT2G22240

    Organism-specific databases

    AraportiAT2G22240
    TAIRilocus:2060364 AT2G22240

    Phylogenomic databases

    eggNOGiKOG0693 Eukaryota
    COG1260 LUCA
    HOGENOMiHOG000013469
    InParanoidiQ38862
    KOiK01858
    OMAiEGWDNID
    OrthoDBiEOG093606K4
    PhylomeDBiQ38862

    Enzyme and pathway databases

    UniPathwayi
    UPA00823;UER00787

    BioCyciARA:AT2G22240-MONOMER
    BRENDAi5.5.1.4 399
    ReactomeiR-ATH-1855183 Synthesis of IP2, IP, and Ins in the cytosol
    SABIO-RKiQ38862

    Miscellaneous databases

    PROiPR:Q38862

    Gene expression databases

    ExpressionAtlasiQ38862 baseline and differential
    GenevisibleiQ38862 AT

    Family and domain databases

    InterProiView protein in InterPro
    IPR002587 Myo-inos-1-P_Synthase
    IPR013021 Myo-inos-1-P_Synthase_GAPDH
    IPR036291 NAD(P)-bd_dom_sf
    PANTHERiPTHR11510 PTHR11510, 1 hit
    PfamiView protein in Pfam
    PF01658 Inos-1-P_synth, 1 hit
    PF07994 NAD_binding_5, 1 hit
    PIRSFiPIRSF015578 Myoinos-ppht_syn, 1 hit
    SUPFAMiSSF51735 SSF51735, 2 hits
    ProtoNetiSearch...

    Entry informationi

    Entry nameiINO2_ARATH
    AccessioniPrimary (citable) accession number: Q38862
    Secondary accession number(s): Q9SIE2
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: June 20, 2001
    Last modified: April 25, 2018
    This is version 144 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
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    Main funding by: National Institutes of Health

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