We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - Q38862 (INO2_ARATH)
Protein
Inositol-3-phosphate synthase isozyme 2
Gene
IPS2
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Involved in myo-inositol synthesis.
1 PublicationCaution
Was called MIPS1 in PubMed:18603618.Curated
Catalytic activityi
- EC:5.5.1.41 Publication
Cofactori
Kineticsi
kcat is 4.0 min(-1) for D-glucose 6-phosphate. kcat is 3.6 min(-1) for NAD+.
- KM=0.45 mM for D-glucose 6-phosphate1 Publication
- KM=0.30 µM for NAD+1 Publication
: myo-inositol biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate. This subpathway is part of the pathway myo-inositol biosynthesis, which is itself part of Polyol metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate, the pathway myo-inositol biosynthesis and in Polyol metabolism.
GO - Molecular functioni
- inositol-3-phosphate synthase activity Source: TAIR
GO - Biological processi
- cellular response to phosphate starvation Source: TAIR
- defense response to bacterium Source: TAIR
- defense response to fungus Source: TAIR
- defense response to virus Source: TAIR
- embryo development ending in seed dormancy Source: TAIR
- inositol biosynthetic process Source: GO_Central
- myo-inositol hexakisphosphate biosynthetic process Source: TAIR
- phospholipid biosynthetic process Source: UniProtKB-KW
- response to auxin Source: TAIR
Keywordsi
Molecular function | Isomerase |
Biological process | Inositol biosynthesis, Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism |
Ligand | NAD |
Enzyme and pathway databases
BioCyci | ARA:AT2G22240-MONOMER |
BRENDAi | 5.5.1.4, 399 |
SABIO-RKi | Q38862 |
UniPathwayi | UPA00823;UER00787 |
Names & Taxonomyi
Protein namesi | Recommended name: Inositol-3-phosphate synthase isozyme 2 (EC:5.5.1.4)Short name: AtIPS2 Short name: MIP synthase 2 Alternative name(s): Myo-inositol 1-phosphate synthase 2 Short name: AtMIPS 2 Short name: MI-1-P synthase 2 |
Gene namesi | Name:IPS2 Synonyms:MIPS2 Ordered Locus Names:At2g22240 ORF Names:T26C19.10 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G22240 |
TAIRi | locus:2060364, AT2G22240 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Other locations
- cytoplasm Source: TAIR
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
No spontaneous lesion formation and no significant alteration in the response to abscisic acid. Increased susceptibility to bacterial and fungal pathogens.3 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000195187 | 1 – 510 | Inositol-3-phosphate synthase isozyme 2Add BLAST | 510 |
Proteomic databases
PaxDbi | Q38862 |
PRIDEi | Q38862 |
Expressioni
Tissue specificityi
Expressed in siliques, leaves, roots, seed endosperm, but not in embryos. Highest expression in seeds. In leaves, only expressed in hydathodes and vascular tissue.2 Publications
Gene expression databases
ExpressionAtlasi | Q38862, baseline and differential |
Genevisiblei | Q38862, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 2110, 2 interactors |
IntActi | Q38862, 2 interactors |
STRINGi | 3702.AT2G22240.1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the myo-inositol 1-phosphate synthase family.Curated
Phylogenomic databases
eggNOGi | KOG0693, Eukaryota |
HOGENOMi | CLU_021486_2_0_1 |
InParanoidi | Q38862 |
OrthoDBi | 451916at2759 |
PhylomeDBi | Q38862 |
Family and domain databases
InterProi | View protein in InterPro IPR002587, Myo-inos-1-P_Synthase IPR013021, Myo-inos-1-P_Synthase_GAPDH IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR11510, PTHR11510, 1 hit |
Pfami | View protein in Pfam PF01658, Inos-1-P_synth, 1 hit PF07994, NAD_binding_5, 1 hit |
PIRSFi | PIRSF015578, Myoinos-ppht_syn, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: A number of isoforms are produced. According to EST sequences.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q38862-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MFIESFKVES PNVKYTENEI NSVYDYETTE VVHENRNGTY QWVVKPKTVK
60 70 80 90 100
YDFKTDTRVP KLGVMLVGWG GNNGSTLTAG VIANKEGISW ATKDKVQQAN
110 120 130 140 150
YFGSLTQASS IRVGSYNGEE IYAPFKSLLP MVNPEDVVFG GWDISDMNLA
160 170 180 190 200
DAMARARVLD IDLQKQLRPY MENMIPLPGI YDPDFIAANQ GSRANSVIKG
210 220 230 240 250
TKKEQVDHII KDMREFKEKN KVDKLVVLWT ANTERYSNVI VGLNDTTENL
260 270 280 290 300
LASVEKDESE ISPSTLYAIA CVLEGIPFIN GSPQNTFVPG LIELAISKNC
310 320 330 340 350
LIGGDDFKSG QTKMKSVLVD FLVGAGIKPT SIVSYNHLGN NDGMNLSAPQ
360 370 380 390 400
TFRSKEISKS NVVDDMVASN GILFEPGEHP DHVVVIKYVP YVADSKRAMD
410 420 430 440 450
EYTSEIFMGG RNTIVLHNTC EDSLLAAPII LDLVLLAELS TRIQFKAEGE
460 470 480 490 500
GKFHSFHPVA TILSYLTKAP LVPPGTPVVN ALSKQRAMLE NILRACVGLA
510
PENNMIMEYK
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF4IIN3 | F4IIN3_ARATH | Inositol-3-phosphate synthase | MIPS2 ATIPS2, ATMIPS1, ATMIPS2, MIPS1, At2g22240 | 380 | Annotation score: | ||
A0A1P8B151 | A0A1P8B151_ARATH | Inositol-3-phosphate synthase | MIPS2 ATIPS2, ATMIPS1, ATMIPS2, MIPS1, At2g22240 | 464 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 135 | E → D in AAC49172 (Ref. 1) Curated | 1 | |
Sequence conflicti | 287 | F → L in AAC49172 (Ref. 1) Curated | 1 | |
Sequence conflicti | 298 | K → N in AAC49172 (Ref. 1) Curated | 1 | |
Sequence conflicti | 318 | L → W in AAC49172 (Ref. 1) Curated | 1 | |
Sequence conflicti | 477 | P → A in AAC49172 (Ref. 1) Curated | 1 | |
Sequence conflicti | 487 | A → P in AAC49172 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U30250 mRNA Translation: AAC49172.1 AC007168 Genomic DNA Translation: AAD23618.1 CP002685 Genomic DNA Translation: AEC07280.1 AY053415 mRNA Translation: AAK96645.1 AY054202 mRNA Translation: AAL06863.1 AY143904 mRNA Translation: AAN28843.1 |
PIRi | D84610 |
RefSeqi | NP_179812.1, NM_127790.4 [Q38862-1] |
Genome annotation databases
EnsemblPlantsi | AT2G22240.1; AT2G22240.1; AT2G22240 [Q38862-1] |
GeneIDi | 816757 |
Gramenei | AT2G22240.1; AT2G22240.1; AT2G22240 [Q38862-1] |
KEGGi | ath:AT2G22240 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U30250 mRNA Translation: AAC49172.1 AC007168 Genomic DNA Translation: AAD23618.1 CP002685 Genomic DNA Translation: AEC07280.1 AY053415 mRNA Translation: AAK96645.1 AY054202 mRNA Translation: AAL06863.1 AY143904 mRNA Translation: AAN28843.1 |
PIRi | D84610 |
RefSeqi | NP_179812.1, NM_127790.4 [Q38862-1] |
3D structure databases
SMRi | Q38862 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 2110, 2 interactors |
IntActi | Q38862, 2 interactors |
STRINGi | 3702.AT2G22240.1 |
Proteomic databases
PaxDbi | Q38862 |
PRIDEi | Q38862 |
Genome annotation databases
EnsemblPlantsi | AT2G22240.1; AT2G22240.1; AT2G22240 [Q38862-1] |
GeneIDi | 816757 |
Gramenei | AT2G22240.1; AT2G22240.1; AT2G22240 [Q38862-1] |
KEGGi | ath:AT2G22240 |
Organism-specific databases
Araporti | AT2G22240 |
TAIRi | locus:2060364, AT2G22240 |
Phylogenomic databases
eggNOGi | KOG0693, Eukaryota |
HOGENOMi | CLU_021486_2_0_1 |
InParanoidi | Q38862 |
OrthoDBi | 451916at2759 |
PhylomeDBi | Q38862 |
Enzyme and pathway databases
UniPathwayi | UPA00823;UER00787 |
BioCyci | ARA:AT2G22240-MONOMER |
BRENDAi | 5.5.1.4, 399 |
SABIO-RKi | Q38862 |
Miscellaneous databases
PROi | PR:Q38862 |
Gene expression databases
ExpressionAtlasi | Q38862, baseline and differential |
Genevisiblei | Q38862, AT |
Family and domain databases
InterProi | View protein in InterPro IPR002587, Myo-inos-1-P_Synthase IPR013021, Myo-inos-1-P_Synthase_GAPDH IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR11510, PTHR11510, 1 hit |
Pfami | View protein in Pfam PF01658, Inos-1-P_synth, 1 hit PF07994, NAD_binding_5, 1 hit |
PIRSFi | PIRSF015578, Myoinos-ppht_syn, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | INO2_ARATH | |
Accessioni | Q38862Primary (citable) accession number: Q38862 Secondary accession number(s): Q9SIE2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 20, 2001 |
Last sequence update: | June 20, 2001 | |
Last modified: | February 23, 2022 | |
This is version 157 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families