UniProtKB - Q38798 (CALX2_ARATH)
Protein
Calnexin homolog 2
Gene
At5g07340
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 34 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 65 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 114 | CarbohydrateBy similarity | 1 | |
Binding sitei | 116 | CarbohydrateBy similarity | 1 | |
Binding sitei | 136 | CarbohydrateBy similarity | 1 | |
Binding sitei | 167 | CarbohydrateBy similarity | 1 | |
Metal bindingi | 384 | CalciumBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: GO_Central
- carbohydrate binding Source: UniProtKB-KW
- unfolded protein binding Source: InterPro
GO - Biological processi
- endoplasmic reticulum unfolded protein response Source: GO_Central
- protein folding Source: GO_Central
Keywordsi
Molecular function | Chaperone |
Ligand | Calcium, Lectin, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Calnexin homolog 2 |
Gene namesi | Ordered Locus Names:At5g07340 ORF Names:T2I1_50 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G07340 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 26 – 468 | LumenalSequence analysisAdd BLAST | 443 | |
Transmembranei | 469 – 489 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 490 – 532 | CytoplasmicSequence analysisAdd BLAST | 43 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | Sequence analysisAdd BLAST | 25 | |
ChainiPRO_0000004203 | 26 – 532 | Calnexin homolog 2Add BLAST | 507 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 110 ↔ 145 | By similarity | ||
Disulfide bondi | 309 ↔ 315 | By similarity | ||
Glycosylationi | 466 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q38798 |
PRIDEi | Q38798 |
ProteomicsDBi | 239190 [Q38798-1] |
PTM databases
SwissPalmi | Q38798 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q38798, baseline and differential |
Genevisiblei | Q38798, AT |
Interactioni
GO - Molecular functioni
- unfolded protein binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 15904, 24 interactors |
IntActi | Q38798, 19 interactors |
STRINGi | 3702.AT5G07340.2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 227 – 238 | 1-1Add BLAST | 12 | |
Repeati | 244 – 255 | 1-2Add BLAST | 12 | |
Repeati | 263 – 274 | 1-3Add BLAST | 12 | |
Repeati | 282 – 293 | 1-4Add BLAST | 12 | |
Repeati | 297 – 307 | 2-1Add BLAST | 11 | |
Repeati | 316 – 326 | 2-2Add BLAST | 11 | |
Repeati | 330 – 340 | 2-3Add BLAST | 11 | |
Repeati | 344 – 354 | 2-4Add BLAST | 11 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 225 – 358 | P domain (Extended arm)By similarityAdd BLAST | 134 | |
Regioni | 227 – 293 | 4 X approximate repeatsAdd BLAST | 67 | |
Regioni | 297 – 354 | 4 X approximate repeatsAdd BLAST | 58 |
Sequence similaritiesi
Belongs to the calreticulin family.Curated
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0675, Eukaryota |
HOGENOMi | CLU_018224_1_0_1 |
InParanoidi | Q38798 |
OMAi | GAIEEKH |
PhylomeDBi | Q38798 |
Family and domain databases
Gene3Di | 2.10.250.10, 1 hit |
InterProi | View protein in InterPro IPR001580, Calret/calnex IPR018124, Calret/calnex_CS IPR009033, Calreticulin/calnexin_P_dom_sf IPR013320, ConA-like_dom_sf |
PANTHERi | PTHR11073, PTHR11073, 1 hit |
Pfami | View protein in Pfam PF00262, Calreticulin, 1 hit |
PRINTSi | PR00626, CALRETICULIN |
SUPFAMi | SSF49899, SSF49899, 1 hit SSF63887, SSF63887, 1 hit |
PROSITEi | View protein in PROSITE PS00803, CALRETICULIN_1, 1 hit PS00804, CALRETICULIN_2, 1 hit PS00805, CALRETICULIN_REPEAT, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: A number of isoforms are produced. According to EST sequences.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q38798-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRERIITFVS LLLVALLSFP SVSYCDDQTI LYESFDEPFD GRWVVSEKAE
60 70 80 90 100
YQGVWKHEKS EGHDDYGLLV SEKAKKYGIV KELDVDEPLN LNEGTVVLQY
110 120 130 140 150
EARFQEGLEC GGAYLKYLRP QEAGWVPQGF DNDSPYSIMF GPDKCGATNK
160 170 180 190 200
VHFILKHKNP KSGEFVEHHL KFPPSVPFDM LSHVYTAVLK SDNEVRILVD
210 220 230 240 250
GEEKKKGNLL SAEDFEPPLI PSKTIPDPED KKPEDWDERA KIPDPNAVKP
260 270 280 290 300
DDWDEDAPME IEDEEAEKPE GWLDDEPVEV EDPEASKPED WDDEEDGEWE
310 320 330 340 350
APKVSNTKCE AAPGCGEWKR PMKRNPAYKG KWSSPLIDNP AYKGIWKPRD
360 370 380 390 400
IPNPDYFELE RPNLEPIAAI GIEIWTMQDG ILFDNILISK DEKVAETYRQ
410 420 430 440 450
STWKPKFDVE KEKQKAEDEA AGEADGLKSY QKKVFDLLYK VADISFLSAY
460 470 480 490 500
KSKIMELIEK AETQPNLTIG VLISIVIVFL SLFFKLIFGG AKAKVEKKKP
510 520 530
ETAAETSTSE AKTEEKAEAV AAPRKRQTRR ES
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF4K6M8 | F4K6M8_ARATH | Calreticulin family protein | At5g07340, T2I1.50, T2I1_50 | 540 | Annotation score: |
Sequence cautioni
The sequence AAA17742 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 30 | I → V in AAA17742 (PubMed:7846171).Curated | 1 | |
Sequence conflicti | 180 | M → K in AAA17742 (PubMed:7846171).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U08315 Genomic DNA Translation: AAA17742.1 Sequence problems. AL163912 Genomic DNA Translation: CAB87923.1 CP002688 Genomic DNA Translation: AED91138.1 BT001999 mRNA Translation: AAN72010.1 BT010385 mRNA Translation: AAQ56828.1 |
PIRi | T49873 |
RefSeqi | NP_196351.1, NM_120816.3 [Q38798-1] |
Genome annotation databases
EnsemblPlantsi | AT5G07340.1; AT5G07340.1; AT5G07340 [Q38798-1] |
GeneIDi | 830625 |
Gramenei | AT5G07340.1; AT5G07340.1; AT5G07340 [Q38798-1] |
KEGGi | ath:AT5G07340 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U08315 Genomic DNA Translation: AAA17742.1 Sequence problems. AL163912 Genomic DNA Translation: CAB87923.1 CP002688 Genomic DNA Translation: AED91138.1 BT001999 mRNA Translation: AAN72010.1 BT010385 mRNA Translation: AAQ56828.1 |
PIRi | T49873 |
RefSeqi | NP_196351.1, NM_120816.3 [Q38798-1] |
3D structure databases
SMRi | Q38798 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 15904, 24 interactors |
IntActi | Q38798, 19 interactors |
STRINGi | 3702.AT5G07340.2 |
PTM databases
SwissPalmi | Q38798 |
Proteomic databases
PaxDbi | Q38798 |
PRIDEi | Q38798 |
ProteomicsDBi | 239190 [Q38798-1] |
Genome annotation databases
EnsemblPlantsi | AT5G07340.1; AT5G07340.1; AT5G07340 [Q38798-1] |
GeneIDi | 830625 |
Gramenei | AT5G07340.1; AT5G07340.1; AT5G07340 [Q38798-1] |
KEGGi | ath:AT5G07340 |
Organism-specific databases
Araporti | AT5G07340 |
Phylogenomic databases
eggNOGi | KOG0675, Eukaryota |
HOGENOMi | CLU_018224_1_0_1 |
InParanoidi | Q38798 |
OMAi | GAIEEKH |
PhylomeDBi | Q38798 |
Miscellaneous databases
PROi | PR:Q38798 |
Gene expression databases
ExpressionAtlasi | Q38798, baseline and differential |
Genevisiblei | Q38798, AT |
Family and domain databases
Gene3Di | 2.10.250.10, 1 hit |
InterProi | View protein in InterPro IPR001580, Calret/calnex IPR018124, Calret/calnex_CS IPR009033, Calreticulin/calnexin_P_dom_sf IPR013320, ConA-like_dom_sf |
PANTHERi | PTHR11073, PTHR11073, 1 hit |
Pfami | View protein in Pfam PF00262, Calreticulin, 1 hit |
PRINTSi | PR00626, CALRETICULIN |
SUPFAMi | SSF49899, SSF49899, 1 hit SSF63887, SSF63887, 1 hit |
PROSITEi | View protein in PROSITE PS00803, CALRETICULIN_1, 1 hit PS00804, CALRETICULIN_2, 1 hit PS00805, CALRETICULIN_REPEAT, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CALX2_ARATH | |
Accessioni | Q38798Primary (citable) accession number: Q38798 Secondary accession number(s): Q9LY26 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | December 13, 2002 | |
Last modified: | December 2, 2020 | |
This is version 163 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families