Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Internal scaffolding protein B

Gene

B

Organism
Enterobacteria phage phiK (Bacteriophage phi-K)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Participates in the assembly of the viral procapsid in the cytoplasm. Forms first a 12S pre-assembly complex with protein H, and F and G pentamers, then twelve 12S complexes are joined by the D protein to form the procapsid. Internal scaffold protein B is released from the procapsid upon genome packaging. Autoproteolytic activity cleaves protein B and probably facilitates its removal through the pores of the procapsid.By similarity

Miscellaneous

Phi KhT, a host-range mutant of phi K, can grow on E.coli C and B, besides K12, and is more thermosensitive than the parental phage phi K.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease
Biological processViral capsid assembly, Viral release from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Internal scaffolding protein B (EC:3.4.-.-By similarity)
Alternative name(s):
Scaffolding protein B
Short name:
GPB
Gene namesi
Name:B
OrganismiEnterobacteria phage phiK (Bacteriophage phi-K)
Taxonomic identifieri10848 [NCBI]
Taxonomic lineageiVirusesssDNA virusesMicroviridaeBullavirinaeAlpha3microvirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000002122 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001648691 – 117Internal scaffolding protein BAdd BLAST117

Post-translational modificationi

The proteolytic cleavage of the internal scaffolding protein B releases the scaffold protein in order to continue virion assembly.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei74 – 75Cleavage; by autolysisBy similarity2
Sitei90 – 91Cleavage; by autolysisBy similarity2
Sitei105 – 106Cleavage; by autolysisBy similarity2

Keywords - PTMi

Autocatalytic cleavage

Proteomic databases

PRIDEiQ38036

Interactioni

Subunit structurei

Component of the procapsid complex composed of 60 copies of the internally located B, 240 copies of the external scaffolding protein D, 60 copies of each of the viral structural proteins F and G proteins, and 12 copies of H.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ38036
SMRiQ38036
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the microviridae B protein family.Curated

Phylogenomic databases

OrthoDBiVOG0900015X

Family and domain databases

Gene3Di4.10.1260.10, 1 hit
InterProiView protein in InterPro
IPR003513 Phage_B
IPR038149 Phage_B_sf
PfamiView protein in Pfam
PF02304 Phage_B, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD012751 Phage_B, 1 hit

Sequencei

Sequence statusi: Complete.

Q38036-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQESINGNLS EERISGAQQP EAWNGAPVNG SPEQQSASGA ESNQLRFQSS
60 70 80 90 100
LSDSERERQK ATDLEHRRAA FARHFGCAPG SEKHVESYSS FDEKDTRVQL
110
AEFYRFNDGH LKKWGYF
Length:117
Mass (Da):13,226
Last modified:November 1, 1997 - v1
Checksum:iABB8292704E13C0E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60323 Genomic DNA Translation: CAA42885.1
RefSeqiNP_043943.1, NC_001730.1

Genome annotation databases

GeneIDi1261193
KEGGivg:1261193

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60323 Genomic DNA Translation: CAA42885.1
RefSeqiNP_043943.1, NC_001730.1

3D structure databases

ProteinModelPortaliQ38036
SMRiQ38036
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ38036

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1261193
KEGGivg:1261193

Phylogenomic databases

OrthoDBiVOG0900015X

Family and domain databases

Gene3Di4.10.1260.10, 1 hit
InterProiView protein in InterPro
IPR003513 Phage_B
IPR038149 Phage_B_sf
PfamiView protein in Pfam
PF02304 Phage_B, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD012751 Phage_B, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSCAFB_BPPHK
AccessioniPrimary (citable) accession number: Q38036
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 18, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again