UniProtKB - Q37373 (NDUS1_ACACA)
Protein
NADH-ubiquinone oxidoreductase 75 kDa subunit
Gene
NAD11
Organism
Acanthamoeba castellanii (Amoeba)
Status
Functioni
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).By similarity
Catalytic activityi
- EC:7.1.1.2
Cofactori
Protein has several cofactor binding sites:- [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
- [4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 36 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 47 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 50 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 64 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 96 | Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 100 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 103 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 109 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 148 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 151 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 154 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 198 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
GO - Biological processi
- ATP synthesis coupled electron transport Source: InterPro
Keywordsi
Molecular function | Oxidoreductase, Translocase |
Biological process | Electron transport, Respiratory chain, Transport |
Ligand | 2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone |
Names & Taxonomyi
Protein namesi | Recommended name: NADH-ubiquinone oxidoreductase 75 kDa subunit (EC:7.1.1.2)Alternative name(s): Complex I-75kD Short name: CI-75kD NADH dehydrogenase subunit 11 |
Gene namesi | Name:NAD11 |
Encoded oni | Mitochondrion |
Organismi | Acanthamoeba castellanii (Amoeba) |
Taxonomic identifieri | 5755 [NCBI] |
Taxonomic lineagei | Eukaryota › Amoebozoa › Discosea › Longamoebia › Centramoebida › Acanthamoebidae › Acanthamoeba |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity
Note: Matrix and cytoplasmic side of the mitochondrial inner membrane.By similarity
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB-SubCell
Other locations
- respirasome Source: UniProtKB-KW
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000118536 | 1 – 675 | NADH-ubiquinone oxidoreductase 75 kDa subunitAdd BLAST | 675 |
Proteomic databases
PRIDEi | Q37373 |
Interactioni
Subunit structurei
Complex I is composed of about 30 different subunits.
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 80 | 2Fe-2S ferredoxin-typeAdd BLAST | 79 | |
Domaini | 80 – 119 | 4Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST | 40 | |
Domaini | 217 – 273 | 4Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST | 57 |
Sequence similaritiesi
Belongs to the complex I 75 kDa subunit family.Curated
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR000283, NADH_UbQ_OxRdtase_75kDa_su_CS IPR010228, NADH_UbQ_OxRdtase_Gsu IPR019574, NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd |
Pfami | View protein in Pfam PF00384, Molybdopterin, 2 hits PF10588, NADH-G_4Fe-4S_3, 1 hit |
SMARTi | View protein in SMART SM00929, NADH-G_4Fe-4S_3, 1 hit |
SUPFAMi | SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR01973, NuoG, 1 hit |
PROSITEi | View protein in PROSITE PS51839, 4FE4S_HC3, 1 hit PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00642, COMPLEX1_75K_2, 1 hit PS00643, COMPLEX1_75K_3, 1 hit |
i Sequence
Sequence statusi: Complete.
Q37373-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKNISFKVND FQYTINNKLT LIQACLKNKV DISRFCFHEK LSIAGNCRMC
60 70 80 90 100
LVEDLKQVKP LASCAINVSN SMNIYTNTLK VKKARESVLE FLLANHPLDC
110 120 130 140 150
PICDQGGECD LQDQSVVFGS DRGRFYEFKR SVEDKDCGPL IKTIMNRCIH
160 170 180 190 200
CTRCVRFSNE VAGVNILGVT GRGSKMEIGF YIENLMRSEL SGNVIDLCPV
210 220 230 240 250
GALTSKPFAF TSRPWELKSY NSIDVLDSLH SNIRVDIRGT KIMRILPRVN
260 270 280 290 300
SELNEDWITD KIRFSYDSFR RQRLYDPMVK ISGSFLKIGW KKAMLFIKKF
310 320 330 340 350
FCNFLGFNHS SFIPLRGYIG DYLDLETIYT FKKFLLLNGS NFFLPSSSYN
360 370 380 390 400
DLTALYSFNT PLTRLDEGDF CILLDVNLRV ELPIVNSRIK QLVSKKMLPV
410 420 430 440 450
FVLGFYSNFN YFVKHISNSS KTLLHVLEGS HWLSAKISKK FSSKPIFLIG
460 470 480 490 500
DSSSLLKGSL IVPLFNFTNV ICDNWNGLNI ISNDSSYLST KEFNLSSSHS
510 520 530 540 550
QNSHLLNFPI NFVLNYDKAV LVDSSAFQIY QGHHGDTNAI NSNLIFPSTS
560 570 580 590 600
FIEKNSFYSN SLAIVQKTKK ILFSPGNSRD DWKILNALID NFGFSYFKVR
610 620 630 640 650
NSFDLVSFLS ESTPFILYKR SFSFKCFGFY EQLVYHFFNY FSVNNNYYIY
660 670
DSITRNSKIM SLCFNKFKMK GYNFF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U12386 Genomic DNA Translation: AAD11824.1 |
PIRi | S53832 |
RefSeqi | NP_042531.1, NC_001637.1 |
Genome annotation databases
GeneIDi | 1734026 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U12386 Genomic DNA Translation: AAD11824.1 |
PIRi | S53832 |
RefSeqi | NP_042531.1, NC_001637.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Proteomic databases
PRIDEi | Q37373 |
Genome annotation databases
GeneIDi | 1734026 |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR000283, NADH_UbQ_OxRdtase_75kDa_su_CS IPR010228, NADH_UbQ_OxRdtase_Gsu IPR019574, NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd |
Pfami | View protein in Pfam PF00384, Molybdopterin, 2 hits PF10588, NADH-G_4Fe-4S_3, 1 hit |
SMARTi | View protein in SMART SM00929, NADH-G_4Fe-4S_3, 1 hit |
SUPFAMi | SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR01973, NuoG, 1 hit |
PROSITEi | View protein in PROSITE PS51839, 4FE4S_HC3, 1 hit PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00642, COMPLEX1_75K_2, 1 hit PS00643, COMPLEX1_75K_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NDUS1_ACACA | |
Accessioni | Q37373Primary (citable) accession number: Q37373 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |