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UniProtKB - Q32KR6 (FDFT_BOVIN)
Protein
Squalene synthase
Gene
FDFT1
Organism
Bos taurus (Bovine)
Status
Functioni
Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene. Proceeds in two distinct steps. In the first half-reaction, two molecules of FPP react to form the stable presqualene diphosphate intermediate (PSQPP), with concomitant release of a proton and a molecule of inorganic diphosphate. In the second half-reaction, PSQPP undergoes heterolysis, isomerization, and reduction with NADPH or NADH to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway.
By similarityCatalytic activityi
- EC:2.5.1.21By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:2.5.1.21By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
Cofactori
Mg2+By similarity
: lanosterol biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.Curated This subpathway is part of the pathway lanosterol biosynthesis, which is itself part of Terpene metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 52 | NADPBy similarity | 1 | |
Binding sitei | 77 | NADPBy similarity | 1 | |
Metal bindingi | 80 | MagnesiumBy similarity | 1 | |
Metal bindingi | 83 | MagnesiumBy similarity | 1 | |
Metal bindingi | 84 | MagnesiumBy similarity | 1 | |
Binding sitei | 218 | NADPBy similarity | 1 | |
Binding sitei | 315 | NADPBy similarity | 1 | |
Binding sitei | 317 | NADPBy similarity | 1 |
GO - Molecular functioni
- farnesyl-diphosphate farnesyltransferase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- squalene synthase activity Source: GO_Central
GO - Biological processi
- cholesterol biosynthetic process Source: GO_Central
- farnesyl diphosphate metabolic process Source: GO_Central
- isoprenoid biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Multifunctional enzyme, Transferase |
Biological process | Cholesterol biosynthesis, Cholesterol metabolism, Isoprene biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism |
Ligand | Magnesium, Metal-binding, NAD, NADP |
Enzyme and pathway databases
UniPathwayi | UPA00767;UER00751 |
Names & Taxonomyi
Protein namesi | Recommended name: Squalene synthase (EC:2.5.1.21By similarity)Short name: SQS Short name: SS Alternative name(s): FPP:FPP farnesyltransferase Farnesyl-diphosphate farnesyltransferase |
Gene namesi | Name:FDFT1 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 284 – 304 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 384 – 404 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000290005 | 1 – 417 | Squalene synthaseAdd BLAST | 417 |
Proteomic databases
PaxDbi | Q32KR6 |
PRIDEi | Q32KR6 |
Family & Domainsi
Sequence similaritiesi
Belongs to the phytoene/squalene synthase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1459, Eukaryota |
InParanoidi | Q32KR6 |
OrthoDBi | 563702at2759 |
Family and domain databases
CDDi | cd00683, Trans_IPPS_HH, 1 hit |
Gene3Di | 1.10.600.10, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR002060, Squ/phyt_synthse IPR006449, Squal_synth-like IPR019845, Squalene/phytoene_synthase_CS IPR044844, Trans_IPPS_euk-type IPR033904, Trans_IPPS_HH |
PANTHERi | PTHR11626, PTHR11626, 1 hit |
Pfami | View protein in Pfam PF00494, SQS_PSY, 1 hit |
SFLDi | SFLDG01018, Squalene/Phytoene_Synthase_Lik, 1 hit |
SUPFAMi | SSF48576, SSF48576, 1 hit |
TIGRFAMsi | TIGR01559, squal_synth, 1 hit |
PROSITEi | View protein in PROSITE PS01044, SQUALEN_PHYTOEN_SYN_1, 1 hit PS01045, SQUALEN_PHYTOEN_SYN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q32KR6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEFVKCLGHP EEFYNLLRFQ MGGRRKVIPK MDQDSLSSSL KTCYKYLNQT
60 70 80 90 100
SRSFAAVIQA LDGEMRHAVC IFYLVLRALD TLEDDMTISI ERKVPLLHNF
110 120 130 140 150
HSYLYEPDWR FTESKEKDRQ VLEDFPTISL EFRNLAEKYQ TVIVDVCQKM
160 170 180 190 200
GFGMAEFLDK RVTSEREWDK YCHYVAGLVG IGLSRLFPAS ELEDPLIGED
210 220 230 240 250
TERANSMGLF LQKTNIIRDY LEDQREGREF WPQETWSKYV KKLGDFAKPE
260 270 280 290 300
NIDLAVQCLN ELITNTLHHI PDVITYLSRL RNQSIFNFCA IPQVMAIATL
310 320 330 340 350
AACYNNQQVF KGVVKIRKGQ AVTLMMDATN MPAVKAIIHQ YMEEIYHRIP
360 370 380 390 400
NSDPCSTKTQ QIISTIRTQN LPNCQLVSRS HYSPIYLSFV MLLAALSWQY
410
LSTLSQVTED YVQTGEH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC109959 mRNA Translation: AAI09960.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC109959 mRNA Translation: AAI09960.1 |
3D structure databases
SMRi | Q32KR6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000016504 |
Proteomic databases
PaxDbi | Q32KR6 |
PRIDEi | Q32KR6 |
Phylogenomic databases
eggNOGi | KOG1459, Eukaryota |
InParanoidi | Q32KR6 |
OrthoDBi | 563702at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00767;UER00751 |
Family and domain databases
CDDi | cd00683, Trans_IPPS_HH, 1 hit |
Gene3Di | 1.10.600.10, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR002060, Squ/phyt_synthse IPR006449, Squal_synth-like IPR019845, Squalene/phytoene_synthase_CS IPR044844, Trans_IPPS_euk-type IPR033904, Trans_IPPS_HH |
PANTHERi | PTHR11626, PTHR11626, 1 hit |
Pfami | View protein in Pfam PF00494, SQS_PSY, 1 hit |
SFLDi | SFLDG01018, Squalene/Phytoene_Synthase_Lik, 1 hit |
SUPFAMi | SSF48576, SSF48576, 1 hit |
TIGRFAMsi | TIGR01559, squal_synth, 1 hit |
PROSITEi | View protein in PROSITE PS01044, SQUALEN_PHYTOEN_SYN_1, 1 hit PS01045, SQUALEN_PHYTOEN_SYN_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FDFT_BOVIN | |
Accessioni | Q32KR6Primary (citable) accession number: Q32KR6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 12, 2007 |
Last sequence update: | December 6, 2005 | |
Last modified: | September 29, 2021 | |
This is version 79 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families