UniProtKB - Q32KD2 (SETB1_DROME)
Protein
Histone-lysine N-methyltransferase eggless
Gene
egg
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 in ovary. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting Su(var)205/HP1 to methylated histones. Plays a central role during oogenesis.1 Publication
Catalytic activityi
- L-lysyl9-[histone H3] + 3 S-adenosyl-L-methionine = 3 H+ + N6,N6,N6-trimethyl-L-lysyl9-[histone H3] + 3 S-adenosyl-L-homocysteineEC:2.1.1.355
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 948 | Zinc 1By similarity | 1 | |
Metal bindingi | 948 | Zinc 2By similarity | 1 | |
Metal bindingi | 950 | Zinc 1By similarity | 1 | |
Metal bindingi | 954 | Zinc 1By similarity | 1 | |
Metal bindingi | 954 | Zinc 3By similarity | 1 | |
Metal bindingi | 960 | Zinc 1By similarity | 1 | |
Metal bindingi | 962 | Zinc 2By similarity | 1 | |
Metal bindingi | 1000 | Zinc 2By similarity | 1 | |
Metal bindingi | 1000 | Zinc 3By similarity | 1 | |
Metal bindingi | 1004 | Zinc 2By similarity | 1 | |
Metal bindingi | 1006 | Zinc 3By similarity | 1 | |
Metal bindingi | 1010 | Zinc 3By similarity | 1 | |
Binding sitei | 1069 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 1071 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 1191 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Metal bindingi | 1197 | Zinc 4By similarity | 1 | |
Metal bindingi | 1250 | Zinc 4By similarity | 1 | |
Metal bindingi | 1252 | Zinc 4By similarity | 1 | |
Metal bindingi | 1257 | Zinc 4By similarity | 1 |
GO - Molecular functioni
- DNA binding Source: InterPro
- histone-lysine N-methyltransferase activity Source: UniProtKB
- histone methyltransferase activity (H3-K9 specific) Source: FlyBase
- zinc ion binding Source: InterPro
GO - Biological processi
- female germ-line stem cell asymmetric division Source: FlyBase
- heterochromatin organization Source: FlyBase
- heterochromatin organization involved in chromatin silencing Source: FlyBase
- histone H3-K9 methylation Source: FlyBase
- multicellular organism development Source: UniProtKB-KW
- negative regulation of gene expression Source: FlyBase
- negative regulation of transcription involved in meiotic cell cycle Source: UniProtKB
- oogenesis Source: FlyBase
- positive regulation of DNA methylation-dependent heterochromatin assembly Source: FlyBase
- regulation of gene expression, epigenetic Source: FlyBase
- regulation of gene silencing Source: FlyBase
Keywordsi
Molecular function | Chromatin regulator, Developmental protein, Methyltransferase, Repressor, Transferase |
Biological process | Differentiation, Oogenesis, Transcription, Transcription regulation |
Ligand | Metal-binding, S-adenosyl-L-methionine, Zinc |
Enzyme and pathway databases
Reactomei | R-DME-3214841, PKMTs methylate histone lysines |
Names & Taxonomyi
Protein namesi | Recommended name: Histone-lysine N-methyltransferase eggless (EC:2.1.1.355)Alternative name(s): SETDB1 homolog |
Gene namesi | Name:egg ORF Names:CG12196 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0086908, egg |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Other locations
- Chromosome 1 Publication
Nucleus
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: FlyBase
- polytene chromosome Source: FlyBase
Keywords - Cellular componenti
Chromosome, NucleusPathology & Biotechi
Disruption phenotypei
Oogenesis arrests at early stages. This arrest is accompanied by reduced proliferation of somatic cells required for egg chamber formation, and by apoptosis in both germ and somatic cell populations.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000281822 | 1 – 1262 | Histone-lysine N-methyltransferase egglessAdd BLAST | 1262 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 215 | Phosphoserine1 Publication | 1 | |
Modified residuei | 217 | Phosphothreonine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q32KD2 |
PRIDEi | Q32KD2 |
PTM databases
iPTMneti | Q32KD2 |
Expressioni
Tissue specificityi
Expressed in ovary (at protein level).1 Publication
Developmental stagei
Present most strongly at early stages of oogenesis, in germ cells in the germarium. Present in germ stem cells and dividing germline cyst cells. Weakly or not expressed in somatic cells at the tip of the germarium, including the terminal filament, inner sheath cells, or cap cells, but it is present at low levels in somatic cells in regions 2 and 3 of the germarium, including the prefollicular cells and the follicle cells of stage 1 egg chambers. Soon after egg chambers budd off the germarium, levels increase in the follicle cells. By mid-oogenesis it decreases in the nurse cells, while levels continued to increase in the follicle cells (at protein level).1 Publication
Gene expression databases
Bgeei | FBgn0086908, Expressed in embryo and 43 other tissues |
Genevisiblei | Q32KD2, DM |
Interactioni
Binary interactionsi
Q32KD2
With | #Exp. | IntAct |
---|---|---|
Pof [Q9W123] | 2 | EBI-140830,EBI-155974 |
Protein-protein interaction databases
BioGRIDi | 63532, 34 interactors |
DIPi | DIP-46503N |
IntActi | Q32KD2, 8 interactors |
STRINGi | 7227.FBpp0111689 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 529 – 602 | Tudor 1Add BLAST | 74 | |
Domaini | 629 – 686 | Tudor 2Add BLAST | 58 | |
Domaini | 818 – 884 | MBDPROSITE-ProRule annotationAdd BLAST | 67 | |
Domaini | 946 – 1018 | Pre-SETPROSITE-ProRule annotationAdd BLAST | 73 | |
Domaini | 1021 – 1237 | SETPROSITE-ProRule annotationAdd BLAST | 217 | |
Domaini | 1246 – 1262 | Post-SETPROSITE-ProRule annotationAdd BLAST | 17 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1031 – 1033 | S-adenosyl-L-methionine bindingBy similarity | 3 | |
Regioni | 1194 – 1195 | S-adenosyl-L-methionine bindingBy similarity | 2 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 353 – 420 | Sequence analysisAdd BLAST | 68 |
Domaini
In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.By similarity
Sequence similaritiesi
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.PROSITE-ProRule annotation
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG1141, Eukaryota |
GeneTreei | ENSGT00940000169356 |
HOGENOMi | CLU_003279_0_1_1 |
InParanoidi | Q32KD2 |
OMAi | TGVRYCN |
OrthoDBi | 183716at2759 |
PhylomeDBi | Q32KD2 |
Family and domain databases
InterProi | View protein in InterPro IPR016177, DNA-bd_dom_sf IPR001739, Methyl_CpG_DNA-bd IPR003616, Post-SET_dom IPR007728, Pre-SET_dom IPR001214, SET_dom IPR041292, Tudor_4 IPR041291, TUDOR_5 |
Pfami | View protein in Pfam PF01429, MBD, 1 hit PF05033, Pre-SET, 1 hit PF00856, SET, 1 hit PF18358, Tudor_4, 1 hit PF18359, TUDOR_5, 1 hit |
SMARTi | View protein in SMART SM00391, MBD, 1 hit SM00468, PreSET, 1 hit SM00317, SET, 1 hit |
SUPFAMi | SSF54171, SSF54171, 1 hit |
PROSITEi | View protein in PROSITE PS50982, MBD, 1 hit PS50868, POST_SET, 1 hit PS50867, PRE_SET, 1 hit PS50280, SET, 1 hit |
i Sequence
Sequence statusi: Complete.
Q32KD2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSGQPTAVDC LESSGSTVED VQETPASREK SYGLPVRKGE NSLESPAEQA
60 70 80 90 100
AKDVEIEELT HSEAIAATGS TRKQCPYGGK APDEPGKLAD ESEDRKGENT
110 120 130 140 150
KAIASSPVLV AVDSDSSVEL IESPVKFSSA NESEKDPPKP DAVNEAAAKE
160 170 180 190 200
AEEMTDSSIS SPTSESFPEK DEKTNKENEQ EPPGMEVDQD VEESISRPAE
210 220 230 240 250
EYKIENTLKG HKRISLTEIE EHKIVDKKDD VLEVELEKGT APKAAEDEKL
260 270 280 290 300
NALLSDGDVF YDKECVNCNC TKLHKQYVLA NMATLNFYQV LRKSSKQQFL
310 320 330 340 350
CMGCHDTAMD LYEEYAGQLM AKQPLLLKDF HQDHADFVAL DSSDEEEEEK
360 370 380 390 400
QPEKSDFSKN KLQLIEDELD DAIKNVLNKV DFTAQLSWSK TILQAKADHL
410 420 430 440 450
ERQFALADVE LEKVQTTADK MHCALYNSCP VAHKHLPTLD IEPSDYVHEV
460 470 480 490 500
PPPGEIVRPP IQLGETYYAV KNKAIASWVS IKVIEFTEST AINGNTMKSY
510 520 530 540 550
KIRYLNTPYQ MIKTVTAKHI AYFEPPPVRL TIGTRVIAYF DGTTLSRGKD
560 570 580 590 600
KGVVQSAFYP GIIAEPLKQA NRYRYLIFYD DGYTQYVPHR DVRLVCQASE
610 620 630 640 650
KVWEDVHAAS RDFIQKYVEK YSVDRPMVQC TRGQSMTTES NGTWLYARVI
660 670 680 690 700
DIDCSLVLMQ FEGDKNHTEW IYRGSLRLGP VFRETQNNMN SSSAQQLRVP
710 720 730 740 750
RRTEPFIRYT KEMESSSKVN QQMRAFARKS SASAQNNALA AASSAATPAG
760 770 780 790 800
GRTNAGGVST SNSASAVRHL NNSTIYVDDE NRPKGHVVYF TAKRNLPPKM
810 820 830 840 850
YKCHECSPNC LFKIVHRLDS YSPLAKPLLS GWERLVMRQK TKKSVVYKGP
860 870 880 890 900
CGKSLRSLAE VHRYLRATEN VLNVDNFDFT PDLKCLAEYS IDPSIVKDTD
910 920 930 940 950
ISKGQEKMAI PLVNYYDNTL PPPCTYAKQR IPTEGVHLNL DEEFLLCCDC
960 970 980 990 1000
EDDCSDKSKC ACWQLTVAGV RYCNPKKPIE EIGYQYKRLH EHVPTGIYEC
1010 1020 1030 1040 1050
NSRCKCKKNC LNRVVQFSLE MKLQVFKTSN RGWGLRCVND IPKGAFICIY
1060 1070 1080 1090 1100
AGHLLTETMA NEGGQDAGDE YFADLDYIEV AEQLKEGYES EVDHSDPDAE
1110 1120 1130 1140 1150
EDNGGPDAED DDDFRPNYHY QRKIKRSSRS GSTQNSSTQS SELDSQERAV
1160 1170 1180 1190 1200
INFNPNADLD ETVRENSVRR LFGKDEAPYI MDAKTTGNLG RYFNHSCSPN
1210 1220 1230 1240 1250
LFVQNVFVDT HDLRFPWVAF FSAAHIRSGT ELTWNYNYEV GVVPGKVLYC
1260
QCGAPNCRLR LL
Sequence cautioni
The sequence AAK93223 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAN71064 differs from that shown. Reason: Erroneous initiation.Curated
The sequence ABC86335 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE013599 Genomic DNA Translation: AAF47268.3 BT023947 mRNA Translation: ABB36451.1 BT024273 mRNA Translation: ABC86335.1 Different initiation. AY051799 mRNA Translation: AAK93223.1 Different initiation. BT001309 mRNA Translation: AAN71064.2 Different initiation. |
RefSeqi | NP_611966.3, NM_138122.5 |
Genome annotation databases
EnsemblMetazoai | FBtr0112777; FBpp0111689; FBgn0086908 |
GeneIDi | 37962 |
KEGGi | dme:Dmel_CG12196 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE013599 Genomic DNA Translation: AAF47268.3 BT023947 mRNA Translation: ABB36451.1 BT024273 mRNA Translation: ABC86335.1 Different initiation. AY051799 mRNA Translation: AAK93223.1 Different initiation. BT001309 mRNA Translation: AAN71064.2 Different initiation. |
RefSeqi | NP_611966.3, NM_138122.5 |
3D structure databases
SMRi | Q32KD2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 63532, 34 interactors |
DIPi | DIP-46503N |
IntActi | Q32KD2, 8 interactors |
STRINGi | 7227.FBpp0111689 |
PTM databases
iPTMneti | Q32KD2 |
Proteomic databases
PaxDbi | Q32KD2 |
PRIDEi | Q32KD2 |
Genome annotation databases
EnsemblMetazoai | FBtr0112777; FBpp0111689; FBgn0086908 |
GeneIDi | 37962 |
KEGGi | dme:Dmel_CG12196 |
Organism-specific databases
CTDi | 37962 |
FlyBasei | FBgn0086908, egg |
Phylogenomic databases
eggNOGi | KOG1141, Eukaryota |
GeneTreei | ENSGT00940000169356 |
HOGENOMi | CLU_003279_0_1_1 |
InParanoidi | Q32KD2 |
OMAi | TGVRYCN |
OrthoDBi | 183716at2759 |
PhylomeDBi | Q32KD2 |
Enzyme and pathway databases
Reactomei | R-DME-3214841, PKMTs methylate histone lysines |
Miscellaneous databases
BioGRID-ORCSi | 37962, 0 hits in 3 CRISPR screens |
GenomeRNAii | 37962 |
PROi | PR:Q32KD2 |
Gene expression databases
Bgeei | FBgn0086908, Expressed in embryo and 43 other tissues |
Genevisiblei | Q32KD2, DM |
Family and domain databases
InterProi | View protein in InterPro IPR016177, DNA-bd_dom_sf IPR001739, Methyl_CpG_DNA-bd IPR003616, Post-SET_dom IPR007728, Pre-SET_dom IPR001214, SET_dom IPR041292, Tudor_4 IPR041291, TUDOR_5 |
Pfami | View protein in Pfam PF01429, MBD, 1 hit PF05033, Pre-SET, 1 hit PF00856, SET, 1 hit PF18358, Tudor_4, 1 hit PF18359, TUDOR_5, 1 hit |
SMARTi | View protein in SMART SM00391, MBD, 1 hit SM00468, PreSET, 1 hit SM00317, SET, 1 hit |
SUPFAMi | SSF54171, SSF54171, 1 hit |
PROSITEi | View protein in PROSITE PS50982, MBD, 1 hit PS50868, POST_SET, 1 hit PS50867, PRE_SET, 1 hit PS50280, SET, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SETB1_DROME | |
Accessioni | Q32KD2Primary (citable) accession number: Q32KD2 Secondary accession number(s): Q29QW7 Q9W110 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2007 |
Last sequence update: | December 6, 2005 | |
Last modified: | April 7, 2021 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families