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Protein

Glutamate 5-kinase

Gene

proB

Organism
Prochlorococcus marinus (strain MIT 9312)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei7ATPUniRule annotation1
Binding sitei47SubstrateUniRule annotation1
Binding sitei134SubstrateUniRule annotation1
Binding sitei146Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi166 – 167ATPUniRule annotation2
Nucleotide bindingi210 – 216ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPMAR74546:G1G51-823-MONOMER
UniPathwayi
UPA00098;UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:PMT9312_0796
OrganismiProchlorococcus marinus (strain MIT 9312)
Taxonomic identifieri74546 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesProchloraceaeProchlorococcus
Proteomesi
  • UP000002715 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002300531 – 360Glutamate 5-kinaseAdd BLAST360

Interactioni

Protein-protein interaction databases

STRINGi74546.PMT9312_0796

Structurei

3D structure databases

ProteinModelPortaliQ31B89
SMRiQ31B89
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini275 – 356PUAUniRule annotationAdd BLAST82

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
HOGENOMiHOG000246369
KOiK00931
OMAiTHEIRFG
OrthoDBiPOG091H00OF

Family and domain databases

Gene3Di2.30.130.10, 1 hit
3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
IPR002478 PUA
IPR015947 PUA-like_sf
IPR036974 PUA_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01472 PUA, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SMARTiView protein in SMART
SM00359 PUA, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
SSF88697 SSF88697, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit
PS50890 PUA, 1 hit

Sequencei

Sequence statusi: Complete.

Q31B89-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKTWVIKIGT SILRGTEETS TEEVIETLCR SFTSFLLKGN KLILVTSGAV
60 70 80 90 100
GLGCQKLNIK TRPNDLSTLQ ATAAVGQVNL MSLYDKIFNK LGHNIAQILI
110 120 130 140 150
TKADFNSRES FNNASKTLKK LIDLNVIPIV NENDTVANEE LKYGDNDTLS
160 170 180 190 200
ALVALAINAN KLILLTDIEN LYSKDPRKNK DAHPIKEVHN SELKEIKNKN
210 220 230 240 250
NQNSNNEWGT GGISTKLISA EIATKGGVEV QLVDGTNKKN LIEIFNDNKI
260 270 280 290 300
GTLFYPLEKP IGNKKSWLSH AIHTVGKITL DDGAFFAINK KGASLLAVGV
310 320 330 340 350
KNVEGNFTVN QAVKIVNTDN KEVAKGLVSI SSDNLRSILN NKDNNNSSII
360
VVHRDVLALS
Length:360
Mass (Da):39,331
Last modified:December 6, 2005 - v1
Checksum:iD9DF7E0AB13E50CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000111 Genomic DNA Translation: ABB49856.1
RefSeqiWP_011376351.1, NC_007577.1

Genome annotation databases

EnsemblBacteriaiABB49856; ABB49856; PMT9312_0796
KEGGipmi:PMT9312_0796

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000111 Genomic DNA Translation: ABB49856.1
RefSeqiWP_011376351.1, NC_007577.1

3D structure databases

ProteinModelPortaliQ31B89
SMRiQ31B89
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi74546.PMT9312_0796

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB49856; ABB49856; PMT9312_0796
KEGGipmi:PMT9312_0796

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
HOGENOMiHOG000246369
KOiK00931
OMAiTHEIRFG
OrthoDBiPOG091H00OF

Enzyme and pathway databases

UniPathwayi
UPA00098;UER00359

BioCyciPMAR74546:G1G51-823-MONOMER

Family and domain databases

Gene3Di2.30.130.10, 1 hit
3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
IPR002478 PUA
IPR015947 PUA-like_sf
IPR036974 PUA_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01472 PUA, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SMARTiView protein in SMART
SM00359 PUA, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
SSF88697 SSF88697, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit
PS50890 PUA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPROB_PROM9
AccessioniPrimary (citable) accession number: Q31B89
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: December 6, 2005
Last modified: October 10, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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