Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamate 5-kinase

Gene

proB

Organism
Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB), Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA), Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14ATPUniRule annotation1
Binding sitei54SubstrateUniRule annotation1
Binding sitei141SubstrateUniRule annotation1
Binding sitei153Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi173 – 174ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNMUL323848:G1GTI-1925-MONOMER
UniPathwayi
UPA00098;UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:Nmul_A1843
OrganismiNitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)
Taxonomic identifieri323848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosospira
Proteomesi
  • UP000002718 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002300501 – 372Glutamate 5-kinaseAdd BLAST372

Proteomic databases

PRIDEiQ2Y7Y3

Interactioni

Protein-protein interaction databases

STRINGi323848.Nmul_A1843

Structurei

3D structure databases

ProteinModelPortaliQ2Y7Y3
SMRiQ2Y7Y3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini280 – 358PUAUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
HOGENOMiHOG000246369
KOiK00931
OMAiTHEIRFG
OrthoDBiPOG091H00OF

Family and domain databases

Gene3Di2.30.130.10, 1 hit
3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
IPR002478 PUA
IPR015947 PUA-like_sf
IPR036974 PUA_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01472 PUA, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SMARTiView protein in SMART
SM00359 PUA, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
SSF88697 SSF88697, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit
PS50890 PUA, 1 hit

Sequencei

Sequence statusi: Complete.

Q2Y7Y3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MESVLKQSRR IVVKVGSSLV TNQGQGLDHS ALAHWAGQIV KLKKTGKEVV
60 70 80 90 100
LVSSGAIAEG MQRLKWKKRP HAVHELQAAA AVGQMGLVQA YETCFRKHEL
110 120 130 140 150
HSAQILLTHE DLSDRKRYLN ARSTLATLLD LNVIPIINEN DTVATDEIRF
160 170 180 190 200
GDNDTLAALV TNLIEADVLV ILTDQRGLFT ADPRKDTQAK LVEQAKAGDA
210 220 230 240 250
DIERMAGGAG SEIGRGGMLT KVLAAKRAAR SGAHTVIASG READVLIRLA
260 270 280 290 300
RGEAIGTQLL AETMTLAARK QWLADHLQIR GNVTLDEGAV EALAVGGKSL
310 320 330 340 350
LPVGVTDVRG NFERGEVVGC LDPHGREIAR GLVNYNAAET RRILRRASDE
360 370
IESLLGYIGE PELIHRDNLV LL
Length:372
Mass (Da):40,227
Last modified:December 20, 2005 - v1
Checksum:i76C914833266D5D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000103 Genomic DNA Translation: ABB75138.1
RefSeqiWP_011381158.1, NZ_FNVK01000004.1

Genome annotation databases

EnsemblBacteriaiABB75138; ABB75138; Nmul_A1843
KEGGinmu:Nmul_A1843

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000103 Genomic DNA Translation: ABB75138.1
RefSeqiWP_011381158.1, NZ_FNVK01000004.1

3D structure databases

ProteinModelPortaliQ2Y7Y3
SMRiQ2Y7Y3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323848.Nmul_A1843

Proteomic databases

PRIDEiQ2Y7Y3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB75138; ABB75138; Nmul_A1843
KEGGinmu:Nmul_A1843

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
HOGENOMiHOG000246369
KOiK00931
OMAiTHEIRFG
OrthoDBiPOG091H00OF

Enzyme and pathway databases

UniPathwayi
UPA00098;UER00359

BioCyciNMUL323848:G1GTI-1925-MONOMER

Family and domain databases

Gene3Di2.30.130.10, 1 hit
3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
IPR002478 PUA
IPR015947 PUA-like_sf
IPR036974 PUA_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01472 PUA, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SMARTiView protein in SMART
SM00359 PUA, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
SSF88697 SSF88697, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit
PS50890 PUA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPROB_NITMU
AccessioniPrimary (citable) accession number: Q2Y7Y3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: December 20, 2005
Last modified: October 10, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again