UniProtKB - Q2XU92 (ACBG2_MOUSE)
Protein
Long-chain-fatty-acid--CoA ligase ACSBG2
Gene
Acsbg2
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the conversion of fatty acids such as long chain and very long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation. Can activate diverse saturated, monosaturated and polyunsaturated fatty acids. Has increased ability to activate oleic and linoleic acid. May play a role in spermatogenesis.By similarity
Catalytic activityi
- EC:6.2.1.3By similarityThis reaction proceeds in the forwardBy similarity direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphateBy similarityEC:6.2.1.15By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 496 | ATPBy similarity | 1 | |
Binding sitei | 511 | ATPBy similarity | 1 | |
Binding sitei | 624 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 227 – 235 | ATPBy similarity | 9 | |
Nucleotide bindingi | 418 – 423 | ATPBy similarity | 6 |
GO - Molecular functioni
- acyl-CoA hydrolase activity Source: MGI
- arachidonate-CoA ligase activity Source: UniProtKB
- ATP binding Source: UniProtKB-KW
- CoA-ligase activity Source: GO_Central
- long-chain fatty acid-CoA ligase activity Source: MGI
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- fatty acid beta-oxidation Source: MGI
- fatty acid metabolic process Source: MGI
- long-chain fatty acid biosynthetic process Source: GO_Central
- multicellular organism development Source: UniProtKB-KW
- spermatogenesis Source: UniProtKB-KW
Keywordsi
Molecular function | Developmental protein, Ligase |
Biological process | Differentiation, Fatty acid metabolism, Lipid metabolism, Spermatogenesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-75876, Synthesis of very long-chain fatty acyl-CoAs |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:3587728, Acsbg2 |
Subcellular locationi
Cytoplasm and Cytosol
Other locations
Cytosol
- cytosol Source: MGI
Mitochondrion
- mitochondrion Source: MGI
Other locations
Keywords - Cellular componenti
Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000315813 | 1 – 667 | Long-chain-fatty-acid--CoA ligase ACSBG2Add BLAST | 667 |
Proteomic databases
PaxDbi | Q2XU92 |
PRIDEi | Q2XU92 |
ProteomicsDBi | 285639 |
PTM databases
iPTMneti | Q2XU92 |
PhosphoSitePlusi | Q2XU92 |
Expressioni
Tissue specificityi
Testis- and brainstem-specific. Expressed in pubertal and adult testis. Enriched in germ cells and Sertoli cells while present at a lower level in Leydig cells. Present in testicular Sertoli cells and large motoneurons in the medulla oblongata and cervical spinal cord (at protein level).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000024207, Expressed in testis and 23 other tissues |
Genevisiblei | Q2XU92, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000042352 |
Miscellaneous databases
RNActi | Q2XU92, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1256, Eukaryota |
GeneTreei | ENSGT00940000155332 |
HOGENOMi | CLU_000022_45_5_1 |
InParanoidi | Q2XU92 |
OMAi | AINECCC |
OrthoDBi | 806831at2759 |
PhylomeDBi | Q2XU92 |
TreeFami | TF354286 |
Family and domain databases
Gene3Di | 3.40.50.12780, 2 hits |
InterProi | View protein in InterPro IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, AMP-dep_Synthh-like_sf |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
i Sequence
Sequence statusi: Complete.
Q2XU92-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTQEKKAEDL ERGTDATSAV PRLWSTHCDG EVLLRLSKHG PGHETPMTIP
60 70 80 90 100
ELFQESAERF SAYPALASKN GKKWDTLTFS QYYEMCRKAA KSLIKLGLQR
110 120 130 140 150
FQCVGILGFN SVEWVVTALG TILAGGLCVG IYATNSAEAC QYVIQQANVS
160 170 180 190 200
ILIVENDQQL QKILLIPPDK METVKAIVQY KLPLMESMAN LYSWNDFMEL
210 220 230 240 250
GNDIPNIQLD RVILSQKANQ CAVILYTSGT TGTPKGVLLS HDNITWTAGA
260 270 280 290 300
MSQEMEINRV SGKQNTIVSY LPLSHIAAQL TDIWIPIKIG ALTFFAQPDA
310 320 330 340 350
LRGTLVYTLQ EVKPTLFMGV PRIWEKMQDT IKENVARSSR LRKKAFAWAK
360 370 380 390 400
MLGLKVNTKR MLGKRDIPMN YRMAKALVFA KVRTSLGLDN CHAFFSSASP
410 420 430 440 450
LSQDVSEFFL SLDIPIGEIY GMSECSGPHT VSNKSVYRVL SCGKVLSGCK
460 470 480 490 500
NMLYNQNKEG VGEVCMWGRH VFMGYLNKEE ATLEALDENG WLHSGDIGRL
510 520 530 540 550
DSHDFLYITG RIKEILITAG GENVSPIPIE TLVKEKIPII SHAMLVGDKA
560 570 580 590 600
KFLCMLLTLK CETDRKSGEP LNKLSVEAKS FCQMLGSQAT TVSDILKSRD
610 620 630 640 650
QVVYTAIQYG IDIVNQQAMS DSHRIRKWII LEKDFSIQGG ELGPTSKLKR
660
SVITQKYKAQ IDSMYLS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ250679 mRNA Translation: ABB54488.1 |
CCDSi | CCDS28916.1 |
RefSeqi | NP_001034203.1, NM_001039114.1 |
Genome annotation databases
Ensembli | ENSMUST00000043062; ENSMUSP00000042352; ENSMUSG00000024207 |
GeneIDi | 328845 |
KEGGi | mmu:328845 |
UCSCi | uc008ddi.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ250679 mRNA Translation: ABB54488.1 |
CCDSi | CCDS28916.1 |
RefSeqi | NP_001034203.1, NM_001039114.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000042352 |
PTM databases
iPTMneti | Q2XU92 |
PhosphoSitePlusi | Q2XU92 |
Proteomic databases
PaxDbi | Q2XU92 |
PRIDEi | Q2XU92 |
ProteomicsDBi | 285639 |
Protocols and materials databases
Antibodypediai | 24085, 136 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000043062; ENSMUSP00000042352; ENSMUSG00000024207 |
GeneIDi | 328845 |
KEGGi | mmu:328845 |
UCSCi | uc008ddi.1, mouse |
Organism-specific databases
CTDi | 81616 |
MGIi | MGI:3587728, Acsbg2 |
Phylogenomic databases
eggNOGi | KOG1256, Eukaryota |
GeneTreei | ENSGT00940000155332 |
HOGENOMi | CLU_000022_45_5_1 |
InParanoidi | Q2XU92 |
OMAi | AINECCC |
OrthoDBi | 806831at2759 |
PhylomeDBi | Q2XU92 |
TreeFami | TF354286 |
Enzyme and pathway databases
Reactomei | R-MMU-75876, Synthesis of very long-chain fatty acyl-CoAs |
Miscellaneous databases
BioGRID-ORCSi | 328845, 1 hit in 52 CRISPR screens |
ChiTaRSi | Acsbg2, mouse |
PROi | PR:Q2XU92 |
RNActi | Q2XU92, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024207, Expressed in testis and 23 other tissues |
Genevisiblei | Q2XU92, MM |
Family and domain databases
Gene3Di | 3.40.50.12780, 2 hits |
InterProi | View protein in InterPro IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, AMP-dep_Synthh-like_sf |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACBG2_MOUSE | |
Accessioni | Q2XU92Primary (citable) accession number: Q2XU92 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | December 20, 2005 | |
Last modified: | April 7, 2021 | |
This is version 110 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families