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UniProtKB - Q2VUH7 (DIXC1_RAT)
Protein
Dixin
Gene
Dixdc1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Positive effector of the Wnt signaling pathway; activates WNT3A signaling via DVL2. Regulates JNK activation by AXIN1 and DVL2.
By similarityGO - Molecular functioni
- actin binding Source: UniProtKB-KW
- gamma-tubulin binding Source: UniProtKB
- mitogen-activated protein kinase kinase kinase binding Source: RGD
- protein domain specific binding Source: RGD
GO - Biological processi
- canonical Wnt signaling pathway Source: UniProtKB
- cell proliferation in forebrain Source: RGD
- cerebellar cortex development Source: RGD
- cerebral cortex cell migration Source: RGD
- cerebral cortex radially oriented cell migration Source: RGD
- forebrain ventricular zone progenitor cell division Source: RGD
- negative regulation of neuron differentiation Source: RGD
- positive regulation of axonogenesis Source: RGD
- positive regulation of JNK cascade Source: RGD
- positive regulation of Wnt signaling pathway Source: RGD
- regulation of actin cytoskeleton organization Source: RGD
- regulation of microtubule cytoskeleton organization Source: RGD
Keywordsi
Molecular function | Actin-binding, Developmental protein |
Biological process | Wnt signaling pathway |
Names & Taxonomyi
Protein namesi | Recommended name: DixinAlternative name(s): Coiled-coil protein DIX1 Short name: Coiled-coil-DIX1 DIX domain-containing protein 1 |
Gene namesi | Name:Dixdc1 Synonyms:Ccd1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1309902, Dixdc1 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytoskeleton
- stress fiber By similarity
Other locations
- focal adhesion By similarity
Note: Colocalizes with gamma-tubulin at the centrosome, both during interphase and mitosis. Associated with actin stress fiber at the filament ends.By similarity
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Cytosol
- cytosol Source: UniProtKB
Other locations
- axon terminus Source: RGD
- cytoplasm Source: RGD
- focal adhesion Source: UniProtKB-SubCell
- neuronal cell body Source: RGD
- protein-containing complex Source: RGD
Keywords - Cellular componenti
Cell junction, Cytoplasm, CytoskeletonPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000287225 | 1 – 684 | DixinAdd BLAST | 684 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 186 | PhosphoserineBy similarity | 1 | |
Modified residuei | 231 | PhosphoserineBy similarity | 1 | |
Modified residuei | 592 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylated on tyrosine and serine residues.By similarity
Polyubiquitinated, leading to its proteasomal degradation. WNT3A signaling increases DIXDC1 protein levels by inhibiting its ubiquitination and subsequent degradation (By similarity).By similarity
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | Q2VUH7 |
PTM databases
iPTMneti | Q2VUH7 |
PhosphoSitePlusi | Q2VUH7 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000010260, Expressed in cerebellum and 21 other tissues |
ExpressionAtlasi | Q2VUH7, baseline and differential |
Genevisiblei | Q2VUH7, RN |
Interactioni
Subunit structurei
May bind filamentous actin.
Interacts with the complex composed of DVL2 and Rac.
Interacts with AXIN1; competes with MAP3K1.
Interacts with MAP3K4 preventing MAP3K4 interaction with AXIN1. Directly interacts (via DIX domain) with DVL2 (via DIX domain).
Interacts with gamma-tubulin.
By similarityGO - Molecular functioni
- actin binding Source: UniProtKB-KW
- gamma-tubulin binding Source: UniProtKB
- mitogen-activated protein kinase kinase kinase binding Source: RGD
- protein domain specific binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000037500 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 20 – 127 | Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST | 108 | |
Domaini | 602 – 682 | DIXPROSITE-ProRule annotationAdd BLAST | 81 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 127 – 300 | Actin-bindingBy similarityAdd BLAST | 174 | |
Regioni | 207 – 235 | DisorderedSequence analysisAdd BLAST | 29 | |
Regioni | 560 – 597 | DisorderedSequence analysisAdd BLAST | 38 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 277 – 308 | Sequence analysisAdd BLAST | 32 | |
Coiled coili | 336 – 461 | Sequence analysisAdd BLAST | 126 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 572 – 597 | Polar residuesSequence analysisAdd BLAST | 26 |
Domaini
The coiled-coil domain mediates interaction with MAP3K4 and inhibition of AXIN1-mediated JNK activation through MAP3K4.By similarity
The DIX domain mediates interaction with AXIN1 and inhibition of AXIN1-mediated JNK activation through MAP3K1. Mediates interaction with DVL2; this interaction is required for activation of Wnt signaling (By similarity).By similarity
Sequence similaritiesi
Belongs to the DIXDC1 family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502RD5G, Eukaryota |
GeneTreei | ENSGT00950000182903 |
HOGENOMi | CLU_025678_0_0_1 |
InParanoidi | Q2VUH7 |
OMAi | AHFKPTA |
OrthoDBi | 949210at2759 |
PhylomeDBi | Q2VUH7 |
Family and domain databases
CDDi | cd00014, CH, 1 hit |
Gene3Di | 1.10.418.10, 1 hit 2.40.240.130, 1 hit |
InterProi | View protein in InterPro IPR001715, CH-domain IPR036872, CH_dom_sf IPR001158, DIX IPR038207, DIX_dom_sf IPR029800, Dixin IPR015506, Dsh/Dvl-rel IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR10878, PTHR10878, 1 hit PTHR10878:SF22, PTHR10878:SF22, 1 hit |
Pfami | View protein in Pfam PF00307, CH, 1 hit PF00778, DIX, 1 hit |
SMARTi | View protein in SMART SM00033, CH, 1 hit SM00021, DAX, 1 hit |
SUPFAMi | SSF47576, SSF47576, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50021, CH, 1 hit PS50841, DIX, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q2VUH7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLACLTRGNL LDVLQEGFNE QQLQAYVAWV NAQLKKRPSV KPVQDLRQDL
60 70 80 90 100
RDGVILAYLI EIVAGEKLSG VQLSPSNQQE MKSNVERVLQ FVASKNIRMH
110 120 130 140 150
QTSAKDIVEG NLKSIMRLVL ALAAHFKPGS SRTVSQGRDS RTSVQSHQPH
160 170 180 190 200
CATAVAQGAA AALADVCHDV SRSGRDVFRY RQRNASVDEE IENPYWSVRA
210 220 230 240 250
LVQQYEGQQR SPSESSCSSL TSPSPIHSAK SESIITQSEE KADFVIIPSE
260 270 280 290 300
GIENRTDETG SPLSRDWRPG SPGTYLEATW EEQLLEQQEH LEKEMEEAKK
310 320 330 340 350
MISGLQALLL NGSLPEDEQE RPVALCEPGV NPEEQLIIIR SRLDQSMEEN
360 370 380 390 400
QDLKKELLKC KQEARNLQGI KDALQQRLTQ QDTSVLQLKQ ELLRANMDKD
410 420 430 440 450
ELHNQNVDLQ RKLDERNRLL GEYKKDLGQK DRLLQQQQAK LEDALRKLSD
460 470 480 490 500
ASYQQVDLER ELEQKDVLLA HRVKGDTDEV TNYNSHSSQR NGFVLPVAGR
510 520 530 540 550
AATTATHRGP QSSDLQLVRD ALRSLRNSFS GHDPQHHTID SLEQGISSLI
560 570 580 590 600
ERLHVIETQK KQERKVRGRS PRNQASSEYR ASWPPNSTLP HSQSSPAVSN
610 620 630 640 650
TCTKVLYFTD RSLTPFMVNI PKRLGEVTLK DFKAAIDREG SHRYHFKALD
660 670 680
PEFGTVKEEV FHDDDAIPGW EGKIVAWVED HREN
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2K662 | A0A0G2K662_RAT | Dixin | Dixdc1 | 683 | Annotation score: |
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY770507 mRNA Translation: AAX76925.1 Sequence problems. AC094189 Genomic DNA No translation available. AC132668 Genomic DNA No translation available. |
RefSeqi | NP_001032743.1, NM_001037654.1 XP_006243104.1, XM_006243042.3 |
Genome annotation databases
Ensembli | ENSRNOT00000039045; ENSRNOP00000037500; ENSRNOG00000010260 |
GeneIDi | 363062 |
KEGGi | rno:363062 |
UCSCi | RGD:1309902, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY770507 mRNA Translation: AAX76925.1 Sequence problems. AC094189 Genomic DNA No translation available. AC132668 Genomic DNA No translation available. |
RefSeqi | NP_001032743.1, NM_001037654.1 XP_006243104.1, XM_006243042.3 |
3D structure databases
SMRi | Q2VUH7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000037500 |
PTM databases
iPTMneti | Q2VUH7 |
PhosphoSitePlusi | Q2VUH7 |
Proteomic databases
PaxDbi | Q2VUH7 |
Genome annotation databases
Ensembli | ENSRNOT00000039045; ENSRNOP00000037500; ENSRNOG00000010260 |
GeneIDi | 363062 |
KEGGi | rno:363062 |
UCSCi | RGD:1309902, rat |
Organism-specific databases
CTDi | 85458 |
RGDi | 1309902, Dixdc1 |
Phylogenomic databases
eggNOGi | ENOG502RD5G, Eukaryota |
GeneTreei | ENSGT00950000182903 |
HOGENOMi | CLU_025678_0_0_1 |
InParanoidi | Q2VUH7 |
OMAi | AHFKPTA |
OrthoDBi | 949210at2759 |
PhylomeDBi | Q2VUH7 |
Miscellaneous databases
PROi | PR:Q2VUH7 |
Gene expression databases
Bgeei | ENSRNOG00000010260, Expressed in cerebellum and 21 other tissues |
ExpressionAtlasi | Q2VUH7, baseline and differential |
Genevisiblei | Q2VUH7, RN |
Family and domain databases
CDDi | cd00014, CH, 1 hit |
Gene3Di | 1.10.418.10, 1 hit 2.40.240.130, 1 hit |
InterProi | View protein in InterPro IPR001715, CH-domain IPR036872, CH_dom_sf IPR001158, DIX IPR038207, DIX_dom_sf IPR029800, Dixin IPR015506, Dsh/Dvl-rel IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR10878, PTHR10878, 1 hit PTHR10878:SF22, PTHR10878:SF22, 1 hit |
Pfami | View protein in Pfam PF00307, CH, 1 hit PF00778, DIX, 1 hit |
SMARTi | View protein in SMART SM00033, CH, 1 hit SM00021, DAX, 1 hit |
SUPFAMi | SSF47576, SSF47576, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50021, CH, 1 hit PS50841, DIX, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DIXC1_RAT | |
Accessioni | Q2VUH7Primary (citable) accession number: Q2VUH7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 15, 2007 |
Last sequence update: | September 29, 2021 | |
Last modified: | February 23, 2022 | |
This is version 106 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families