UniProtKB - Q2VPU4 (MLXIP_MOUSE)
MLX-interacting protein
Mlxip
Functioni
Binds DNA as a heterodimer with MLX and activates transcription. Binds to the canonical E box sequence 5'-CACGTG-3'. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.
By similarity2 PublicationsGO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
GO - Biological processi
- nucleocytoplasmic transport Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: MLX-interacting proteinAlternative name(s): Transcriptional activator MondoA |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2141183, Mlxip |
Subcellular locationi
Mitochondrion
- Mitochondrion outer membrane 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Nucleus
- Nucleus PROSITE-ProRule annotation1 Publication
Note: Predominantly cytoplasmic but shuttles between cytoplasm and nucleus when associated with MLX. Also associates with the outer mitochondrial membrane and may shuttle between the outer mitochondrial membrane and the nucleus.By similarity1 Publication
Mitochondrion
- mitochondrial outer membrane Source: UniProtKB-SubCell
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000298764 | 2 – 917 | MLX-interacting proteinAdd BLAST | 916 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 9 | PhosphoserineBy similarity | 1 | |
Modified residuei | 33 | PhosphoserineBy similarity | 1 | |
Modified residuei | 39 | PhosphoserineCombined sources | 1 | |
Modified residuei | 667 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
MaxQBi | Q2VPU4 |
PaxDbi | Q2VPU4 |
PRIDEi | Q2VPU4 |
ProteomicsDBi | 295682 [Q2VPU4-1] 295683 [Q2VPU4-2] |
PTM databases
iPTMneti | Q2VPU4 |
PhosphoSitePlusi | Q2VPU4 |
Expressioni
Developmental stagei
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer with MLX.
1 PublicationGO - Molecular functioni
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 228948, 3 interactors |
STRINGi | 10090.ENSMUSP00000064943 |
Miscellaneous databases
RNActi | Q2VPU4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 717 – 767 | bHLHPROSITE-ProRule annotationAdd BLAST | 51 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 72 | DisorderedSequence analysisAdd BLAST | 72 | |
Regioni | 73 – 327 | Required for cytoplasmic localizationBy similarityAdd BLAST | 255 | |
Regioni | 322 – 445 | Transactivation domain1 PublicationAdd BLAST | 124 | |
Regioni | 347 – 402 | DisorderedSequence analysisAdd BLAST | 56 | |
Regioni | 632 – 711 | DisorderedSequence analysisAdd BLAST | 80 | |
Regioni | 767 – 788 | Leucine-zipperAdd BLAST | 22 | |
Regioni | 830 – 879 | Mediates heterotypic interactions between MLXIP and MLX and is required for cytoplasmic localizationBy similarityAdd BLAST | 50 | |
Regioni | 897 – 917 | DisorderedSequence analysisAdd BLAST | 21 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 358 – 402 | Polar residuesSequence analysisAdd BLAST | 45 | |
Compositional biasi | 632 – 657 | Polar residuesSequence analysisAdd BLAST | 26 | |
Compositional biasi | 666 – 711 | Polar residuesSequence analysisAdd BLAST | 46 |
Phylogenomic databases
eggNOGi | KOG3582, Eukaryota |
InParanoidi | Q2VPU4 |
OrthoDBi | 388166at2759 |
PhylomeDBi | Q2VPU4 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR032648, MLXIP |
PANTHERi | PTHR15741:SF23, PTHR15741:SF23, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAADVFMCSP RRPRSRGRSV LLKPQVPEDD DDSDTDEPSP PPPSGVATSA
60 70 80 90 100
RAHASAAPLP PRAGPGREEP PRRQQIIHSG HFMVSSPHRE HPPKKGYDFD
110 120 130 140 150
TVNKQTCQTY SFGKTSSCHL SIDASLTKLF ECMTLAYSGK LVSPKWKNFK
160 170 180 190 200
GLKLQWRDKI RLNNAIWRAW YMQYLEKRRN PVCHFVTPLD GSVDVDEHRR
210 220 230 240 250
PEAITTEGKY WKSRIEIVIR EYHKWRTYFK KRLQQHKDED LSSLAQDDDM
260 270 280 290 300
LYWHKHGDGW KTPVPMEEDS LLDTDMLMSE FSDTLFSTLS SHQPVAWPNP
310 320 330 340 350
REIAHLGNAD MIQPGLIPLQ PNLDFMDTFE PFQDLFSSSR SIFGSMLPPP
360 370 380 390 400
SSLPAADPSS PPSQGNILPN TALPPASLPN SLITSSAAPS LDPTEGQGCE
410 420 430 440 450
RTSQTVDPFI QPADFGPSEP PLSVPQPFLP VFTMTLLSPG PAPAPVPTAL
460 470 480 490 500
PLVPSPAPTL NPPTPPAFLQ PQKFAGVSKS TPVITHTASA TLTHDASATT
510 520 530 540 550
FSQNQGLVIT AHHPTPSSSP CALALSPVPQ PPAVGPPQPH LTFIHPKPVS
560 570 580 590 600
LTGVRHKQPP KIVPAPKPEP VSLVLKNACI APAAFSGQPQ KVIMTSAPLK
610 620 630 640 650
REGILASTVS PSNVVIASAA ITRASGVTEF LSHSTSSQPS PVSRLFSPST
660 670 680 690 700
VQDSLVKGEQ VSLHGGSPQV PATGSSRDCP NSGQASPCPS EQSPSPQSPQ
710 720 730 740 750
NNCSGKSTDP KNVAALKNRQ KHISAEQKRR FNIRMGFNTL NSLISNNSKQ
760 770 780 790 800
TSHAITLQKT MEYITKLQQE RMQMQEEARR LREEIEELNT TIISCQQLLP
810 820 830 840 850
ATGVPVNCRQ LDHMRDMFDE YVKSRTLQNW KFWIFSMIIK PLFESFKGMV
860 870 880 890 900
STSSLEEFHR TALSWLDQHC SLPVLRPMVL STLRQLSTTT SILTDPSQLP
910
EQASEAVTRM GKRSGES
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG5E8D8 | G5E8D8_MOUSE | MLX-interacting protein | Mlxip | 917 | Annotation score: | ||
F6UPM1 | F6UPM1_MOUSE | MLX-interacting protein | Mlxip | 241 | Annotation score: | ||
B2RQ56 | B2RQ56_MOUSE | MLX-interacting protein | Mlxip | 614 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 179 | R → K in BAC33069 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_052505 | 583 – 614 | AAFSG…SPSNV → GELVGGVVSQNCLGSNVSLS LRLYIGKNSLPT in isoform 2. 1 PublicationAdd BLAST | 32 | |
Alternative sequenceiVSP_052506 | 615 – 917 | Missing in isoform 2. 1 PublicationAdd BLAST | 303 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY968204 mRNA Translation: AAY41069.1 AK047475 mRNA Translation: BAC33069.1 BC060733 mRNA Translation: AAH60733.1 BC067045 mRNA Translation: AAH67045.1 AF265663 mRNA Translation: AAL55724.1 |
CCDSi | CCDS19662.1 [Q2VPU4-1] CCDS39268.1 [Q2VPU4-2] |
RefSeqi | NP_598678.2, NM_133917.3 NP_808250.1, NM_177582.3 |
Genome annotation databases
GeneIDi | 208104 |
KEGGi | mmu:208104 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY968204 mRNA Translation: AAY41069.1 AK047475 mRNA Translation: BAC33069.1 BC060733 mRNA Translation: AAH60733.1 BC067045 mRNA Translation: AAH67045.1 AF265663 mRNA Translation: AAL55724.1 |
CCDSi | CCDS19662.1 [Q2VPU4-1] CCDS39268.1 [Q2VPU4-2] |
RefSeqi | NP_598678.2, NM_133917.3 NP_808250.1, NM_177582.3 |
3D structure databases
SMRi | Q2VPU4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 228948, 3 interactors |
STRINGi | 10090.ENSMUSP00000064943 |
PTM databases
iPTMneti | Q2VPU4 |
PhosphoSitePlusi | Q2VPU4 |
Proteomic databases
MaxQBi | Q2VPU4 |
PaxDbi | Q2VPU4 |
PRIDEi | Q2VPU4 |
ProteomicsDBi | 295682 [Q2VPU4-1] 295683 [Q2VPU4-2] |
Protocols and materials databases
DNASUi | 208104 |
Genome annotation databases
GeneIDi | 208104 |
KEGGi | mmu:208104 |
Organism-specific databases
CTDi | 22877 |
MGIi | MGI:2141183, Mlxip |
Phylogenomic databases
eggNOGi | KOG3582, Eukaryota |
InParanoidi | Q2VPU4 |
OrthoDBi | 388166at2759 |
PhylomeDBi | Q2VPU4 |
Miscellaneous databases
BioGRID-ORCSi | 208104, 1 hit in 65 CRISPR screens |
ChiTaRSi | Mlxip, mouse |
PROi | PR:Q2VPU4 |
RNActi | Q2VPU4, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR032648, MLXIP |
PANTHERi | PTHR15741:SF23, PTHR15741:SF23, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MLXIP_MOUSE | |
Accessioni | Q2VPU4Primary (citable) accession number: Q2VPU4 Secondary accession number(s): Q6NXJ4 Q8VI53 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 21, 2007 |
Last sequence update: | January 10, 2006 | |
Last modified: | February 23, 2022 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot