Mlxip

Functionⁱ

Binds DNA as a heterodimer with MLX and activates transcription. Binds to the canonical E box sequence 5'-CACGTG-3'. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.By similarity2 Publications

GO - Molecular functionⁱ

DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
DNA-binding transcription factor activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
protein dimerization activity Source: InterPro
RNA polymerase II regulatory region sequence-specific DNA binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding
Biological process	Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: MLX-interacting protein Alternative name(s): Transcriptional activator MondoA
Gene namesⁱ	Name:MlxipImported Synonyms:MirImported
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Unplaced

Organism-specific databases

MGIⁱ	MGI:2141183 Mlxip

Keywords - Cellular componentⁱ

Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedBy similarity
ChainⁱPRO_0000298764	2 – 917	MLX-interacting proteinAdd BLAST		916

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	2	N-acetylalanineBy similarity	1
Modified residueⁱ	9	PhosphoserineBy similarity	1
Modified residueⁱ	33	PhosphoserineBy similarity	1
Modified residueⁱ	39	PhosphoserineCombined sources	1
Modified residueⁱ	667	PhosphoserineBy similarity	1

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q2VPU4
PaxDbⁱ	Q2VPU4
PRIDEⁱ	Q2VPU4

PTM databases

iPTMnetⁱ	Q2VPU4
PhosphoSitePlusⁱ	Q2VPU4

Expressionⁱ

Developmental stageⁱ

Broadly expressed from 9.5 dpc to at least 15 dpc, with slightly elevated levels in the developing nervous system.1 Publication

Gene expression databases

CleanExⁱ	MM_MLXIP

CleanExⁱ

MM_MLXIP

Interactionⁱ

Subunit structureⁱ

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer with MLX.1 Publication

GO - Molecular functionⁱ

protein dimerization activity Source: InterPro

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	228948, 2 interactors
STRINGⁱ	10090.ENSMUSP00000064943

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q2VPU4
SMRⁱ	Q2VPU4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	717 – 767	bHLHPROSITE-ProRule annotationAdd BLAST		51

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	73 – 327	Required for cytoplasmic localizationBy similarityAdd BLAST	255
Regionⁱ	322 – 445	Transactivation domain1 PublicationAdd BLAST	124
Regionⁱ	767 – 788	Leucine-zipperAdd BLAST	22
Regionⁱ	830 – 879	Mediates heterotypic interactions between MLXIP and MLX and is required for cytoplasmic localizationBy similarityAdd BLAST	50

Phylogenomic databases

eggNOGⁱ	KOG3582 Eukaryota ENOG410XTA5 LUCA
HOGENOMⁱ	HOG000113606
HOVERGENⁱ	HBG062003
InParanoidⁱ	Q2VPU4
KEGG Orthology (KO) More...KOⁱ	K09113
PhylomeDBⁱ	Q2VPU4

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf IPR032648 MLXIP
PANTHERⁱ	PTHR15741:SF23 PTHR15741:SF23, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show all Align All

Isoform 11 Publication (identifier: Q2VPU4-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAADVFMCSP RRPRSRGRSV LLKPQVPEDD DDSDTDEPSP PPPSGVATSA 
        60         70         80         90        100
RAHASAAPLP PRAGPGREEP PRRQQIIHSG HFMVSSPHRE HPPKKGYDFD 
       110        120        130        140        150
TVNKQTCQTY SFGKTSSCHL SIDASLTKLF ECMTLAYSGK LVSPKWKNFK 
       160        170        180        190        200
GLKLQWRDKI RLNNAIWRAW YMQYLEKRRN PVCHFVTPLD GSVDVDEHRR 
       210        220        230        240        250
PEAITTEGKY WKSRIEIVIR EYHKWRTYFK KRLQQHKDED LSSLAQDDDM 
       260        270        280        290        300
LYWHKHGDGW KTPVPMEEDS LLDTDMLMSE FSDTLFSTLS SHQPVAWPNP 
       310        320        330        340        350
REIAHLGNAD MIQPGLIPLQ PNLDFMDTFE PFQDLFSSSR SIFGSMLPPP 
       360        370        380        390        400
SSLPAADPSS PPSQGNILPN TALPPASLPN SLITSSAAPS LDPTEGQGCE 
       410        420        430        440        450
RTSQTVDPFI QPADFGPSEP PLSVPQPFLP VFTMTLLSPG PAPAPVPTAL 
       460        470        480        490        500
PLVPSPAPTL NPPTPPAFLQ PQKFAGVSKS TPVITHTASA TLTHDASATT 
       510        520        530        540        550
FSQNQGLVIT AHHPTPSSSP CALALSPVPQ PPAVGPPQPH LTFIHPKPVS 
       560        570        580        590        600
LTGVRHKQPP KIVPAPKPEP VSLVLKNACI APAAFSGQPQ KVIMTSAPLK 
       610        620        630        640        650
REGILASTVS PSNVVIASAA ITRASGVTEF LSHSTSSQPS PVSRLFSPST 
       660        670        680        690        700
VQDSLVKGEQ VSLHGGSPQV PATGSSRDCP NSGQASPCPS EQSPSPQSPQ 
       710        720        730        740        750
NNCSGKSTDP KNVAALKNRQ KHISAEQKRR FNIRMGFNTL NSLISNNSKQ 
       760        770        780        790        800
TSHAITLQKT MEYITKLQQE RMQMQEEARR LREEIEELNT TIISCQQLLP 
       810        820        830        840        850
ATGVPVNCRQ LDHMRDMFDE YVKSRTLQNW KFWIFSMIIK PLFESFKGMV 
       860        870        880        890        900
STSSLEEFHR TALSWLDQHC SLPVLRPMVL STLRQLSTTT SILTDPSQLP 
       910 
EQASEAVTRM GKRSGES

Length:917

Mass (Da):100,804

Last modified:January 10, 2006 - v1

Checksum:ⁱ2944C041E78F89FC

GO

Isoform 21 Publication (identifier: Q2VPU4-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
583-614: AAFSGQPQKVIMTSAPLKREGILASTVSPSNV → GELVGGVVSQNCLGSNVSLSLRLYIGKNSLPT
615-917: Missing.

« Hide

        10         20         30         40         50
MAADVFMCSP RRPRSRGRSV LLKPQVPEDD DDSDTDEPSP PPPSGVATSA 
        60         70         80         90        100
RAHASAAPLP PRAGPGREEP PRRQQIIHSG HFMVSSPHRE HPPKKGYDFD 
       110        120        130        140        150
TVNKQTCQTY SFGKTSSCHL SIDASLTKLF ECMTLAYSGK LVSPKWKNFK 
       160        170        180        190        200
GLKLQWRDKI RLNNAIWRAW YMQYLEKRRN PVCHFVTPLD GSVDVDEHRR 
       210        220        230        240        250
PEAITTEGKY WKSRIEIVIR EYHKWRTYFK KRLQQHKDED LSSLAQDDDM 
       260        270        280        290        300
LYWHKHGDGW KTPVPMEEDS LLDTDMLMSE FSDTLFSTLS SHQPVAWPNP 
       310        320        330        340        350
REIAHLGNAD MIQPGLIPLQ PNLDFMDTFE PFQDLFSSSR SIFGSMLPPP 
       360        370        380        390        400
SSLPAADPSS PPSQGNILPN TALPPASLPN SLITSSAAPS LDPTEGQGCE 
       410        420        430        440        450
RTSQTVDPFI QPADFGPSEP PLSVPQPFLP VFTMTLLSPG PAPAPVPTAL 
       460        470        480        490        500
PLVPSPAPTL NPPTPPAFLQ PQKFAGVSKS TPVITHTASA TLTHDASATT 
       510        520        530        540        550
FSQNQGLVIT AHHPTPSSSP CALALSPVPQ PPAVGPPQPH LTFIHPKPVS 
       560        570        580        590        600
LTGVRHKQPP KIVPAPKPEP VSLVLKNACI APGELVGGVV SQNCLGSNVS 
       610 
LSLRLYIGKN SLPT

Note: No experimental confirmation available.Curated

Show »

Length:614

Mass (Da):67,085

Checksum:ⁱB72C6C2905A8EA3E

GO

Computationally mapped potential isoform sequencesⁱ

There are 3 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

G5E8D8	G5E8D8_MOUSE	MLX interacting protein, isoform CR... MLX interacting protein, isoform CRA_a (MLX-interacting protein)	Mlxip mCG_142189	917	Annotation score:
F6UPM1	F6UPM1_MOUSE	MLX-interacting protein MLX-interacting protein	Mlxip	241	Annotation score:
B2RQ56	B2RQ56_MOUSE	MLX-interacting protein MLX-interacting protein	Mlxip	614	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	179	R → K in BAC33069 (PubMed:16141072).Curated		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_052505	583 – 614	AAFSG…SPSNV → GELVGGVVSQNCLGSNVSLS LRLYIGKNSLPT in isoform 2. 1 PublicationAdd BLAST		32
Alternative sequenceⁱVSP_052506	615 – 917	Missing in isoform 2. 1 PublicationAdd BLAST		303

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY968204 mRNA Translation: AAY41069.1 AK047475 mRNA Translation: BAC33069.1 BC060733 mRNA Translation: AAH60733.1 BC067045 mRNA Translation: AAH67045.1 AF265663 mRNA Translation: AAL55724.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS19662.1 [Q2VPU4-1] CCDS39268.1 [Q2VPU4-2]
NCBI Reference Sequences More...RefSeqⁱ	NP_598678.2, NM_133917.3 NP_808250.1, NM_177582.3
UniGeneⁱ	Mm.83277

Genome annotation databases

GeneIDⁱ	208104
KEGGⁱ	mmu:208104

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q2VPU4	MLX interacting protein, isoform CRA_a		MOUSE		917	UniRef90_Q2VPU4
MLX-interacting protein		RAT		834
MLX-interacting protein		CRIGR		830
UPI0004ED0A5B		NANGA		929
UPI000A307756		MUSPA		917
+8
Q2VPU4-2	MLX-interacting protein		MOUSE		614	UniRef90_Q2VPU4-2

Protein	Similar proteins		Species	Score	Length	Source
Q2VPU4	MLX-interacting protein		HUMAN		919	UniRef50_Q9HAP2
MLX interacting protein		CALJA		979
Uncharacterized protein		MACMU		931
MLX-interacting protein		TARSY		925
Uncharacterized protein		PROCO		925
+237
Q2VPU4-2	MLX-interacting protein		MOUSE		614	UniRef50_Q2VPU4-2
MLX interacting protein		SARHA		706

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY968204 mRNA Translation: AAY41069.1 AK047475 mRNA Translation: BAC33069.1 BC060733 mRNA Translation: AAH60733.1 BC067045 mRNA Translation: AAH67045.1 AF265663 mRNA Translation: AAL55724.1
CCDSⁱ	CCDS19662.1 [Q2VPU4-1] CCDS39268.1 [Q2VPU4-2]
RefSeqⁱ	NP_598678.2, NM_133917.3 NP_808250.1, NM_177582.3
UniGeneⁱ	Mm.83277

3D structure databases

ProteinModelPortalⁱ	Q2VPU4
SMRⁱ	Q2VPU4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	228948, 2 interactors
STRINGⁱ	10090.ENSMUSP00000064943

PTM databases

iPTMnetⁱ	Q2VPU4
PhosphoSitePlusⁱ	Q2VPU4

Proteomic databases

MaxQBⁱ	Q2VPU4
PaxDbⁱ	Q2VPU4
PRIDEⁱ	Q2VPU4

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	208104
KEGGⁱ	mmu:208104

Organism-specific databases

CTDⁱ	22877
MGIⁱ	MGI:2141183 Mlxip

Phylogenomic databases

eggNOGⁱ	KOG3582 Eukaryota ENOG410XTA5 LUCA
HOGENOMⁱ	HOG000113606
HOVERGENⁱ	HBG062003
InParanoidⁱ	Q2VPU4
KOⁱ	K09113
PhylomeDBⁱ	Q2VPU4

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q2VPU4
SOURCEⁱ	Search...

Gene expression databases

CleanExⁱ	MM_MLXIP

CleanExⁱ

MM_MLXIP

Family and domain databases

CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf IPR032648 MLXIP
PANTHERⁱ	PTHR15741:SF23 PTHR15741:SF23, 1 hit
Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MLXIP_MOUSE
Accessionⁱ	Q2VPU4Primary (citable) accession number: Q2VPU4 Secondary accession number(s): Q6NXJ4 , Q6P9J8, Q8C8C6, Q8VI53
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
	Last sequence update:	January 10, 2006
	Last modified:	November 7, 2018
	This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q2VPU4 (MLXIP_MOUSE)

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MLX-interacting protein

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Mitochondrion

Nucleus

Other locations

Mitochondrion

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Phylogenomic databases

Family and domain databases

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ