UniProtKB - Q2VF19 (DCL1_CRYPA)
Protein
Dicer-like protein 1
Gene
DCL-1
Organism
Cryphonectria parasitica (Chestnut blight fungus) (Endothia parasitica)
Status
Functioni
Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons (By similarity).By similarity1 Publication
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 1288 | Magnesium or manganeseBy similarity | 1 | |
Sitei | 1393 | Important for activityBy similarity | 1 | |
Metal bindingi | 1397 | Magnesium or manganeseBy similarity | 1 | |
Metal bindingi | 1400 | Magnesium or manganeseBy similarity | 1 | |
Metal bindingi | 1457 | ZincBy similarity | 1 | |
Metal bindingi | 1489 | Zinc; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 1530 | ZincBy similarity | 1 | |
Metal bindingi | 1532 | ZincBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 119 – 126 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA binding Source: InterPro
- helicase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- ribonuclease III activity Source: InterPro
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- defense response to virus Source: UniProtKB-KW
- regulation of defense response to virus Source: UniProtKB-KW
- RNA processing Source: InterPro
Keywordsi
Molecular function | Antiviral protein, Helicase, Hydrolase, RNA-binding |
Biological process | Antiviral defense |
Ligand | ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, Zinc |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:DCL-1 |
Organismi | Cryphonectria parasitica (Chestnut blight fungus) (Endothia parasitica) |
Taxonomic identifieri | 5116 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Diaporthales › Cryphonectriaceae › Cryphonectria-Endothia species complex › Cryphonectria |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000306780 | 1 – 1548 | Dicer-like protein 1Add BLAST | 1548 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 106 – 289 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 184 | |
Domaini | 428 – 589 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 162 | |
Domaini | 624 – 718 | Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd BLAST | 95 | |
Domaini | 1051 – 1197 | RNase III 1PROSITE-ProRule annotationAdd BLAST | 147 | |
Domaini | 1248 – 1411 | RNase III 2PROSITE-ProRule annotationAdd BLAST | 164 | |
Domaini | 1445 – 1518 | DRBMAdd BLAST | 74 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 232 – 235 | DEAH box | 4 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
OMAi | HHPTKIL |
Family and domain databases
CDDi | cd00593, RIBOc, 2 hits |
Gene3Di | 1.10.1520.10, 2 hits 3.30.160.380, 1 hit |
InterProi | View protein in InterPro IPR038248, Dicer_dimer_sf IPR005034, Dicer_dimerisation_dom IPR006935, Helicase/UvrB_N IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000999, RNase_III_dom IPR036389, RNase_III_sf |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF04851, ResIII, 1 hit PF00636, Ribonuclease_3, 2 hits |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit SM00535, RIBOc, 2 hits |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF69065, SSF69065, 2 hits |
PROSITEi | View protein in PROSITE PS51327, DICER_DSRBF, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS00517, RNASE_3_1, 1 hit PS50142, RNASE_3_2, 2 hits |
i Sequence
Sequence statusi: Complete.
Q2VF19-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGDPAAHEMA DLERGFSSED DAEYRSGDDE ASKFVENEPS KRGKISQKKK
60 70 80 90 100
IEQTNFAKWM DTNQKSLTRK AVKQSVNQDN SLAYMIRSWE GGEKIITSPR
110 120 130 140 150
DYQMELFERA KQQNTIAVLD TGSGKTLIAA LLLDHTVNQE LEDRAKGLPR
160 170 180 190 200
RIAFFLVEKV ALAFQQHAVL ECNLAHSVAV FSGESIKNTW TKGFWETQLA
210 220 230 240 250
DHEVIVCTAE ILNQCLQYAY IRIDQINLLV FDEAHHTKKN HPYARIIKDY
260 270 280 290 300
YASGKDRGLR LPRIFGMTAS PVDALIDVRQ AAIELEGLLH SRIATTADPD
310 320 330 340 350
ALRRAVGRPK KEIIYKYNPL VKPIQTMLTF KLRPLIANNK QFSKAFAFSE
360 370 380 390 400
AAARELGTWF VDRMWQLFLE DEELLKLEAK TERSLSKDMA APEVVEKHRN
410 420 430 440 450
AVRSARELIR SHEFPKPEPG LLSSKLKTLS KLLEEYFTDS SIRCIVFVER
460 470 480 490 500
RWTAKLLTDF FESHAAEIPG LKVGSLMGAN AEGGSSQTSF REQIRTILSF
510 520 530 540 550
KKGNTNCIFA TSVAEEGLDI PDCNLIIRFD ICKTMIQYIQ SRGRARQADS
560 570 580 590 600
TYIHLIEGGN GDHRRIMHQN AENEKLLRRF CNTQPEDRLL KGSDYDMDFF
610 620 630 640 650
LRQERNQRQY TIKSTGARLT YKNSLPILQA FLNTLRNQDD YAEGMDLVAD
660 670 680 690 700
YSILSVQGGF ICEVMMPPLS PVTSAIGKVY STKQVAKCSA AFELCFQLIQ
710 720 730 740 750
KKFLDDHLRS KFVEKRHVMA NARLAVSSKN KAKYDMRLKP QIWAELGVPE
760 770 780 790 800
KLYATVLILS KPSALERPSR PLFILTRTPL PQLKPFLLFL GPVEQEMTSD
810 820 830 840 850
LVCQVLNCPI TPTEEDLQLL TKFTLKIFVD IFNKKYAANA QALPYFFAPT
860 870 880 890 900
NKDHVFLFSN LQDPRNAVDW PLLRHVADRD AEAYTGDEPE EFFQDKYIVD
910 920 930 940 950
PHDGARRFWL QGIRKDLTCT SPVPADVEHQ PTHRQWKRRE VPHDILHWSL
960 970 980 990 1000
TAWKATREAH ENKWKENQPV VVGKYATLRR NFLADINETS KNPFCYFVLE
1010 1020 1030 1040 1050
PMRISPLPVD VVAMAYLLPS IIHRIEQNLI ALDACRLLQL DIHPDLALEA
1060 1070 1080 1090 1100
LTKDSDNQGE DERMDSIQAF EPVNFQPGMG ANYERLELLG DSFLKMATTI
1110 1120 1130 1140 1150
AVFTLIPNKD EFDYHCERMV MICNQNLFGV AKSDDLKLHE YIRSKSFERG
1160 1170 1180 1190 1200
TWYPVLKLEF GKTHLKTLKQ MDEHRLADKS IADVCEALIG AAYMTTRKHD
1210 1220 1230 1240 1250
DYDLAVRAVT RLVNHKQHPM TKWDDYHAAY VMPGWQTMPA NAAELDMAQK
1260 1270 1280 1290 1300
IHEATGYQFK HPRVLRSAFR HPSRPYVFDK VPHYQRLEFL GDALFDMACV
1310 1320 1330 1340 1350
DYLFHIAPDE GPQWLTEHKM AMVSNQFLGC LAVSLGFHKF ILHHHASIGS
1360 1370 1380 1390 1400
QIHEYVTEIT EARRAAEDAA EAAGKPRSAY SRDYWVEAPQ PPKCIPDVLE
1410 1420 1430 1440 1450
AYVGAIFVDS KYDYSVVQQF FHAHVLPFFA SMRMYDTFAN KHPVTFFTQY
1460 1470 1480 1490 1500
VFETFGCHAY GLHAEEMPVK DDTGLVTGKT QVVAGILLHG QVVEGAVRDS
1510 1520 1530 1540
GRYAKIAAAR KALDKLRSMT RQEFLDAYKC DCKPGEAAED ISESATAI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ186989 Genomic DNA Translation: ABB00356.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ186989 Genomic DNA Translation: ABB00356.1 |
3D structure databases
SMRi | Q2VF19 |
ModBasei | Search... |
Phylogenomic databases
OMAi | HHPTKIL |
Family and domain databases
CDDi | cd00593, RIBOc, 2 hits |
Gene3Di | 1.10.1520.10, 2 hits 3.30.160.380, 1 hit |
InterProi | View protein in InterPro IPR038248, Dicer_dimer_sf IPR005034, Dicer_dimerisation_dom IPR006935, Helicase/UvrB_N IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000999, RNase_III_dom IPR036389, RNase_III_sf |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF04851, ResIII, 1 hit PF00636, Ribonuclease_3, 2 hits |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit SM00535, RIBOc, 2 hits |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF69065, SSF69065, 2 hits |
PROSITEi | View protein in PROSITE PS51327, DICER_DSRBF, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS00517, RNASE_3_1, 1 hit PS50142, RNASE_3_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DCL1_CRYPA | |
Accessioni | Q2VF19Primary (citable) accession number: Q2VF19 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 2, 2007 |
Last sequence update: | January 10, 2006 | |
Last modified: | October 7, 2020 | |
This is version 67 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |