UniProtKB - Q2U6C4 (DCL1_ASPOR)
Protein
Dicer-like protein 1
Gene
dcl1
Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Functioni
Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons (By similarity).By similarity
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 1281 | Magnesium or manganeseBy similarity | 1 | |
Sitei | 1374 | Important for activityBy similarity | 1 | |
Metal bindingi | 1378 | Magnesium or manganeseBy similarity | 1 | |
Metal bindingi | 1381 | Magnesium or manganeseBy similarity | 1 | |
Metal bindingi | 1438 | ZincBy similarity | 1 | |
Metal bindingi | 1465 | Zinc; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 1506 | ZincBy similarity | 1 | |
Metal bindingi | 1508 | ZincBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 136 – 143 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA binding Source: InterPro
- helicase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- ribonuclease III activity Source: InterPro
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- defense response to virus Source: UniProtKB-KW
- regulation of defense response to virus Source: UniProtKB-KW
- RNA processing Source: InterPro
Keywordsi
Molecular function | Antiviral protein, Helicase, Hydrolase, RNA-binding |
Biological process | Antiviral defense |
Ligand | ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, Zinc |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:dcl1 ORF Names:AO090120000297 |
Organismi | Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
Taxonomic identifieri | 510516 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › Aspergillus subgen. Circumdati › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000306776 | 1 – 1523 | Dicer-like protein 1Add BLAST | 1523 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 123 – 304 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 182 | |
Domaini | 444 – 617 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 174 | |
Domaini | 640 – 730 | Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd BLAST | 91 | |
Domaini | 1031 – 1190 | RNase III 1PROSITE-ProRule annotationAdd BLAST | 160 | |
Domaini | 1241 – 1392 | RNase III 2PROSITE-ProRule annotationAdd BLAST | 152 | |
Domaini | 1426 – 1494 | DRBMAdd BLAST | 69 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 249 – 252 | DEAH box | 4 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
OMAi | FNMYVQT |
Family and domain databases
CDDi | cd00593, RIBOc, 2 hits |
Gene3Di | 1.10.1520.10, 2 hits 3.30.160.380, 1 hit |
InterProi | View protein in InterPro IPR038248, Dicer_dimer_sf IPR005034, Dicer_dimerisation_dom IPR006935, Helicase/UvrB_N IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000999, RNase_III_dom IPR036389, RNase_III_sf |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF04851, ResIII, 1 hit PF00636, Ribonuclease_3, 2 hits |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit SM00535, RIBOc, 2 hits |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF69065, SSF69065, 2 hits |
PROSITEi | View protein in PROSITE PS51327, DICER_DSRBF, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS00517, RNASE_3_1, 1 hit PS50142, RNASE_3_2, 2 hits |
i Sequence
Sequence statusi: Complete.
Q2U6C4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPPLEVKPLA GYVRSQSLRI PSSLNLSGER TISTTEPTEG NDSSSEESGD
60 70 80 90 100
NEQISTQRLI SQNKRLQSAK FEALLSERAD TLTGNSGRPT LDLPDAELST
110 120 130 140 150
ASLVAKQDAG TGMLDPREYQ VELFERAKSQ NTIAVLDTGS GKTLIAVLLL
160 170 180 190 200
KHIIQNELID RANGKPPRIS FFLVDSVTLA FQQAAVLRNN LDQNVAQFFG
210 220 230 240 250
AMGTDLWSKQ TWDHQFENNM VIVCTAEILN QCLLNSYIRM DQINLLIFDE
260 270 280 290 300
AHHTKKDHPY ARIIRESYLK ADPTKRPRIF GMTASPIDTK GDIIESATKL
310 320 330 340 350
EVLLDSKIAT TSKPNLLREV VRRPIEESWE YDKLDPPFAT KLYQILQARF
360 370 380 390 400
GDISSLQPVF RFTLQASSEL GPCCADRAWA YALADDVLPK LEGNVRKLAQ
410 420 430 440 450
SISSPIPQCA LKEISRIQEA SDIVKNHSFN SPNVPGELSP KVQLLRQKLI
460 470 480 490 500
KYFEHPTKTK CIVFTQKRYT AKMLFDLFST LEIPYLRPGV LIGVRSGDIV
510 520 530 540 550
GMNVSFRQQF LALVKFRSGE INCLFATSVA EEGLDIPDCN LVVRFDLYNT
560 570 580 590 600
LIQYVQSRGR ARHSSSTYAS MIERYNADHA ARLVEVREAE KLMQSFCETL
610 620 630 640 650
PEDRILHGID SEIDSILQGE EEKRTFIIRA TGAKLTYHSA LAILARYASS
660 670 680 690 700
LQYEKETSAQ ATYVVLPQNN SFVCEVILPE KSPVRGLTGV PASKKSAAKQ
710 720 730 740 750
SAAFDTCVLL RKHKLLDDHF NSVYHRRLPA MRNARLAITS SRTNQYDMLS
760 770 780 790 800
KPSLWGKQQG TLPEKLFATV ISFIPSEPLR RRHRSIILLT RERLPDFPSF
810 820 830 840 850
TIFLDDDIET IVVTESVEEA LHISSQELEY LSTFTFRIFH DVFHKTYAEE
860 870 880 890 900
PEKLPYWVAP AETKKSKNVS DSKSLTDWEL LHLVHENEEI PSTLHPSSEA
910 920 930 940 950
LINRFVFDPW DGRYRYFTMA IDNTLHPSDP PPSFLPRRKF MESIMSYTLS
960 970 980 990 1000
GSKNARAGFL SRCNWQQPVL EVELVRLRRN LLDKMTDTEK DVETRCFVCI
1010 1020 1030 1040 1050
EPLRISAIPE EIAASCLAFP AIINRLDAYL IALEGCKTLD LSVKPEYALE
1060 1070 1080 1090 1100
AFTKDSDNTE EHRVQQIHVQ RGMGKNYERL EFLGDCFLKM ATSISLFVQN
1110 1120 1130 1140 1150
PDDDEFDFHV NRMCLICNKN LFNTALKKEL YQYTRSRGFS RHTWYPDGLT
1160 1170 1180 1190 1200
LLHGRDHRKK ISAESKHALR EKTVADVCEA LIGASLLSGG LHNQFDMAVK
1210 1220 1230 1240 1250
AVTAVVDSPN HKALCWADYT SSYMLPKYQT QSPDGYELDL GRKVEEKLGY
1260 1270 1280 1290 1300
RFKYPRLLHS AFTHPSYPST WAKVPCYQRL EFLGDSLLDM VCVDDLFYRY
1310 1320 1330 1340 1350
PDKDPQWLTE HKMAMVSNKF LGALAVKLGL HTHLRHFSNP LQSQITHYAE
1360 1370 1380 1390 1400
EIQAAENESQ GAVDYWLVTK DPPKCLPDMV EAYLGAAFVD SDFQFRVVED
1410 1420 1430 1440 1450
FFQRHVKSYF HDMTIYDTFA NKHPTTFLQN RLTNEYGCTN YCLKAGEIPV
1460 1470 1480 1490 1500
VDGGTVSVLA AVIVHEVVIA EGTASSGRYA KVKASEKALS VLENMGPSEF
1510 1520
REKYHCDCRT ANGSQPMDIG TAI
Sequence cautioni
The sequence BAE62891 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AP007166 Genomic DNA Translation: BAE62891.1 Sequence problems. |
RefSeqi | XP_001824024.2, XM_001823972.2 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AP007166 Genomic DNA Translation: BAE62891.1 Sequence problems. |
RefSeqi | XP_001824024.2, XM_001823972.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Phylogenomic databases
OMAi | FNMYVQT |
Family and domain databases
CDDi | cd00593, RIBOc, 2 hits |
Gene3Di | 1.10.1520.10, 2 hits 3.30.160.380, 1 hit |
InterProi | View protein in InterPro IPR038248, Dicer_dimer_sf IPR005034, Dicer_dimerisation_dom IPR006935, Helicase/UvrB_N IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000999, RNase_III_dom IPR036389, RNase_III_sf |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF04851, ResIII, 1 hit PF00636, Ribonuclease_3, 2 hits |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit SM00535, RIBOc, 2 hits |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF69065, SSF69065, 2 hits |
PROSITEi | View protein in PROSITE PS51327, DICER_DSRBF, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS00517, RNASE_3_1, 1 hit PS50142, RNASE_3_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DCL1_ASPOR | |
Accessioni | Q2U6C4Primary (citable) accession number: Q2U6C4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 2, 2007 |
Last sequence update: | October 2, 2007 | |
Last modified: | April 7, 2021 | |
This is version 93 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families