UniProtKB - Q2TX99 (ITPA_ASPOR)
Protein
Inosine triphosphate pyrophosphatase
Gene
AO090010000228
Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Functioni
Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.UniRule annotation
Catalytic activityi
- a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-phosphate + diphosphate + H+UniRule annotationEC:3.6.1.9UniRule annotation
- a 2'-deoxyribonucleoside 5'-triphosphate + H2O = a 2'-deoxyribonucleoside 5'-phosphate + diphosphate + H+UniRule annotationEC:3.6.1.9UniRule annotation
Cofactori
Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 divalent metal cation per subunit; can use either Mg2+ or Mn2+.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 40 | Magnesium or manganeseUniRule annotation | 1 | |
Binding sitei | 52 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 68 | Magnesium or manganeseUniRule annotation | 1 | |
Binding sitei | 167 | SubstrateUniRule annotation | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- NADH pyrophosphatase activity Source: UniProtKB-EC
- nucleoside-triphosphate diphosphatase activity Source: UniProtKB-UniRule
- nucleotide binding Source: UniProtKB-KW
GO - Biological processi
- deoxyribonucleoside triphosphate catabolic process Source: UniProtKB-UniRule
- nucleotide metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase |
Biological process | Nucleotide metabolism |
Ligand | Magnesium, Manganese, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Inosine triphosphate pyrophosphataseUniRule annotation (EC:3.6.1.9UniRule annotation)Short name: ITPaseUniRule annotation Short name: Inosine triphosphataseUniRule annotation Alternative name(s): Non-canonical purine NTP pyrophosphataseUniRule annotation Non-standard purine NTP pyrophosphataseUniRule annotation Nucleoside-triphosphate diphosphataseUniRule annotation Nucleoside-triphosphate pyrophosphataseUniRule annotation Short name: NTPaseUniRule annotation |
Gene namesi | ORF Names:AO090010000228 |
Organismi | Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
Taxonomic identifieri | 510516 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000413128 | 1 – 191 | Inosine triphosphate pyrophosphataseAdd BLAST | 191 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 12 – 17 | Substrate bindingUniRule annotation | 6 | |
Regioni | 68 – 69 | Substrate bindingUniRule annotation | 2 | |
Regioni | 144 – 147 | Substrate bindingUniRule annotation | 4 | |
Regioni | 172 – 173 | Substrate bindingUniRule annotation | 2 |
Sequence similaritiesi
Belongs to the HAM1 NTPase family.UniRule annotation
Phylogenomic databases
HOGENOMi | HOG000293320 |
OMAi | GYCDGRK |
Family and domain databases
CDDi | cd00515 HAM1, 1 hit |
Gene3Di | 3.90.950.10, 1 hit |
HAMAPi | MF_03148 HAM1_NTPase, 1 hit |
InterProi | View protein in InterPro IPR002637 Ham1p-like IPR027502 ITPase IPR029001 ITPase-like_fam |
PANTHERi | PTHR11067 PTHR11067, 1 hit |
Pfami | View protein in Pfam PF01725 Ham1p_like, 1 hit |
SUPFAMi | SSF52972 SSF52972, 1 hit |
TIGRFAMsi | TIGR00042 TIGR00042, 1 hit |
i Sequence
Sequence statusi: Complete.
Q2TX99-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTDSDNPLIL VTGNKNKVLE VKAILGPTAT LEVLDINLPE IQGSVEEITR
60 70 80 90 100
EKCRAAAETI GGPVLVEDSA LEMRALGGLP GAYVKAFVET IGNEGLNRIL
110 120 130 140 150
SAFDDKSAEA VCTFGYSQGP GHEPLLFQGR LQGRIVPARG VSSFGWEPIF
160 170 180 190
EVEGEGVTLA EMEVGKKNGL SHRFKALVKF REWFLGARRP V
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AP007175 Genomic DNA Translation: BAE66124.1 |
Genome annotation databases
EnsemblFungii | BAE66124; BAE66124; AO090010000228 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AP007175 Genomic DNA Translation: BAE66124.1 |
3D structure databases
SMRi | Q2TX99 |
ModBasei | Search... |
Genome annotation databases
EnsemblFungii | BAE66124; BAE66124; AO090010000228 |
Phylogenomic databases
HOGENOMi | HOG000293320 |
OMAi | GYCDGRK |
Family and domain databases
CDDi | cd00515 HAM1, 1 hit |
Gene3Di | 3.90.950.10, 1 hit |
HAMAPi | MF_03148 HAM1_NTPase, 1 hit |
InterProi | View protein in InterPro IPR002637 Ham1p-like IPR027502 ITPase IPR029001 ITPase-like_fam |
PANTHERi | PTHR11067 PTHR11067, 1 hit |
Pfami | View protein in Pfam PF01725 Ham1p_like, 1 hit |
SUPFAMi | SSF52972 SSF52972, 1 hit |
TIGRFAMsi | TIGR00042 TIGR00042, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ITPA_ASPOR | |
Accessioni | Q2TX99Primary (citable) accession number: Q2TX99 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 19, 2011 |
Last sequence update: | January 24, 2006 | |
Last modified: | May 8, 2019 | |
This is version 74 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families