UniProtKB - Q2T9Y6 (GSH0_BOVIN)
Protein
Glutamate--cysteine ligase regulatory subunit
Gene
GCLM
Organism
Bos taurus (Bovine)
Status
Functioni
: glutathione biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Glutamate--cysteine ligase (GCLC), Glutamate--cysteine ligase (GCLC), Glutamate--cysteine ligase (GCLC), Glutamate--cysteine ligase regulatory subunit (GCLM), Glutamate--cysteine ligase (GCLC), Glutamate--cysteine ligase (GCLC)
- Glutathione synthetase (GSS), Glutathione synthetase (GSS)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.
GO - Molecular functioni
- enzyme regulator activity Source: GO_Central
- glutamate-cysteine ligase activity Source: Ensembl
- glutamate-cysteine ligase catalytic subunit binding Source: UniProtKB
GO - Biological processi
- apoptotic mitochondrial changes Source: Ensembl
- cellular response to leukemia inhibitory factor Source: Ensembl
- cysteine metabolic process Source: Ensembl
- glutamate metabolic process Source: UniProtKB
- glutathione biosynthetic process Source: UniProtKB
- negative regulation of extrinsic apoptotic signaling pathway Source: Ensembl
- positive regulation of glutamate-cysteine ligase activity Source: GO_Central
- regulation of blood vessel diameter Source: UniProtKB
- regulation of mitochondrial depolarization Source: Ensembl
- response to drug Source: UniProtKB
- response to oxidative stress Source: UniProtKB
Keywordsi
Biological process | Glutathione biosynthesis |
Enzyme and pathway databases
Reactomei | R-BTA-174403, Glutathione synthesis and recycling |
UniPathwayi | UPA00142;UER00209 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamate--cysteine ligase regulatory subunitAlternative name(s): GCS light chain Gamma-ECS regulatory subunit Gamma-glutamylcysteine synthetase regulatory subunit Glutamate--cysteine ligase modifier subunit |
Gene namesi | Name:GCLM |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:29290, GCLM |
Subcellular locationi
Other locations
- glutamate-cysteine ligase complex Source: UniProtKB
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000253731 | 1 – 274 | Glutamate--cysteine ligase regulatory subunitAdd BLAST | 274 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 59 | PhosphoserineBy similarity | 1 | |
Modified residuei | 263 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | Q2T9Y6 |
PRIDEi | Q2T9Y6 |
PTM databases
SwissPalmi | Q2T9Y6 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000007842, Expressed in liver and 18 other tissues |
Interactioni
Subunit structurei
Heterodimer of a catalytic heavy chain and a regulatory light chain.
By similarityGO - Molecular functioni
- glutamate-cysteine ligase catalytic subunit binding Source: UniProtKB
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000026855 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3023, Eukaryota |
GeneTreei | ENSGT00510000047658 |
HOGENOMi | CLU_055657_1_0_1 |
InParanoidi | Q2T9Y6 |
OMAi | PYWEELQ |
OrthoDBi | 1516283at2759 |
TreeFami | TF105986 |
Family and domain databases
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR032963, Gclm IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
PANTHERi | PTHR13295, PTHR13295, 1 hit |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
SUPFAMi | SSF51430, SSF51430, 1 hit |
i Sequence
Sequence statusi: Complete.
Q2T9Y6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGTDSRAAGA LLARASTLHL QTGNLLNWGR LRKKCPSTHS EELRDCIQKT
60 70 80 90 100
LNEWSSQISP DLIREFPDVL ECTVSHAVEK INPDEREEMK VSAKLFIVGS
110 120 130 140 150
NSSSSTRNAV DMACSVLGVA QLDSVIIASP PVEDGVNLSL EHLQPYWEEL
160 170 180 190 200
QNLVQSKKIV AIGTSDLDKT QLEQLYQWAQ VKPNSNQVNL ASCCVMPPDL
210 220 230 240 250
TAFAKQFDIQ LLTHNDPKEL LSEASFQEAL QESIPDIRAH EWVPLWLLRY
260 270
SVIVKSRGII KSKGYILQAK RKGS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC111206 mRNA Translation: AAI11207.1 |
RefSeqi | NP_001033232.1, NM_001038143.1 |
Genome annotation databases
Ensembli | ENSBTAT00000026855; ENSBTAP00000026855; ENSBTAG00000007842 |
GeneIDi | 525659 |
KEGGi | bta:525659 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC111206 mRNA Translation: AAI11207.1 |
RefSeqi | NP_001033232.1, NM_001038143.1 |
3D structure databases
SMRi | Q2T9Y6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000026855 |
PTM databases
SwissPalmi | Q2T9Y6 |
Proteomic databases
PaxDbi | Q2T9Y6 |
PRIDEi | Q2T9Y6 |
Genome annotation databases
Ensembli | ENSBTAT00000026855; ENSBTAP00000026855; ENSBTAG00000007842 |
GeneIDi | 525659 |
KEGGi | bta:525659 |
Organism-specific databases
CTDi | 2730 |
VGNCi | VGNC:29290, GCLM |
Phylogenomic databases
eggNOGi | KOG3023, Eukaryota |
GeneTreei | ENSGT00510000047658 |
HOGENOMi | CLU_055657_1_0_1 |
InParanoidi | Q2T9Y6 |
OMAi | PYWEELQ |
OrthoDBi | 1516283at2759 |
TreeFami | TF105986 |
Enzyme and pathway databases
UniPathwayi | UPA00142;UER00209 |
Reactomei | R-BTA-174403, Glutathione synthesis and recycling |
Gene expression databases
Bgeei | ENSBTAG00000007842, Expressed in liver and 18 other tissues |
Family and domain databases
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR032963, Gclm IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
PANTHERi | PTHR13295, PTHR13295, 1 hit |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
SUPFAMi | SSF51430, SSF51430, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GSH0_BOVIN | |
Accessioni | Q2T9Y6Primary (citable) accession number: Q2T9Y6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 17, 2006 |
Last sequence update: | January 24, 2006 | |
Last modified: | December 2, 2020 | |
This is version 95 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families