Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

S-adenosylmethionine synthase

Gene

metK

Organism
Moorella thermoacetica (strain ATCC 39073 / JCM 9320)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.UniRule annotation

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase (metK)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15ATPUniRule annotation1
Metal bindingi17MagnesiumUniRule annotation1
Metal bindingi43PotassiumUniRule annotation1
Binding sitei99MethionineUniRule annotation1
Binding sitei249ATP; shared with neighboring subunitUniRule annotation1
Binding sitei249Methionine; shared with neighboring subunitUniRule annotation1
Binding sitei272ATP; via amide nitrogen; shared with neighboring subunitUniRule annotation1
Binding sitei276ATP; shared with neighboring subunitUniRule annotation1
Binding sitei280MethionineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi174 – 176ATPUniRule annotation3
Nucleotide bindingi240 – 241ATPUniRule annotation2
Nucleotide bindingi255 – 256ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processOne-carbon metabolism
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciMTHE264732:G1G5J-929-MONOMER
UniPathwayi
UPA00315;UER00080

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthaseUniRule annotation (EC:2.5.1.6UniRule annotation)
Short name:
AdoMet synthaseUniRule annotation
Alternative name(s):
MATUniRule annotation
Methionine adenosyltransferaseUniRule annotation
Gene namesi
Name:metKUniRule annotation
Ordered Locus Names:Moth_0894
OrganismiMoorella thermoacetica (strain ATCC 39073 / JCM 9320)
Taxonomic identifieri264732 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeMoorella groupMoorella
Proteomesi
  • UP000007053 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002410051 – 395S-adenosylmethionine synthaseAdd BLAST395

Proteomic databases

PRIDEiQ2RK28

Interactioni

Subunit structurei

Homotetramer; dimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi264732.Moth_0894

Structurei

3D structure databases

SMRiQ2RK28
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni99 – 109Flexible loopUniRule annotationAdd BLAST11

Sequence similaritiesi

Belongs to the AdoMet synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPH Bacteria
COG0192 LUCA
HOGENOMiHOG000245710
KOiK00789
OMAiPGHFLFT
OrthoDBiPOG091H00IO

Family and domain databases

HAMAPiMF_00086 S_AdoMet_synth1, 1 hit
InterProiView protein in InterPro
IPR022631 ADOMET_SYNTHASE_CS
IPR022630 S-AdoMet_synt_C
IPR022629 S-AdoMet_synt_central
IPR022628 S-AdoMet_synt_N
IPR002133 S-AdoMet_synthetase
IPR022636 S-AdoMet_synthetase_sfam
PANTHERiPTHR11964 PTHR11964, 1 hit
PfamiView protein in Pfam
PF02773 S-AdoMet_synt_C, 1 hit
PF02772 S-AdoMet_synt_M, 1 hit
PF00438 S-AdoMet_synt_N, 1 hit
PIRSFiPIRSF000497 MAT, 1 hit
SUPFAMiSSF55973 SSF55973, 3 hits
TIGRFAMsiTIGR01034 metK, 1 hit
PROSITEiView protein in PROSITE
PS00376 ADOMET_SYNTHASE_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q2RK28-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTRKLFTSES VTEGHPDKIA DRIADAVLDA IYAKDNQARV ACECLVSTGL
60 70 80 90 100
ILVAGQITTD CYVDIPRVAR ETVREIGYTR AKFGFDCDTC AVITSIDEQS
110 120 130 140 150
PDIAMGVNEA WEKKQGEARD EVETLGAGDQ GMMFGYATSE TPEFMPMPIA
160 170 180 190 200
LAHALTRRLA AVRKERILPY LRPDGKSQVT VEYEDGRPVR VDTVVISTQH
210 220 230 240 250
RPDIDMATLR DEVLETVIKP VIPAEMLDNR TRYFINPTGR FVIGGPQGDT
260 270 280 290 300
GLTGRKLIVD TYGGMARHGG GALSGKDPTK VDRSAAYAAR YVAKNVVAAG
310 320 330 340 350
LADRCEVQVA YAIGVARPVS ISVETFGTGK VSDDRLVELI RAHFDLRPGA
360 370 380 390
IIRDLDLRRP IYKAVSVYGH FGRPDLDLPW ERLDKVEALR ADAGL
Length:395
Mass (Da):43,283
Last modified:January 24, 2006 - v1
Checksum:iFC737004FB9759AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000232 Genomic DNA Translation: ABC19211.1
RefSeqiWP_011392413.1, NC_007644.1
YP_429754.1, NC_007644.1

Genome annotation databases

EnsemblBacteriaiABC19211; ABC19211; Moth_0894
GeneIDi3831435
KEGGimta:Moth_0894
PATRICifig|264732.11.peg.961

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000232 Genomic DNA Translation: ABC19211.1
RefSeqiWP_011392413.1, NC_007644.1
YP_429754.1, NC_007644.1

3D structure databases

SMRiQ2RK28
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi264732.Moth_0894

Proteomic databases

PRIDEiQ2RK28

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC19211; ABC19211; Moth_0894
GeneIDi3831435
KEGGimta:Moth_0894
PATRICifig|264732.11.peg.961

Phylogenomic databases

eggNOGiENOG4105CPH Bacteria
COG0192 LUCA
HOGENOMiHOG000245710
KOiK00789
OMAiPGHFLFT
OrthoDBiPOG091H00IO

Enzyme and pathway databases

UniPathwayi
UPA00315;UER00080

BioCyciMTHE264732:G1G5J-929-MONOMER

Family and domain databases

HAMAPiMF_00086 S_AdoMet_synth1, 1 hit
InterProiView protein in InterPro
IPR022631 ADOMET_SYNTHASE_CS
IPR022630 S-AdoMet_synt_C
IPR022629 S-AdoMet_synt_central
IPR022628 S-AdoMet_synt_N
IPR002133 S-AdoMet_synthetase
IPR022636 S-AdoMet_synthetase_sfam
PANTHERiPTHR11964 PTHR11964, 1 hit
PfamiView protein in Pfam
PF02773 S-AdoMet_synt_C, 1 hit
PF02772 S-AdoMet_synt_M, 1 hit
PF00438 S-AdoMet_synt_N, 1 hit
PIRSFiPIRSF000497 MAT, 1 hit
SUPFAMiSSF55973 SSF55973, 3 hits
TIGRFAMsiTIGR01034 metK, 1 hit
PROSITEiView protein in PROSITE
PS00376 ADOMET_SYNTHASE_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMETK_MOOTA
AccessioniPrimary (citable) accession number: Q2RK28
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: January 24, 2006
Last modified: October 10, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again