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Protein

Bifunctional NAD(P)H-hydrate repair enzyme Nnr

Gene

nnr

Organism
Moorella thermoacetica (strain ATCC 39073 / JCM 9320)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).By similarity

Catalytic activityi

ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide = AMP + phosphate + NADH.
ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide phosphate = AMP + phosphate + NADPH.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.

Cofactori

K+By similarityNote: Binds 1 potassium ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61PotassiumBy similarity1
Metal bindingi132PotassiumBy similarity1
Binding sitei165NAD(P)HX (for epimerase activity)By similarity1
Metal bindingi168PotassiumBy similarity1
Binding sitei338NAD(P)HX (for dehydratase activity); via amide nitrogenBy similarity1
Binding sitei456NAD(P)HX (for dehydratase activity)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi426 – 430ADPBy similarity5
Nucleotide bindingi446 – 455ADPBy similarity10

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme
LigandATP-binding, Metal-binding, NAD, NADP, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciMTHE264732:G1G5J-2246-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional NAD(P)H-hydrate repair enzyme Nnr
Alternative name(s):
Nicotinamide nucleotide repair protein
Including the following 2 domains:
ADP-dependent (S)-NAD(P)H-hydrate dehydratase (EC:4.2.1.136)
Alternative name(s):
ADP-dependent NAD(P)HX dehydratase
NAD(P)H-hydrate epimerase (EC:5.1.99.6)
Alternative name(s):
NAD(P)HX epimerase
Gene namesi
Name:nnr
Ordered Locus Names:Moth_2168
OrganismiMoorella thermoacetica (strain ATCC 39073 / JCM 9320)
Taxonomic identifieri264732 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeMoorella groupMoorella
Proteomesi
  • UP000007053 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004164191 – 530Bifunctional NAD(P)H-hydrate repair enzyme NnrAdd BLAST530

Interactioni

Protein-protein interaction databases

STRINGi264732.Moth_2168

Structurei

3D structure databases

ProteinModelPortaliQ2RGI2
SMRiQ2RGI2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 222YjeF N-terminalAdd BLAST214
Domaini232 – 515YjeF C-terminalAdd BLAST284

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 224NAD(P)H-hydrate epimeraseBy similarityAdd BLAST224
Regioni60 – 64NAD(P)HX (for epimerase activity)By similarity5
Regioni136 – 142NAD(P)HX (for epimerase activity)By similarity7
Regioni232 – 530ADP-dependent (S)-NAD(P)H-hydrate dehydrataseBy similarityAdd BLAST299
Regioni389 – 395NAD(P)HX (for dehydratase activity)By similarity7

Sequence similaritiesi

In the N-terminal section; belongs to the NnrE/AIBP family.Curated
In the C-terminal section; belongs to the NnrD/CARKD family.Curated

Phylogenomic databases

eggNOGiENOG4105DR1 Bacteria
COG0062 LUCA
COG0063 LUCA
HOGENOMiHOG000228404
KOiK17758
K17759
OMAiLVGPGHN
OrthoDBiPOG091H01XZ

Family and domain databases

CDDicd01171 YXKO-related, 1 hit
Gene3Di3.40.1190.20, 1 hit
3.40.50.10260, 1 hit
HAMAPiMF_01965 NADHX_dehydratase, 1 hit
MF_01966 NADHX_epimerase, 1 hit
InterProiView protein in InterPro
IPR017953 Carbohydrate_kinase_pred_CS
IPR000631 CARKD
IPR030677 Nnr
IPR029056 Ribokinase-like
IPR004443 YjeF_N_dom
IPR036652 YjeF_N_dom_sf
PfamiView protein in Pfam
PF01256 Carb_kinase, 1 hit
PF03853 YjeF_N, 1 hit
PIRSFiPIRSF017184 Nnr, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
SSF64153 SSF64153, 1 hit
TIGRFAMsiTIGR00196 yjeF_cterm, 1 hit
TIGR00197 yjeF_nterm, 1 hit
PROSITEiView protein in PROSITE
PS01050 YJEF_C_2, 1 hit
PS51383 YJEF_C_3, 1 hit
PS51385 YJEF_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q2RGI2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MYLVTAAEMG QLDRLASSEY MIPSIVLMEN AGLRVVESIE RHFQGQVANR
60 70 80 90 100
RILIFCGKGN NGGDGLVVAR HLLNRGAEVK VFLLARPEDI RGDARTNLEI
110 120 130 140 150
YQKMGGKLLL LLGESHLQRA DIALLYADLV VDAIFGTGFK GAAMGLPAAV
160 170 180 190 200
INMINKAHRE TVAVDLPSGL EADTGRCFGP CIQATWTVTF ALPKLGLVVE
210 220 230 240 250
PGASLTGRLE VADIGIPQKL VATQHFNRRL LTAAWCRSQL PRREASGHKG
260 270 280 290 300
LYGRVLAVGG SPGLTGAITL AATAALKAGA GLVTAAVPRG VQGILAMKTT
310 320 330 340 350
EIMTMSLPET PAGALSRDAL DPLLERLAEV DVLAIGPGLS RDPATVDLVK
360 370 380 390 400
ELLPRVQVPA VVDADALNAL ATDTRVLTGD HGPLVLTPHP GEMARLLGTT
410 420 430 440 450
AAKIQEDRLE IAAKYAREWQ AVLLLKGART VIAWPDGQVY INPTGNPGMA
460 470 480 490 500
TAGSGDVLTG IIAGLAGQGL KPGVAAALGA YLHGAAGDEA ARQRGQRAMM
510 520 530
AGDLLDFLPY VLRNLEEEVE TIVAAGLGRD
Length:530
Mass (Da):55,712
Last modified:January 24, 2006 - v1
Checksum:iCF57131AF561867C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000232 Genomic DNA Translation: ABC20457.1
RefSeqiWP_011393655.1, NC_007644.1
YP_431000.1, NC_007644.1

Genome annotation databases

EnsemblBacteriaiABC20457; ABC20457; Moth_2168
GeneIDi3833017
KEGGimta:Moth_2168
PATRICifig|264732.11.peg.2362

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000232 Genomic DNA Translation: ABC20457.1
RefSeqiWP_011393655.1, NC_007644.1
YP_431000.1, NC_007644.1

3D structure databases

ProteinModelPortaliQ2RGI2
SMRiQ2RGI2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi264732.Moth_2168

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC20457; ABC20457; Moth_2168
GeneIDi3833017
KEGGimta:Moth_2168
PATRICifig|264732.11.peg.2362

Phylogenomic databases

eggNOGiENOG4105DR1 Bacteria
COG0062 LUCA
COG0063 LUCA
HOGENOMiHOG000228404
KOiK17758
K17759
OMAiLVGPGHN
OrthoDBiPOG091H01XZ

Enzyme and pathway databases

BioCyciMTHE264732:G1G5J-2246-MONOMER

Family and domain databases

CDDicd01171 YXKO-related, 1 hit
Gene3Di3.40.1190.20, 1 hit
3.40.50.10260, 1 hit
HAMAPiMF_01965 NADHX_dehydratase, 1 hit
MF_01966 NADHX_epimerase, 1 hit
InterProiView protein in InterPro
IPR017953 Carbohydrate_kinase_pred_CS
IPR000631 CARKD
IPR030677 Nnr
IPR029056 Ribokinase-like
IPR004443 YjeF_N_dom
IPR036652 YjeF_N_dom_sf
PfamiView protein in Pfam
PF01256 Carb_kinase, 1 hit
PF03853 YjeF_N, 1 hit
PIRSFiPIRSF017184 Nnr, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
SSF64153 SSF64153, 1 hit
TIGRFAMsiTIGR00196 yjeF_cterm, 1 hit
TIGR00197 yjeF_nterm, 1 hit
PROSITEiView protein in PROSITE
PS01050 YJEF_C_2, 1 hit
PS51383 YJEF_C_3, 1 hit
PS51385 YJEF_N, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNNR_MOOTA
AccessioniPrimary (citable) accession number: Q2RGI2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: January 24, 2006
Last modified: October 10, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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