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Entry version 68 (11 Dec 2019)
Sequence version 1 (07 Feb 2006)
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Protein

Thiol-activated cytolysin

Gene

samhpaf

Organism
Streptococcus mitis
Status
Unreviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi68Calcium 1; via carbonyl oxygenCombined sources1
Metal bindingi68Calcium 2; via carbonyl oxygenCombined sources1
Metal bindingi71Calcium 1Combined sources1
Metal bindingi73Calcium 1; via carbonyl oxygenCombined sources1
Metal bindingi73Calcium 2; via carbonyl oxygenCombined sources1
Metal bindingi81Magnesium; via tele nitrogenCombined sources1
Metal bindingi82Calcium 2Combined sources1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei85FucoseCombined sources1
Binding sitei112FucoseCombined sources1
Binding sitei120FucoseCombined sources1
Metal bindingi176Calcium 1; via carbonyl oxygenCombined sources1
Metal bindingi176Calcium 2; via carbonyl oxygenCombined sources1
Metal bindingi177Calcium 1Combined sources1
Metal bindingi177Calcium 2Combined sources1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionToxinUniRule annotation
Biological processCytolysis, HemolysisUniRule annotation
LigandCalciumCombined sources, Lipid-bindingUniRule annotation, MagnesiumCombined sources, Metal-bindingCombined sources

Protein family/group databases

Carbohydrate-Active enZymes

More...
CAZyi
CBM47 Carbohydrate-Binding Module Family 47

UniLectin database of carbohydrate-binding proteins

More...
UniLectini
Q2PHL4

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Thiol-activated cytolysinUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:samhpafImported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiStreptococcus mitisImported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri28037 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membraneUniRule annotation, Host membrane, Membrane, SecretedUniRule annotation

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section denotes the presence of an N-terminal signal peptide.<p><a href='/help/signal' target='_top'>More...</a></p>Signal peptidei1 – 36UniRule annotationAdd BLAST36
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_500973766437 – 665Thiol-activated cytolysinUniRule annotationAdd BLAST629

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q2PHL4

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
Q2PHL4

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini30 – 183F5/8 type CInterPro annotationAdd BLAST154

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the thiol-activated cytolysin family.UniRule annotation

Keywords - Domaini

SignalUniRule annotation, Transmembrane, Transmembrane beta strandUniRule annotation

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105E5P Bacteria
ENOG410XQPX LUCA

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.60.120.260, 1 hit
2.60.40.1430, 1 hit
3.90.840.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR000421 FA58C
IPR006585 FTP1
IPR008979 Galactose-bd-like_sf
IPR035390 Thiol_cytolys_C
IPR038700 Thiol_cytolys_C_sf
IPR001869 Thiol_cytolysin
IPR036363 Thiol_cytolysin_ab_sf
IPR036359 Thiol_cytolysin_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00754 F5_F8_type_C, 1 hit
PF17440 Thiol_cytolys_C, 1 hit
PF01289 Thiol_cytolysin, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR01400 TACYTOLYSIN

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00607 FTP, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF49785 SSF49785, 1 hit
SSF56978 SSF56978, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50022 FA58C_3, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q2PHL4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNQEKRLHRF VKKCGLGVCS AVVAAFLLNA QGVALATEQG NRPVETENIA
60 70 80 90 100
RGKQASQSST AYGGAAARAV DGNVDSDYGH HSVTHTNFED NAWWQVDLGK
110 120 130 140 150
TENVGKVKLY NRGDGNVANR LSNFDVVLLN EAKQEVARQH FDSLNGKAEL
160 170 180 190 200
EVFFTAKAAR YVKVELKTKN TPLSLAEVEV FRSATTQVGQ DRTAPVVDQT
210 220 230 240 250
SALKDYLFGL AYNPLDILTR KGETLENRYN TSAKEQNGEF VVVEKIKKTL
260 270 280 290 300
STGTADVSIN GNQNVFLGGL YKANQNLLEN QPELISLARA KGTVSVDLPG
310 320 330 340 350
MIQSDSRIEA DPTTSGMQSA MNTLVERWTK NYSSSHSVPA RVQYESTTAY
360 370 380 390 400
SMNQLKAKFG ADFEKAGAPL KIDFEAVQKG EKQIEVVNFK QIYYTATFDA
410 420 430 440 450
PTNPAAVFDK SVTPEDLKQR GVDSQTPPVY VSNVSYGRQI YVKFESASKS
460 470 480 490 500
TELKAAINAV IKGATIAPNS EWSRLLKNTS VTAVIVGGNA SGAAKVVTGT
510 520 530 540 550
VENLKELIRE GANFSAQSPA VPISYKTAFL KDNAQATLQN STDYIETKVT
560 570 580 590 600
SYKNGFLKLH HKGAYVARYY IYWDEITYDE QGNPEIRSRQ WEDNGKNRTS
610 620 630 640 650
GFQTEIQFRG NVRNLRIKVQ EKTGLVWEPW RTVYNRTDLP LVQQRTITHW
660
GTTLNPKVDE KIVNE
Length:665
Mass (Da):73,598
Last modified:February 7, 2006 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iC5ED3F30AA91BA96
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AB051299 Genomic DNA Translation: BAE72438.1

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB051299 Genomic DNA Translation: BAE72438.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LE0X-ray1.91A38-190[»]
3LEGX-ray2.01A38-190[»]
3LEIX-ray1.90A38-190[»]
3LEKX-ray2.00A38-190[»]
4GWIX-ray1.60A38-190[»]
4GWJX-ray1.60A38-190[»]
SMRiQ2PHL4
ModBaseiSearch...
PDBe-KBiSearch...

Protein family/group databases

CAZyiCBM47 Carbohydrate-Binding Module Family 47
UniLectiniQ2PHL4

Phylogenomic databases

eggNOGiENOG4105E5P Bacteria
ENOG410XQPX LUCA

Miscellaneous databases

EvolutionaryTraceiQ2PHL4

Family and domain databases

Gene3Di2.60.120.260, 1 hit
2.60.40.1430, 1 hit
3.90.840.10, 1 hit
InterProiView protein in InterPro
IPR000421 FA58C
IPR006585 FTP1
IPR008979 Galactose-bd-like_sf
IPR035390 Thiol_cytolys_C
IPR038700 Thiol_cytolys_C_sf
IPR001869 Thiol_cytolysin
IPR036363 Thiol_cytolysin_ab_sf
IPR036359 Thiol_cytolysin_sf
PfamiView protein in Pfam
PF00754 F5_F8_type_C, 1 hit
PF17440 Thiol_cytolys_C, 1 hit
PF01289 Thiol_cytolysin, 1 hit
PRINTSiPR01400 TACYTOLYSIN
SMARTiView protein in SMART
SM00607 FTP, 1 hit
SUPFAMiSSF49785 SSF49785, 1 hit
SSF56978 SSF56978, 1 hit
PROSITEiView protein in PROSITE
PS50022 FA58C_3, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiQ2PHL4_STRMT
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q2PHL4
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/TrEMBL: February 7, 2006
Last sequence update: February 7, 2006
Last modified: December 11, 2019
This is version 68 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiUnreviewed (UniProtKB/TrEMBL)

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources
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