UniProtKB - Q2NL30 (SUV91_BOVIN)
Histone-lysine N-methyltransferase SUV39H1
SUV39H1
Functioni
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation (By similarity).
By similarityCatalytic activityi
- L-lysyl9-[histone H3] + 3 S-adenosyl-L-methionine = 3 H+ + N6,N6,N6-trimethyl-L-lysyl9-[histone H3] + 3 S-adenosyl-L-homocysteinePROSITE-ProRule annotationBy similarityEC:2.1.1.355PROSITE-ProRule annotationBy similarity
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 181 | Zinc 1By similarity | 1 | |
Metal bindingi | 181 | Zinc 2By similarity | 1 | |
Metal bindingi | 183 | Zinc 1By similarity | 1 | |
Metal bindingi | 186 | Zinc 1By similarity | 1 | |
Metal bindingi | 186 | Zinc 3By similarity | 1 | |
Metal bindingi | 194 | Zinc 1By similarity | 1 | |
Metal bindingi | 195 | Zinc 1By similarity | 1 | |
Metal bindingi | 195 | Zinc 2By similarity | 1 | |
Metal bindingi | 222 | Zinc 2By similarity | 1 | |
Metal bindingi | 222 | Zinc 3By similarity | 1 | |
Metal bindingi | 226 | Zinc 2By similarity | 1 | |
Metal bindingi | 228 | Zinc 3By similarity | 1 | |
Metal bindingi | 232 | Zinc 3By similarity | 1 | |
Binding sitei | 297 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Metal bindingi | 326 | Zinc 4By similarity | 1 | |
Metal bindingi | 400 | Zinc 4By similarity | 1 | |
Metal bindingi | 402 | Zinc 4By similarity | 1 | |
Metal bindingi | 407 | Zinc 4By similarity | 1 |
GO - Molecular functioni
- histone methyltransferase activity (H3-K9 specific) Source: UniProtKB
- transcription cis-regulatory region binding Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell differentiation Source: UniProtKB-KW
- chromatin organization Source: UniProtKB-KW
- histone H3-K9 dimethylation Source: UniProtKB
- histone H3-K9 trimethylation Source: UniProtKB
- negative regulation of circadian rhythm Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- rhythmic process Source: UniProtKB-KW
- rRNA processing Source: UniProtKB-KW
Keywordsi
Molecular function | Chromatin regulator, Methyltransferase, Repressor, Transferase |
Biological process | Biological rhythms, Cell cycle, Differentiation, rRNA processing, Transcription, Transcription regulation |
Ligand | Metal-binding, S-adenosyl-L-methionine, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Histone-lysine N-methyltransferase SUV39H1 (EC:2.1.1.355By similarity)Alternative name(s): Suppressor of variegation 3-9 homolog 1 Short name: Su(var)3-9 homolog 1 |
Gene namesi | Name:SUV39H1 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Nucleus
- Nucleus By similarity
- Nucleus lamina By similarity
- nucleoplasm By similarity
Other locations
- centromere By similarity
Note: Associates with centromeric constitutive heterochromatin.By similarity
Nucleus
- nuclear lamina Source: UniProtKB-SubCell
- nucleoplasm Source: UniProtKB-SubCell
- nucleus Source: UniProtKB
Other locations
- chromosome, centromeric region Source: UniProtKB-SubCell
- heterochromatin Source: UniProtKB
Keywords - Cellular componenti
Centromere, Chromosome, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000281809 | 1 – 412 | Histone-lysine N-methyltransferase SUV39H1Add BLAST | 412 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 266 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 391 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | Q2NL30 |
PRIDEi | Q2NL30 |
Interactioni
Subunit structurei
Interacts with H3 and H4 histones.
Interacts with GFI1B, DNMT3B, CBX1, CBX4, CCAR2, MBD1, RUNX1, RUNX3, MYOD1, SMAD5 and RB1.
Interacts with SBF1 through the SET domain.
Interacts with HDAC1 and HDAC2 through the N-terminus and associates with the core histone deacetylase complex composed of HDAC1, HDAC2, RBBP4 and RBBP7.
Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8 (By similarity).
Interacts (via SET domain) with MECOM; enhances MECOM transcriptional repression activity.
Interacts with LMNA; the interaction increases stability of SUV39H1. The large PER complex involved in the histone methylation is composed of at least PER2, CBX3, TRIM28, SUV39H1 and/or SUV39H2; CBX3 mediates the formation of the complex (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000006178 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 43 – 101 | ChromoPROSITE-ProRule annotationAdd BLAST | 59 | |
Domaini | 179 – 240 | Pre-SETPROSITE-ProRule annotationAdd BLAST | 62 | |
Domaini | 243 – 366 | SETPROSITE-ProRule annotationAdd BLAST | 124 | |
Domaini | 396 – 412 | Post-SETPROSITE-ProRule annotationAdd BLAST | 17 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 89 | Interaction with SIRT1By similarityAdd BLAST | 89 | |
Regioni | 254 – 256 | S-adenosyl-L-methionine bindingBy similarity | 3 | |
Regioni | 255 – 377 | Mediates interaction with MECOMBy similarityAdd BLAST | 123 | |
Regioni | 323 – 324 | S-adenosyl-L-methionine bindingBy similarity | 2 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1082, Eukaryota |
InParanoidi | Q2NL30 |
OrthoDBi | 753093at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR016197, Chromo-like_dom_sf IPR000953, Chromo/chromo_shadow_dom IPR023780, Chromo_domain IPR023779, Chromodomain_CS IPR011381, Histone_H3-K9_MeTrfase IPR003616, Post-SET_dom IPR007728, Pre-SET_dom IPR001214, SET_dom |
Pfami | View protein in Pfam PF00385, Chromo, 1 hit PF05033, Pre-SET, 1 hit PF00856, SET, 1 hit |
PIRSFi | PIRSF009343, SUV39_SET, 1 hit |
SMARTi | View protein in SMART SM00298, CHROMO, 1 hit SM00508, PostSET, 1 hit SM00468, PreSET, 1 hit SM00317, SET, 1 hit |
SUPFAMi | SSF54160, SSF54160, 1 hit |
PROSITEi | View protein in PROSITE PS00598, CHROMO_1, 1 hit PS50013, CHROMO_2, 1 hit PS50868, POST_SET, 1 hit PS50867, PRE_SET, 1 hit PS51579, SAM_MT43_SUVAR39_3, 1 hit PS50280, SET, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAESLKGCSV CCKSSWNQLQ DLCRLAKLSC PALGISKRNL YDFEVEYLCD
60 70 80 90 100
YKKIREQEYY LVKWRGYPDS ESTWEPRQNL KCVRILKQFH KDLERELLRR
110 120 130 140 150
HHRSKPPRHL DPSLANYLVQ KAKQRRALRR WEQELNAKRS HLGRITVENE
160 170 180 190 200
VDLDGPPRAF VYINEYRVGE GITLNQVAVG CECQDCLWAP AGGCCPGASL
210 220 230 240 250
HKFAYNDQGQ VRLRAGLPIY ECNSRCRCGY DCPNRVVQKG IRYDLCIFRT
260 270 280 290 300
DDGRGWGVRT LEKIRKNSFV MEYVGEIITS EEAERRGQIY DRQGATYLFD
310 320 330 340 350
LDYVEDVYTV DAAYYGNISH FVNHSCDPNL QVYNVFIDNL DERLPRIAFF
360 370 380 390 400
ATRTIRAGEE LTFDYNMQVD PVDMESTRMD SNFGLAGLPG SPKKRVRIEC
410
KCGTESCRKY LF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC111156 mRNA Translation: AAI11157.1 |
RefSeqi | NP_001039729.1, NM_001046264.2 |
Genome annotation databases
GeneIDi | 523047 |
KEGGi | bta:523047 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC111156 mRNA Translation: AAI11157.1 |
RefSeqi | NP_001039729.1, NM_001046264.2 |
3D structure databases
SMRi | Q2NL30 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000006178 |
Proteomic databases
PaxDbi | Q2NL30 |
PRIDEi | Q2NL30 |
Genome annotation databases
GeneIDi | 523047 |
KEGGi | bta:523047 |
Organism-specific databases
CTDi | 6839 |
Phylogenomic databases
eggNOGi | KOG1082, Eukaryota |
InParanoidi | Q2NL30 |
OrthoDBi | 753093at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR016197, Chromo-like_dom_sf IPR000953, Chromo/chromo_shadow_dom IPR023780, Chromo_domain IPR023779, Chromodomain_CS IPR011381, Histone_H3-K9_MeTrfase IPR003616, Post-SET_dom IPR007728, Pre-SET_dom IPR001214, SET_dom |
Pfami | View protein in Pfam PF00385, Chromo, 1 hit PF05033, Pre-SET, 1 hit PF00856, SET, 1 hit |
PIRSFi | PIRSF009343, SUV39_SET, 1 hit |
SMARTi | View protein in SMART SM00298, CHROMO, 1 hit SM00508, PostSET, 1 hit SM00468, PreSET, 1 hit SM00317, SET, 1 hit |
SUPFAMi | SSF54160, SSF54160, 1 hit |
PROSITEi | View protein in PROSITE PS00598, CHROMO_1, 1 hit PS50013, CHROMO_2, 1 hit PS50868, POST_SET, 1 hit PS50867, PRE_SET, 1 hit PS51579, SAM_MT43_SUVAR39_3, 1 hit PS50280, SET, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SUV91_BOVIN | |
Accessioni | Q2NL30Primary (citable) accession number: Q2NL30 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2007 |
Last sequence update: | February 7, 2006 | |
Last modified: | February 23, 2022 | |
This is version 103 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families