UniProtKB - Q2NKY8 (DHX30_BOVIN)
Protein
ATP-dependent RNA helicase DHX30
Gene
DHX30
Organism
Bos taurus (Bovine)
Status
Functioni
RNA dependent helicase. Plays an important role in the assembly of the mitochondrial large ribosomal subunit. Required for optimal function of the zinc-finger antiviral protein ZC3HAV1. Associates with mitochondrial DNA. Involved in nervous system development and differentiation through its involvement in the up-regulation of a number of genes which are required for neurogenesis, including GSC, NCAM1, neurogenin, and NEUROD.By similarity
Catalytic activityi
- EC:3.6.4.13By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 483 – 490 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- chromatin binding Source: UniProtKB
- RNA binding Source: UniProtKB
- RNA helicase activity Source: UniProtKB
GO - Biological processi
- central nervous system development Source: UniProtKB
- mitochondrial large ribosomal subunit assembly Source: UniProtKB
Keywordsi
Molecular function | Helicase, Hydrolase, RNA-binding |
Biological process | Ribosome biogenesis |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent RNA helicase DHX30Curated (EC:3.6.4.13By similarity)Alternative name(s): DEAH box protein 30 |
Gene namesi | Name:DHX30 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
- mitochondrion nucleoid By similarity
Other locations
- Cytoplasm By similarity
Note: Localizes to mitochondrial RNA granules found in close proximity to the mitochondrial nucleoids. Relocalizes to stress granules upon heat stress.By similarity
Mitochondrion
- mitochondrial nucleoid Source: UniProtKB
- mitochondrion Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- ribonucleoprotein granule Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Mitochondrion, Mitochondrion nucleoidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000245537 | 1 – 1220 | ATP-dependent RNA helicase DHX30Add BLAST | 1220 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 252 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q2NKY8 |
PRIDEi | Q2NKY8 |
Interactioni
Subunit structurei
Identified in a complex with TFAM and SSBP1.
Interacts with AGO1 and AGO2 (By similarity).
Interacts (via N-terminus) with ZC3HAV1 (via N-terminal domain) in an RNA-independent manner.
Found in a complex with GRSF1, DDX28, FASTKD2 and FASTKD5.
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000030598 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 79 – 147 | DRBMAdd BLAST | 69 | |
Domaini | 470 – 638 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 169 | |
Domaini | 680 – 853 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 174 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 585 – 588 | DEAH box | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 214 – 225 | Poly-GluAdd BLAST | 12 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0920, Eukaryota |
InParanoidi | Q2NKY8 |
OrthoDBi | 278674at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR011709, DUF1605 IPR007502, Helicase-assoc_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF04408, HA2, 1 hit PF00271, Helicase_C, 1 hit PF07717, OB_NTP_bind, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00847, HA2, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
i Sequence
Sequence statusi: Complete.
Q2NKY8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATARRLMAL AAGVSPRLRP LGPRAIGRQG GPRGLSTGCS RPDRTQEAAE
60 70 80 90 100
AEAAPGEPGE GDGSVVNASR DLLKEFPQPK NLLNSVIGRA LGISHAKDKL
110 120 130 140 150
VYVHTNGPKK KKVTLHIKWP KSVEVEGYGS KKIDAERQAA AAACQLFKGW
160 170 180 190 200
GLLGPRNELF DAAKYRVLAD RFGSPADSWW RPEPTMPPTS WRQLNPESIR
210 220 230 240 250
PGGPGGLSRS LGREEEEDEE EELEEGTIDV TEFLSMTQQD SHAPLRDSRG
260 270 280 290 300
GSFEMTDDDS AIRALTQFPL PKNLLAKVIQ IATSSSTAKN LMQFHTVGTK
310 320 330 340 350
TKLSTLTLLW PCPMTFVAKG RRKAEAENKA AALACNKLKS LGLVDRNNEP
360 370 380 390 400
LTHAMYNLAS LRELGETQRR PCTIQVPEPI LRKIETFLNH YPVESSWISS
410 420 430 440 450
ELRLQGEDIL PLGKDSGPLS DPITGKPYVP LSEAEELRLS QSLLELWRRR
460 470 480 490 500
GPVWQEAPQL PVDPHRDTIL NAIEQHPVVV IAGDTGCGKT TRIPQLLLER
510 520 530 540 550
YVTEGRGARC NVIITQPRRI SAVSVAQRVS HELGPTLRRN VGFQVRLESK
560 570 580 590 600
PPARGGALLF CTVGILLRKL QSNPSLEGVS HVVVDEEHER DVNTDFLLIL
610 620 630 640 650
LKGLQRLNPA LRLVLMSATG DNERFSRYFG GCPVIKVPGF MYPVKEHYLE
660 670 680 690 700
DILAKLGKHQ YPHRHRHHES EDECALDLDL VTDLVLHIDA RGEPGGILCF
710 720 730 740 750
LPGWQEIKGV QQRLQEALGM HESKYLILPV HSNIPMMDQK AIFQQPPIGV
760 770 780 790 800
RKIVLATNIA ETSITINDIV HVVDSGLHKE ERYDLKTKVS CLETVWVSRA
810 820 830 840 850
NVIQRRGRAG RCQSGFAYHL FPRSRLEKMA PFQVPEILRT PLENLVLQAK
860 870 880 890 900
IHMPEKTAVE FLSKAVDSPN IKAVDEAVIL LQEIGVLDQR EYLTTLGQRL
910 920 930 940 950
AHISTDPRLA KAIVLAAIFR CLHPLLVVVS CLTRDPFSSS LQNRAEVDKV
960 970 980 990 1000
KALLSHDSGS DHLAFVRAVS GWEEVLRWQD RSSRENYLEE NLLYAPSLRF
1010 1020 1030 1040 1050
IHGLIKQFSE NIYEAFLVGK PSDCTLASAQ CNEYSEEEEL VKGVLMAGLY
1060 1070 1080 1090 1100
PNLIQVRQGK VTRQGKFKPN SVTYRTKSGN ILLHKSTINR EATRLRSRWL
1110 1120 1130 1140 1150
TYFMAVKSNG SVFVRDSSQV HPLAVLLLTD GDVHIRDDGR RATISLSDSD
1160 1170 1180 1190 1200
LLRLEGDSRT VRLLRELRRA LGRMVERSLR SELAALPPCV QEEHGQLLAL
1210 1220
LAELLRGPCG SFDVRKTADD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC111349 mRNA Translation: AAI11350.1 |
RefSeqi | NP_001070003.1, NM_001076535.2 |
Genome annotation databases
GeneIDi | 767607 |
KEGGi | bta:767607 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC111349 mRNA Translation: AAI11350.1 |
RefSeqi | NP_001070003.1, NM_001076535.2 |
3D structure databases
SMRi | Q2NKY8 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000030598 |
Proteomic databases
PaxDbi | Q2NKY8 |
PRIDEi | Q2NKY8 |
Genome annotation databases
GeneIDi | 767607 |
KEGGi | bta:767607 |
Organism-specific databases
CTDi | 22907 |
Phylogenomic databases
eggNOGi | KOG0920, Eukaryota |
InParanoidi | Q2NKY8 |
OrthoDBi | 278674at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR011709, DUF1605 IPR007502, Helicase-assoc_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF04408, HA2, 1 hit PF00271, Helicase_C, 1 hit PF07717, OB_NTP_bind, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00847, HA2, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DHX30_BOVIN | |
Accessioni | Q2NKY8Primary (citable) accession number: Q2NKY8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2006 |
Last sequence update: | February 7, 2006 | |
Last modified: | December 2, 2020 | |
This is version 88 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families