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Protein

LL-diaminopimelate aminotransferase

Gene

dapL

Organism
Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL-diaminopimelate.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate + 2-oxoglutarate = (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + L-glutamate + H2O.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (aminotransferase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. LL-diaminopimelate aminotransferase (dapL)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (aminotransferase route), the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei16SubstrateUniRule annotation1
Binding sitei43Substrate; via amide nitrogenUniRule annotation1
Binding sitei73Pyridoxal phosphateUniRule annotation1
Binding sitei110SubstrateUniRule annotation1
Binding sitei133Pyridoxal phosphateUniRule annotation1
Binding sitei133SubstrateUniRule annotation1
Binding sitei188Pyridoxal phosphateUniRule annotation1
Binding sitei188SubstrateUniRule annotation1
Binding sitei219Pyridoxal phosphateUniRule annotation1
Binding sitei258Pyridoxal phosphateUniRule annotation1
Binding sitei293Pyridoxal phosphateUniRule annotation1
Binding sitei293SubstrateUniRule annotation1
Binding sitei389SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase
LigandPyridoxal phosphate

Enzyme and pathway databases

BRENDAi2.6.1.83 10175
UniPathwayi
UPA00034;UER00466

Names & Taxonomyi

Protein namesi
Recommended name:
LL-diaminopimelate aminotransferaseUniRule annotation (EC:2.6.1.83UniRule annotation)
Short name:
DAP-ATUniRule annotation
Short name:
DAP-aminotransferaseUniRule annotation
Short name:
LL-DAP-aminotransferaseUniRule annotation
Gene namesi
Name:dapLUniRule annotation
Ordered Locus Names:Msp_0924
OrganismiMethanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)
Taxonomic identifieri339860 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomada groupMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanosphaera
Proteomesi
  • UP000001931 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003422581 – 411LL-diaminopimelate aminotransferaseAdd BLAST411

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei250N6-(pyridoxal phosphate)lysineUniRule annotation1

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi339860.Msp_0924

Structurei

3D structure databases

ProteinModelPortaliQ2NFU1
SMRiQ2NFU1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni109 – 110Pyridoxal phosphate bindingUniRule annotation2
Regioni247 – 249Pyridoxal phosphate bindingUniRule annotation3

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG01133 Archaea
COG0436 LUCA
HOGENOMiHOG000223061
KOiK10206
OMAiTGTRCAF
OrthoDBiPOG093Z02MQ

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
HAMAPiMF_01642 DapL_aminotrans_1, 1 hit
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR019942 DapL/ALD1
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR43144 PTHR43144, 1 hit
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR03542 DAPAT_plant, 1 hit

Sequencei

Sequence statusi: Complete.

Q2NFU1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAVKVNEYYS LIQNNYLFVE IARRVDEYQK ENPDANLIKM GIGDVTRPLA
60 70 80 90 100
KSVVEAFKRA VDELGDADTF RGYGPEQGYD FLIEDVIEND YKPLGVELKN
110 120 130 140 150
SEVFISDGAK CDTGNFQELF SKDNIIAVTD PVYPVYVDSN VMAGRSGKMG
160 170 180 190 200
EDGFYENIVY LPATSENDFV PSLPSEKVDI IYLCYPNNPT GTTLTKEQLK
210 220 230 240 250
EWVDYAHEND ALILFDAAYE SFIKTPGIPH SIFEIEGAKD VAVEFRSYSK
260 270 280 290 300
VAGFTGTRCA YCVVPEEVYV KDANGNKVQL NPLWNRRQST KFNGVSYPVQ
310 320 330 340 350
RAAQAIYTPE GKKEIQENLD YYVKNAQVIR ESLEKMGLKV YGGVDSPYIW
360 370 380 390 400
FKTPNDIDSW SFFDILLKEA HVVSTPGAGF GPSGEGYLRL TAFNTYENTV
410
EAMDRISKLE F
Length:411
Mass (Da):46,292
Last modified:February 7, 2006 - v1
Checksum:i4B70F8067EA97C49
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000102 Genomic DNA Translation: ABC57312.1
RefSeqiWP_011406511.1, NC_007681.1

Genome annotation databases

EnsemblBacteriaiABC57312; ABC57312; Msp_0924
GeneIDi3856231
KEGGimst:Msp_0924

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000102 Genomic DNA Translation: ABC57312.1
RefSeqiWP_011406511.1, NC_007681.1

3D structure databases

ProteinModelPortaliQ2NFU1
SMRiQ2NFU1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi339860.Msp_0924

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC57312; ABC57312; Msp_0924
GeneIDi3856231
KEGGimst:Msp_0924

Phylogenomic databases

eggNOGiarCOG01133 Archaea
COG0436 LUCA
HOGENOMiHOG000223061
KOiK10206
OMAiTGTRCAF
OrthoDBiPOG093Z02MQ

Enzyme and pathway databases

UniPathwayi
UPA00034;UER00466

BRENDAi2.6.1.83 10175

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
HAMAPiMF_01642 DapL_aminotrans_1, 1 hit
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR019942 DapL/ALD1
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR43144 PTHR43144, 1 hit
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR03542 DAPAT_plant, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDAPAT_METST
AccessioniPrimary (citable) accession number: Q2NFU1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: February 7, 2006
Last modified: October 10, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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