UniProtKB - Q2NEH1 (GATD_METST)
Protein
Glutamyl-tRNA(Gln) amidotransferase subunit D
Gene
gatD
Organism
Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)
Status
Functioni
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.UniRule annotation
Catalytic activityi
- ATP + H2O + L-glutamine + L-glutamyl-tRNAGln = ADP + H+ + L-glutamate + L-glutaminyl-tRNAGln + phosphateUniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 101 | UniRule annotation | 1 | |
Active sitei | 177 | UniRule annotation | 1 | |
Active sitei | 178 | UniRule annotation | 1 | |
Active sitei | 254 | UniRule annotation | 1 |
GO - Molecular functioni
- asparaginase activity Source: InterPro
- ATP binding Source: UniProtKB-KW
- glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Source: UniProtKB-UniRule
GO - Biological processi
- cellular amino acid metabolic process Source: InterPro
- regulation of translational fidelity Source: InterPro
- translation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MSTA339860:G1G4W-1413-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamyl-tRNA(Gln) amidotransferase subunit DUniRule annotation (EC:6.3.5.-UniRule annotation)Short name: Glu-ADT subunit DUniRule annotation |
Gene namesi | Name:gatDUniRule annotation Ordered Locus Names:Msp_1411 |
Organismi | Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3) |
Taxonomic identifieri | 339860 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Methanomada group › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000025460 | 1 – 438 | Glutamyl-tRNA(Gln) amidotransferase subunit DAdd BLAST | 438 |
Interactioni
Subunit structurei
Heterodimer of GatD and GatE.
UniRule annotationProtein-protein interaction databases
STRINGi | 339860.Msp_1411 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 91 – 421 | Asparaginase/glutaminasePROSITE-ProRule annotationAdd BLAST | 331 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | arCOG01924, Archaea |
HOGENOMi | CLU_019134_2_1_2 |
OMAi | VRKNHTS |
OrthoDBi | 61613at2157 |
Family and domain databases
CDDi | cd08962, GatD, 1 hit |
Gene3Di | 2.30.30.520, 1 hit 3.40.50.1170, 1 hit 3.40.50.40, 1 hit |
HAMAPi | MF_00586, GatD, 1 hit |
InterProi | View protein in InterPro IPR006033, AsnA_fam IPR036152, Asp/glu_Ase-like_sf IPR006034, Asparaginase/glutaminase-like IPR027475, Asparaginase/glutaminase_AS2 IPR040919, Asparaginase_C IPR011878, GatD IPR040918, GatD_N IPR037222, GatD_N_sf IPR027473, L-asparaginase_C IPR027474, L-asparaginase_N IPR037152, L-asparaginase_N_sf |
Pfami | View protein in Pfam PF00710, Asparaginase, 1 hit PF17763, Asparaginase_C, 1 hit PF18195, GatD_N, 1 hit |
PIRSFi | PIRSF500175, Glu_ADT_D, 1 hit PIRSF001220, L-ASNase_gatD, 1 hit |
PRINTSi | PR00139, ASNGLNASE |
SMARTi | View protein in SMART SM00870, Asparaginase, 1 hit |
SUPFAMi | SSF141300, SSF141300, 1 hit SSF53774, SSF53774, 1 hit |
TIGRFAMsi | TIGR00519, asnASE_I, 1 hit TIGR02153, gatD_arch, 1 hit |
PROSITEi | View protein in PROSITE PS00144, ASN_GLN_ASE_1, 1 hit PS00917, ASN_GLN_ASE_2, 1 hit PS51732, ASN_GLN_ASE_3, 1 hit |
i Sequence
Sequence statusi: Complete.
Q2NEH1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAYEEYLKDF LEISNITVGD TVKVTKPHIE HEGMLLEKPD YSNENTIILK
60 70 80 90 100
LDSGYNIGID IKDAKIEKIS EGKKPQIELD PVDKKISDKK KNLSILSTGG
110 120 130 140 150
TVASVIDYKT GAVHPAFTAD DLLRATPELV DYANINAKAI FNILSENMTP
160 170 180 190 200
EYWKKTATTI YDEINNGADG IIIAHGTDTM HYTASALSFM IDSPVPIVLT
210 220 230 240 250
GAQRSSDRPS SDAFTNLMAS VNAAKSDIAE VTICMHGTED DSYCDLHRGT
260 270 280 290 300
RARKMHTSRR DTFTSINMNP LARIENNKVT INDTQVKYTK RNEKELSLNT
310 320 330 340 350
NLADKVALIK MYPGISPEII DIYVDKNYDG IVIEGTGLGH CSDDVITSIT
360 370 380 390 400
RATSEDIPVV MTSQCLFGRT NMNVYSSGRR LLQNNVIGVG DMLPETAYTK
410 420 430
LLWAAGQTDN VSEIYEIMKT NLKGEMDTTL SQNYFIKN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000102 Genomic DNA Translation: ABC57782.1 |
RefSeqi | WP_011406981.1, NC_007681.1 |
Genome annotation databases
EnsemblBacteriai | ABC57782; ABC57782; Msp_1411 |
GeneIDi | 41325984 |
KEGGi | mst:Msp_1411 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000102 Genomic DNA Translation: ABC57782.1 |
RefSeqi | WP_011406981.1, NC_007681.1 |
3D structure databases
SMRi | Q2NEH1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 339860.Msp_1411 |
Genome annotation databases
EnsemblBacteriai | ABC57782; ABC57782; Msp_1411 |
GeneIDi | 41325984 |
KEGGi | mst:Msp_1411 |
Phylogenomic databases
eggNOGi | arCOG01924, Archaea |
HOGENOMi | CLU_019134_2_1_2 |
OMAi | VRKNHTS |
OrthoDBi | 61613at2157 |
Enzyme and pathway databases
BioCyci | MSTA339860:G1G4W-1413-MONOMER |
Family and domain databases
CDDi | cd08962, GatD, 1 hit |
Gene3Di | 2.30.30.520, 1 hit 3.40.50.1170, 1 hit 3.40.50.40, 1 hit |
HAMAPi | MF_00586, GatD, 1 hit |
InterProi | View protein in InterPro IPR006033, AsnA_fam IPR036152, Asp/glu_Ase-like_sf IPR006034, Asparaginase/glutaminase-like IPR027475, Asparaginase/glutaminase_AS2 IPR040919, Asparaginase_C IPR011878, GatD IPR040918, GatD_N IPR037222, GatD_N_sf IPR027473, L-asparaginase_C IPR027474, L-asparaginase_N IPR037152, L-asparaginase_N_sf |
Pfami | View protein in Pfam PF00710, Asparaginase, 1 hit PF17763, Asparaginase_C, 1 hit PF18195, GatD_N, 1 hit |
PIRSFi | PIRSF500175, Glu_ADT_D, 1 hit PIRSF001220, L-ASNase_gatD, 1 hit |
PRINTSi | PR00139, ASNGLNASE |
SMARTi | View protein in SMART SM00870, Asparaginase, 1 hit |
SUPFAMi | SSF141300, SSF141300, 1 hit SSF53774, SSF53774, 1 hit |
TIGRFAMsi | TIGR00519, asnASE_I, 1 hit TIGR02153, gatD_arch, 1 hit |
PROSITEi | View protein in PROSITE PS00144, ASN_GLN_ASE_1, 1 hit PS00917, ASN_GLN_ASE_2, 1 hit PS51732, ASN_GLN_ASE_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GATD_METST | |
Accessioni | Q2NEH1Primary (citable) accession number: Q2NEH1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | February 7, 2006 | |
Last modified: | December 2, 2020 | |
This is version 79 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families