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Protein

S-adenosylmethionine synthase

Gene

metK

Organism
Erythrobacter litoralis (strain HTCC2594)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.UniRule annotation

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase (metK)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei16ATPUniRule annotation1
Metal bindingi18MagnesiumUniRule annotation1
Metal bindingi44PotassiumUniRule annotation1
Binding sitei57MethionineUniRule annotation1
Binding sitei110MethionineUniRule annotation1
Binding sitei253ATP; shared with neighboring subunitUniRule annotation1
Binding sitei253Methionine; shared with neighboring subunitUniRule annotation1
Binding sitei276ATP; via amide nitrogen; shared with neighboring subunitUniRule annotation1
Binding sitei280ATP; shared with neighboring subunitUniRule annotation1
Binding sitei284MethionineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi175 – 177ATPUniRule annotation3
Nucleotide bindingi259 – 260ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processOne-carbon metabolism
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciELIT314225:G1G5F-2829-MONOMER
UniPathwayi
UPA00315;UER00080

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthaseUniRule annotation (EC:2.5.1.6UniRule annotation)
Short name:
AdoMet synthaseUniRule annotation
Alternative name(s):
MATUniRule annotation
Methionine adenosyltransferaseUniRule annotation
Gene namesi
Name:metKUniRule annotation
Ordered Locus Names:ELI_14285
OrganismiErythrobacter litoralis (strain HTCC2594)
Taxonomic identifieri314225 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesErythrobacteraceaeErythrobacter
Proteomesi
  • UP000008808 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003029111 – 403S-adenosylmethionine synthaseAdd BLAST403

Proteomic databases

PRIDEiQ2N5U2

Interactioni

Subunit structurei

Homotetramer; dimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi314225.ELI_14285

Structurei

3D structure databases

ProteinModelPortaliQ2N5U2
SMRiQ2N5U2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni110 – 120Flexible loopUniRule annotationAdd BLAST11

Sequence similaritiesi

Belongs to the AdoMet synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPH Bacteria
COG0192 LUCA
HOGENOMiHOG000245710
KOiK00789
OMAiPGHFLFT
OrthoDBiPOG091H00IO

Family and domain databases

HAMAPiMF_00086 S_AdoMet_synth1, 1 hit
InterProiView protein in InterPro
IPR022631 ADOMET_SYNTHASE_CS
IPR022630 S-AdoMet_synt_C
IPR022629 S-AdoMet_synt_central
IPR022628 S-AdoMet_synt_N
IPR002133 S-AdoMet_synthetase
IPR022636 S-AdoMet_synthetase_sfam
PANTHERiPTHR11964 PTHR11964, 1 hit
PfamiView protein in Pfam
PF02773 S-AdoMet_synt_C, 1 hit
PF02772 S-AdoMet_synt_M, 1 hit
PF00438 S-AdoMet_synt_N, 1 hit
PIRSFiPIRSF000497 MAT, 1 hit
SUPFAMiSSF55973 SSF55973, 3 hits
TIGRFAMsiTIGR01034 metK, 1 hit
PROSITEiView protein in PROSITE
PS00376 ADOMET_SYNTHASE_1, 1 hit
PS00377 ADOMET_SYNTHASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q2N5U2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRSTYLFTSE SVSEGHPDKV SDQISDAIVD LFIGKDPEAR VACETLTTTE
60 70 80 90 100
RVILAGEIRG QGIMDTDGNW APGIEDEIEA AVRQTVKEIG YEQDGFHWET
110 120 130 140 150
LTFENHLHGQ SAHIAQGVDA SGNKDEGAGD QGIMFGFACD ETPDLMPAPI
160 170 180 190 200
DYSHKILHRL ATDRKNGNAP FLEPDSKSQV TLRYENGKPV ACTAVVVSTQ
210 220 230 240 250
HGAGYDEGEK EAELRAYVKR VVADILPDGF LSDATKWHIN PTGTFVIGGP
260 270 280 290 300
DGDAGLTGRK IIVDTYGGAA PHGGGAFSGK DPTKVDRSAA YITRYLAKNV
310 320 330 340 350
VAAGLATRCT IQIAYAIGVS QPLSLYVDTH GTGTVDDAAI EEAILDIEKL
360 370 380 390 400
GGLTPRAIRT HLGLNKPIYR VSAAYGHFGR TAEGDRFPWE RTDLVEDLKA

ALA
Length:403
Mass (Da):43,297
Last modified:February 7, 2006 - v1
Checksum:i07199B0D47648AB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000157 Genomic DNA Translation: ABC64949.1
RefSeqiWP_011415771.1, NC_007722.1

Genome annotation databases

EnsemblBacteriaiABC64949; ABC64949; ELI_14285
KEGGieli:ELI_14285

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000157 Genomic DNA Translation: ABC64949.1
RefSeqiWP_011415771.1, NC_007722.1

3D structure databases

ProteinModelPortaliQ2N5U2
SMRiQ2N5U2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi314225.ELI_14285

Proteomic databases

PRIDEiQ2N5U2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC64949; ABC64949; ELI_14285
KEGGieli:ELI_14285

Phylogenomic databases

eggNOGiENOG4105CPH Bacteria
COG0192 LUCA
HOGENOMiHOG000245710
KOiK00789
OMAiPGHFLFT
OrthoDBiPOG091H00IO

Enzyme and pathway databases

UniPathwayi
UPA00315;UER00080

BioCyciELIT314225:G1G5F-2829-MONOMER

Family and domain databases

HAMAPiMF_00086 S_AdoMet_synth1, 1 hit
InterProiView protein in InterPro
IPR022631 ADOMET_SYNTHASE_CS
IPR022630 S-AdoMet_synt_C
IPR022629 S-AdoMet_synt_central
IPR022628 S-AdoMet_synt_N
IPR002133 S-AdoMet_synthetase
IPR022636 S-AdoMet_synthetase_sfam
PANTHERiPTHR11964 PTHR11964, 1 hit
PfamiView protein in Pfam
PF02773 S-AdoMet_synt_C, 1 hit
PF02772 S-AdoMet_synt_M, 1 hit
PF00438 S-AdoMet_synt_N, 1 hit
PIRSFiPIRSF000497 MAT, 1 hit
SUPFAMiSSF55973 SSF55973, 3 hits
TIGRFAMsiTIGR01034 metK, 1 hit
PROSITEiView protein in PROSITE
PS00376 ADOMET_SYNTHASE_1, 1 hit
PS00377 ADOMET_SYNTHASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMETK_ERYLH
AccessioniPrimary (citable) accession number: Q2N5U2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: February 7, 2006
Last modified: October 10, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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