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Protein

Hyaluronidase-4

Gene

HYAL4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endo-hyaluronidase that degrades hyaluronan to smaller oligosaccharide fragments. Has also chondroitin sulfate hydrolase activity, The best substrate being the galactosaminidic linkage in the sequence of a trisulfated tetrasaccharide.2 Publications

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

pH dependencei

Optimum pH is 4.5-5 (for chondroitin sulfate hydrolase activity).1 Publication

Temperature dependencei

Optimum temperature is 37 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei147Proton donorBy similarity1

GO - Molecular functioni

  • hyalurononglucosaminidase activity Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:HS02883-MONOMER
BRENDAi3.2.1.35 2681

Protein family/group databases

CAZyiGH56 Glycoside Hydrolase Family 56

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase-4 (EC:3.2.1.35)
Short name:
Hyal-4
Alternative name(s):
Chondroitin sulfate endo-beta-N-acetylgalactosaminidase
Chondroitin sulfate hydrolase
Short name:
CSHY
Hyaluronoglucosaminidase-4
Gene namesi
Name:HYAL4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000106302.9
HGNCiHGNC:5323 HYAL4
MIMi604510 gene
neXtProtiNX_Q2M3T9

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Topological domaini30 – 453ExtracellularSequence analysisAdd BLAST424
Transmembranei454 – 474HelicalSequence analysisAdd BLAST21
Topological domaini475 – 481CytoplasmicSequence analysis7

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi23553
OpenTargetsiENSG00000106302
PharmGKBiPA29574

Polymorphism and mutation databases

BioMutaiHYAL4
DMDMi158564281

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003019991 – 481Hyaluronidase-4Add BLAST481

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi59 ↔ 351By similarity
Glycosylationi86N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi115N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi177N-linked (GlcNAc...) (complex) asparagine1 Publication1
Disulfide bondi223 ↔ 237By similarity
Glycosylationi343N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi376 ↔ 387By similarity
Disulfide bondi381 ↔ 435By similarity
Disulfide bondi437 ↔ 446By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ2M3T9
PRIDEiQ2M3T9
ProteomicsDBi61382

PTM databases

iPTMnetiQ2M3T9
PhosphoSitePlusiQ2M3T9

Expressioni

Tissue specificityi

Detected in placenta and skeletal muscle.1 Publication

Gene expression databases

BgeeiENSG00000106302
CleanExiHS_HYAL4
ExpressionAtlasiQ2M3T9 baseline and differential
GenevisibleiQ2M3T9 HS

Organism-specific databases

HPAiHPA029453

Interactioni

Protein-protein interaction databases

BioGridi117097, 5 interactors
STRINGi9606.ENSP00000223026

Structurei

3D structure databases

ProteinModelPortaliQ2M3T9
SMRiQ2M3T9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Curated

Keywords - Domaini

EGF-like domain, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IECJ Eukaryota
ENOG410XPZT LUCA
GeneTreeiENSGT00550000074476
HOGENOMiHOG000015133
HOVERGENiHBG052053
InParanoidiQ2M3T9
KOiK01197
OMAiLYPSIGV
OrthoDBiEOG091G064G
PhylomeDBiQ2M3T9
TreeFamiTF321598

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR017853 Glycoside_hydrolase_SF
IPR018155 Hyaluronidase
PANTHERiPTHR11769 PTHR11769, 1 hit
PfamiView protein in Pfam
PF01630 Glyco_hydro_56, 1 hit
PIRSFiPIRSF038193 Hyaluronidase, 1 hit
PRINTSiPR00846 GLHYDRLASE56
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q2M3T9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLSEGQLK LCVVQPVHLT SWLLIFFILK SISCLKPARL PIYQRKPFIA
60 70 80 90 100
AWNAPTDQCL IKYNLRLNLK MFPVIGSPLA KARGQNVTIF YVNRLGYYPW
110 120 130 140 150
YTSQGVPING GLPQNISLQV HLEKADQDIN YYIPAEDFSG LAVIDWEYWR
160 170 180 190 200
PQWARNWNSK DVYRQKSRKL ISDMGKNVSA TDIEYLAKVT FEESAKAFMK
210 220 230 240 250
ETIKLGIKSR PKGLWGYYLY PDCHNYNVYA PNYSGSCPED EVLRNNELSW
260 270 280 290 300
LWNSSAALYP SIGVWKSLGD SENILRFSKF RVHESMRIST MTSHDYALPV
310 320 330 340 350
FVYTRLGYRD EPLFFLSKQD LVSTIGESAA LGAAGIVIWG DMNLTASKAN
360 370 380 390 400
CTKVKQFVSS DLGSYIANVT RAAEVCSLHL CRNNGRCIRK MWNAPSYLHL
410 420 430 440 450
NPASYHIEAS EDGEFTVKGK ASDTDLAVMA DTFSCHCYQG YEGADCREIK
460 470 480
TADGCSGVSP SPGSLMTLCL LLLASYRSIQ L
Length:481
Mass (Da):54,249
Last modified:September 11, 2007 - v2
Checksum:i9D530009AA898D1F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti263G → C in AAC98883 (PubMed:10493834).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_034936346A → S1 PublicationCorresponds to variant dbSNP:rs6949082Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF009010 mRNA Translation: AAC98883.1
AB470346 mRNA Translation: BAI49593.1
AC006029 Genomic DNA Translation: AAD43186.1
CH236947 Genomic DNA Translation: EAL24331.1
CH471070 Genomic DNA Translation: EAW83603.1
BC104788 mRNA Translation: AAI04789.1
BC104790 mRNA Translation: AAI04791.1
CCDSiCCDS5789.1
RefSeqiNP_036401.2, NM_012269.2
XP_011514292.1, XM_011515990.2
XP_016867400.1, XM_017011911.1
UniGeneiHs.28673

Genome annotation databases

EnsembliENST00000223026; ENSP00000223026; ENSG00000106302
ENST00000476325; ENSP00000417186; ENSG00000106302
GeneIDi23553
KEGGihsa:23553
UCSCiuc003vlc.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiHYAL4_HUMAN
AccessioniPrimary (citable) accession number: Q2M3T9
Secondary accession number(s): D0VXG1, Q9UL99, Q9Y6T9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: June 20, 2018
This is version 105 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

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