UniProtKB - Q2KIZ8 (MCM6_BOVIN)
Protein
DNA replication licensing factor MCM6
Gene
MCM6
Organism
Bos taurus (Bovine)
Status
Functioni
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.By similarity
Catalytic activityi
- EC:3.6.4.12
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 396 – 403 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA binding Source: UniProtKB-KW
- DNA helicase activity Source: UniProtKB-EC
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- DNA replication initiation Source: InterPro
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | Cell cycle, DNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:MCM6 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- Chromosome By similarity
Note: Binds to chromatin during G1 and detach from it during S phase.By similarity
Nucleus
- CMG complex Source: UniProtKB
Other locations
- MCM complex Source: UniProtKB
Keywords - Cellular componenti
Chromosome, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000239660 | 1 – 821 | DNA replication licensing factor MCM6Add BLAST | 821 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 13 | PhosphoserineBy similarity | 1 | |
Modified residuei | 219 | PhosphoserineBy similarity | 1 | |
Modified residuei | 271 | PhosphoserineBy similarity | 1 | |
Modified residuei | 278 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 643 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 689 | PhosphoserineBy similarity | 1 | |
Modified residuei | 762 | PhosphoserineBy similarity | 1 | |
Modified residuei | 791 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.By similarity
Keywords - PTMi
Acetylation, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | Q2KIZ8 |
PRIDEi | Q2KIZ8 |
Interactioni
Subunit structurei
Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. May interact with MCM10.
Interacts with TIPIN.
Interacts with CDT1.
Interacts with MCMBP.
Interacts with DDI2 (By similarity).
Component of the CMG helicase complex, composed of the MCM2-7 complex, the GINS complex and CDC45 (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000048625 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 346 – 553 | MCMAdd BLAST | 208 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 528 – 531 | Arginine finger | 4 |
Sequence similaritiesi
Belongs to the MCM family.Curated
Phylogenomic databases
eggNOGi | KOG0480, Eukaryota |
InParanoidi | Q2KIZ8 |
OMAi | KYYIAQI |
OrthoDBi | 266497at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR031327, MCM IPR008049, MCM6 IPR041024, Mcm6_C IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF18263, MCM6_C, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01662, MCMPROTEIN6 |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q2KIZ8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDLAAAAEPG AGSQHLEVRD EVAEKCQKLF LDFLEEFQNS DGEIKYLQLA
60 70 80 90 100
EELIRPERNT LVVSFVDLEQ FNQQLSTTIQ EEFYRVYPYL CRALKTFVKD
110 120 130 140 150
RKEIPLAKDF YVAFQDLPTR HKIRELTSSR IGLLTRISGQ VVRTHPVHPE
160 170 180 190 200
LVSGTFLCLD CQTVIKDVEQ QFKYTQPNIC RNPVCANRRR FLLDTNKSRF
210 220 230 240 250
VDFQKVRIQE TQAELPRGSI PRSLEVILRA EAVESAQAGD KCDFTGTLIV
260 270 280 290 300
VPDVSKLSTP GARAETDSRV SGVDGYETEG VRGLRALGVR DLSYRLVFLA
310 320 330 340 350
CCVAPTNPRF GGKELRDEEQ TAESIKNQMT VKEWEKVFEM SQDKNLYHNL
360 370 380 390 400
CTSLFPTIHG NDEVKRGVLL MLFGGVPKTT GEGTSLRGDI NVCIVGDPST
410 420 430 440 450
AKSQFLKHVE EFSPRAVYTS GKASIAAGLT AAVVRDEESH EFVIEAGALM
460 470 480 490 500
LADNGVCCID EFDKMDVRDQ VAIHEAMEQQ TISITKAGVK ATLNARTSIL
510 520 530 540 550
AAANPISGHY DRSKSLKQNI NLSAPIMSRF DLFFILVDEC NEVTDYAIAR
560 570 580 590 600
RIVDLHSRIE DSIDRVYSLD EIRRYLLFAR QFKPKISKES EDFIVEQYKR
610 620 630 640 650
LRQRDGSGVT KSSWRITVRQ LESMIRLSEA MARMHCCDEV QPKHVKEAFR
660 670 680 690 700
LLNKSIIRVE TPDVNLDQEE DAQMEVDEGP DGINGHADSP APASGINGHS
710 720 730 740 750
EDMNQDSVPK ASLRLGFSEY CRISNLIVLH LRKMEEEEDE SALKRSELVN
760 770 780 790 800
WYLKEIESEI DSEEELINKK RIIEKVIYRL THYDHVLIEL TQAGLKGSTE
810 820
GSESYEEDPY LVVNPNYLLE D
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC112448 mRNA Translation: AAI12449.1 |
RefSeqi | NP_001039699.1, NM_001046234.1 |
Genome annotation databases
GeneIDi | 517812 |
KEGGi | bta:517812 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC112448 mRNA Translation: AAI12449.1 |
RefSeqi | NP_001039699.1, NM_001046234.1 |
3D structure databases
BMRBi | Q2KIZ8 |
SMRi | Q2KIZ8 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000048625 |
Proteomic databases
PaxDbi | Q2KIZ8 |
PRIDEi | Q2KIZ8 |
Genome annotation databases
GeneIDi | 517812 |
KEGGi | bta:517812 |
Organism-specific databases
CTDi | 4175 |
Phylogenomic databases
eggNOGi | KOG0480, Eukaryota |
InParanoidi | Q2KIZ8 |
OMAi | KYYIAQI |
OrthoDBi | 266497at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR031327, MCM IPR008049, MCM6 IPR041024, Mcm6_C IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF18263, MCM6_C, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01662, MCMPROTEIN6 |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCM6_BOVIN | |
Accessioni | Q2KIZ8Primary (citable) accession number: Q2KIZ8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 13, 2006 |
Last sequence update: | March 7, 2006 | |
Last modified: | April 7, 2021 | |
This is version 92 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families