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Protein

Histidine--tRNA ligase, cytoplasmic

Gene

HARS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Cytoplasmic histidine--tRNA ligase. Plays a role in axon guidance.By similarity

Catalytic activityi

ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei157L-histidineBy similarity1
Binding sitei173L-histidineBy similarity1
Binding sitei177L-histidineBy similarity1
Binding sitei326L-histidineBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine--tRNA ligase, cytoplasmic (EC:6.1.1.21)
Alternative name(s):
Histidyl-tRNA synthetase
Short name:
HisRS
Gene namesi
Name:HARS
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 7

Organism-specific databases

VGNCiVGNC:29753 HARS

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002450212 – 509Histidine--tRNA ligase, cytoplasmicAdd BLAST508

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei66PhosphoserineBy similarity1
Modified residuei356PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ2KI84
PeptideAtlasiQ2KI84
PRIDEiQ2KI84

Expressioni

Gene expression databases

BgeeiENSBTAG00000018847 Expressed in 10 organ(s), highest expression level in brain

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000025094

Structurei

3D structure databases

ProteinModelPortaliQ2KI84
SMRiQ2KI84
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 59WHEP-TRSAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni130 – 132L-histidine bindingBy similarity3
Regioni330 – 331L-histidine bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1936 Eukaryota
COG0124 LUCA
GeneTreeiENSGT00390000005922
HOGENOMiHOG000018075
HOVERGENiHBG002731
InParanoidiQ2KI84
KOiK01892
OMAiCDFDFIG
OrthoDBiEOG091G05P3
TreeFamiTF300652

Family and domain databases

CDDicd00859 HisRS_anticodon, 1 hit
Gene3Di3.40.50.800, 1 hit
HAMAPiMF_00127 His_tRNA_synth, 1 hit
InterProiView protein in InterPro
IPR006195 aa-tRNA-synth_II
IPR004154 Anticodon-bd
IPR036621 Anticodon-bd_dom_sf
IPR015807 His-tRNA-ligase
IPR004516 HisRS/HisZ
IPR033656 HisRS_anticodon
IPR009068 S15_NS1_RNA-bd
IPR000738 WHEP-TRS_dom
PANTHERiPTHR11476 PTHR11476, 1 hit
PfamiView protein in Pfam
PF03129 HGTP_anticodon, 1 hit
PF00458 WHEP-TRS, 1 hit
PIRSFiPIRSF001549 His-tRNA_synth, 1 hit
SMARTiView protein in SMART
SM00991 WHEP-TRS, 1 hit
SUPFAMiSSF47060 SSF47060, 1 hit
TIGRFAMsiTIGR00442 hisS, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit
PS00762 WHEP_TRS_1, 1 hit
PS51185 WHEP_TRS_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2KI84-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADRAALEDL VRVQGERVRG LKQQKASAEQ IEEEVAKLLK LKAQLGPDEG
60 70 80 90 100
KPKFVLKTPK GTRDYSPRQM AVREKVFDVI ISCFKRHGAE VIDTPVFELK
110 120 130 140 150
ETLTGKYGED SKLIYDLKDQ GGELLSLRYD LTVPFARYLA MNKLTNIKRY
160 170 180 190 200
HIAKVYRRDN PAMTRGRYRE FYQCDFDIAG QFDPMLPDAE CLKIMCEILS
210 220 230 240 250
SLQIGDFLVK VNDRRILDGM FAICGVPDSK FRTICSSVDK LDKVSWEEVK
260 270 280 290 300
NEMVGEKGLA PEVADRIGDY VQQHGGVSLV EQLLQDPKLS QNKQALEGLG
310 320 330 340 350
DLKLLFEYLT LFGIADKISF DLSLARGLDY YTGVIYEAVL LQPPARAGEE
360 370 380 390 400
PLGVGSVAAG GRYDGLVGMF DPKGRKVPCV GLSIGVERIF SIVEQRLEAL
410 420 430 440 450
EEKVRTTETQ VLVASAQKKL LEERLKLISE LWDAGIKAEL LYKKNPKLLN
460 470 480 490 500
QLQYCEETGI PLVAIIGEQE LKDGVIKLRS VASREEVDVR REDLVEEIKR

RTSQPLCIC
Length:509
Mass (Da):57,285
Last modified:March 7, 2006 - v1
Checksum:i54A2CAFD638D4358
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112731 mRNA Translation: AAI12732.1
RefSeqiNP_001039531.1, NM_001046066.2
UniGeneiBt.49179

Genome annotation databases

EnsembliENSBTAT00000025094; ENSBTAP00000025094; ENSBTAG00000018847
GeneIDi510937
KEGGibta:510937

Similar proteinsi

Entry informationi

Entry nameiSYHC_BOVIN
AccessioniPrimary (citable) accession number: Q2KI84
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: March 7, 2006
Last modified: September 12, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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