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UniProtKB - Q2KI84 (HARS1_BOVIN)
Protein
Histidine--tRNA ligase, cytoplasmic
Gene
HARS1
Organism
Bos taurus (Bovine)
Status
Functioni
Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP). Plays a role in axon guidance.
By similarityCatalytic activityi
- EC:6.1.1.21By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 157 | L-histidineBy similarity | 1 | |
Binding sitei | 173 | L-histidineBy similarity | 1 | |
Binding sitei | 177 | L-histidineBy similarity | 1 | |
Binding sitei | 326 | L-histidineBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- histidine-tRNA ligase activity Source: UniProtKB
- identical protein binding Source: GO_Central
- protein homodimerization activity Source: UniProtKB
GO - Biological processi
- histidyl-tRNA aminoacylation Source: UniProtKB
- mitochondrial translation Source: GO_Central
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Histidine--tRNA ligase, cytoplasmic (EC:6.1.1.21By similarity)Alternative name(s): Histidyl-tRNA synthetase Short name: HisRS |
Gene namesi | Name:HARS1 Synonyms:HARS |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000018847 |
VGNCi | VGNC:29753, HARS1 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytosol
- cytosol Source: GO_Central
Mitochondrion
- mitochondrion Source: GO_Central
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000245021 | 2 – 509 | Histidine--tRNA ligase, cytoplasmicAdd BLAST | 508 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 66 | PhosphoserineBy similarity | 1 | |
Modified residuei | 356 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | Q2KI84 |
PeptideAtlasi | Q2KI84 |
PRIDEi | Q2KI84 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000018847, Expressed in vertebral column and 98 other tissues |
Interactioni
Subunit structurei
Homodimer.
By similarityGO - Molecular functioni
- identical protein binding Source: GO_Central
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000025094 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 59 | WHEP-TRSAdd BLAST | 57 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 130 – 132 | L-histidine bindingBy similarity | 3 | |
Regioni | 330 – 331 | L-histidine bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the class-II aminoacyl-tRNA synthetase family.Curated
Phylogenomic databases
eggNOGi | KOG1936, Eukaryota |
GeneTreei | ENSGT00390000005922 |
HOGENOMi | CLU_025113_4_2_1 |
InParanoidi | Q2KI84 |
OMAi | CDFDFIG |
OrthoDBi | 1065556at2759 |
TreeFami | TF300652 |
Family and domain databases
CDDi | cd00773, HisRS-like_core, 1 hit cd00859, HisRS_anticodon, 1 hit |
Gene3Di | 3.30.930.10, 1 hit 3.40.50.800, 1 hit |
HAMAPi | MF_00127, His_tRNA_synth, 1 hit |
InterProi | View protein in InterPro IPR006195, aa-tRNA-synth_II IPR045864, aa-tRNA-synth_II/BPL/LPL IPR004154, Anticodon-bd IPR036621, Anticodon-bd_dom_sf IPR015807, His-tRNA-ligase IPR041715, HisRS-like_core IPR004516, HisRS/HisZ IPR033656, HisRS_anticodon IPR009068, S15_NS1_RNA-bd IPR000738, WHEP-TRS_dom |
Pfami | View protein in Pfam PF03129, HGTP_anticodon, 1 hit PF13393, tRNA-synt_His, 1 hit PF00458, WHEP-TRS, 1 hit |
PIRSFi | PIRSF001549, His-tRNA_synth, 1 hit |
SMARTi | View protein in SMART SM00991, WHEP-TRS, 1 hit |
SUPFAMi | SSF47060, SSF47060, 1 hit SSF55681, SSF55681, 1 hit |
TIGRFAMsi | TIGR00442, hisS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit PS00762, WHEP_TRS_1, 1 hit PS51185, WHEP_TRS_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q2KI84-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADRAALEDL VRVQGERVRG LKQQKASAEQ IEEEVAKLLK LKAQLGPDEG
60 70 80 90 100
KPKFVLKTPK GTRDYSPRQM AVREKVFDVI ISCFKRHGAE VIDTPVFELK
110 120 130 140 150
ETLTGKYGED SKLIYDLKDQ GGELLSLRYD LTVPFARYLA MNKLTNIKRY
160 170 180 190 200
HIAKVYRRDN PAMTRGRYRE FYQCDFDIAG QFDPMLPDAE CLKIMCEILS
210 220 230 240 250
SLQIGDFLVK VNDRRILDGM FAICGVPDSK FRTICSSVDK LDKVSWEEVK
260 270 280 290 300
NEMVGEKGLA PEVADRIGDY VQQHGGVSLV EQLLQDPKLS QNKQALEGLG
310 320 330 340 350
DLKLLFEYLT LFGIADKISF DLSLARGLDY YTGVIYEAVL LQPPARAGEE
360 370 380 390 400
PLGVGSVAAG GRYDGLVGMF DPKGRKVPCV GLSIGVERIF SIVEQRLEAL
410 420 430 440 450
EEKVRTTETQ VLVASAQKKL LEERLKLISE LWDAGIKAEL LYKKNPKLLN
460 470 480 490 500
QLQYCEETGI PLVAIIGEQE LKDGVIKLRS VASREEVDVR REDLVEEIKR
RTSQPLCIC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC112731 mRNA Translation: AAI12732.1 |
RefSeqi | NP_001039531.1, NM_001046066.2 |
Genome annotation databases
Ensembli | ENSBTAT00000025094; ENSBTAP00000025094; ENSBTAG00000018847 |
GeneIDi | 510937 |
KEGGi | bta:510937 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC112731 mRNA Translation: AAI12732.1 |
RefSeqi | NP_001039531.1, NM_001046066.2 |
3D structure databases
AlphaFoldDBi | Q2KI84 |
SMRi | Q2KI84 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000025094 |
Proteomic databases
PaxDbi | Q2KI84 |
PeptideAtlasi | Q2KI84 |
PRIDEi | Q2KI84 |
Genome annotation databases
Ensembli | ENSBTAT00000025094; ENSBTAP00000025094; ENSBTAG00000018847 |
GeneIDi | 510937 |
KEGGi | bta:510937 |
Organism-specific databases
CTDi | 3035 |
VEuPathDBi | HostDB:ENSBTAG00000018847 |
VGNCi | VGNC:29753, HARS1 |
Phylogenomic databases
eggNOGi | KOG1936, Eukaryota |
GeneTreei | ENSGT00390000005922 |
HOGENOMi | CLU_025113_4_2_1 |
InParanoidi | Q2KI84 |
OMAi | CDFDFIG |
OrthoDBi | 1065556at2759 |
TreeFami | TF300652 |
Gene expression databases
Bgeei | ENSBTAG00000018847, Expressed in vertebral column and 98 other tissues |
Family and domain databases
CDDi | cd00773, HisRS-like_core, 1 hit cd00859, HisRS_anticodon, 1 hit |
Gene3Di | 3.30.930.10, 1 hit 3.40.50.800, 1 hit |
HAMAPi | MF_00127, His_tRNA_synth, 1 hit |
InterProi | View protein in InterPro IPR006195, aa-tRNA-synth_II IPR045864, aa-tRNA-synth_II/BPL/LPL IPR004154, Anticodon-bd IPR036621, Anticodon-bd_dom_sf IPR015807, His-tRNA-ligase IPR041715, HisRS-like_core IPR004516, HisRS/HisZ IPR033656, HisRS_anticodon IPR009068, S15_NS1_RNA-bd IPR000738, WHEP-TRS_dom |
Pfami | View protein in Pfam PF03129, HGTP_anticodon, 1 hit PF13393, tRNA-synt_His, 1 hit PF00458, WHEP-TRS, 1 hit |
PIRSFi | PIRSF001549, His-tRNA_synth, 1 hit |
SMARTi | View protein in SMART SM00991, WHEP-TRS, 1 hit |
SUPFAMi | SSF47060, SSF47060, 1 hit SSF55681, SSF55681, 1 hit |
TIGRFAMsi | TIGR00442, hisS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit PS00762, WHEP_TRS_1, 1 hit PS51185, WHEP_TRS_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HARS1_BOVIN | |
Accessioni | Q2KI84Primary (citable) accession number: Q2KI84 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2006 |
Last sequence update: | March 7, 2006 | |
Last modified: | May 25, 2022 | |
This is version 110 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - SIMILARITY comments
Index of protein domains and families