HARS

Functionⁱ

Cytoplasmic histidine--tRNA ligase. Plays a role in axon guidance.By similarity

Catalytic activityⁱ

ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	157	L-histidineBy similarity	1
Binding siteⁱ	173	L-histidineBy similarity	1
Binding siteⁱ	177	L-histidineBy similarity	1
Binding siteⁱ	326	L-histidineBy similarity	1

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
histidine-tRNA ligase activity Source: UniProtKB-EC
identical protein binding Source: Ensembl

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

histidyl-tRNA aminoacylation Source: InterPro
mitochondrial translation Source: GO_Central

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Aminoacyl-tRNA synthetase, Ligase
Biological process	Protein biosynthesis
Ligand	ATP-binding, Nucleotide-binding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Histidine--tRNA ligase, cytoplasmic (EC:6.1.1.21) Alternative name(s): Histidyl-tRNA synthetase Short name: HisRS
Gene namesⁱ	Name:HARS
Organismⁱ	Bos taurus (Bovine)
Taxonomic identifierⁱ	9913 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos
Proteomesⁱ	UP000009136 Componentⁱ: Chromosome 7

Organism-specific databases

VGNCⁱ	VGNC:29753 HARS

VGNCⁱ

VGNC:29753 HARS

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedBy similarity
ChainⁱPRO_0000245021	2 – 509	Histidine--tRNA ligase, cytoplasmicAdd BLAST		508

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	2	N-acetylalanineBy similarity	1
Modified residueⁱ	66	PhosphoserineBy similarity	1
Modified residueⁱ	356	PhosphoserineBy similarity	1

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

PaxDbⁱ	Q2KI84
PeptideAtlas More...PeptideAtlasⁱ	Q2KI84
PRIDEⁱ	Q2KI84

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSBTAG00000018847 Expressed in 10 organ(s), highest expression level in brain

Bgeeⁱ

ENSBTAG00000018847 Expressed in 10 organ(s), highest expression level in brain

Interactionⁱ

Subunit structureⁱ

Homodimer.By similarity

GO - Molecular functionⁱ

identical protein binding Source: Ensembl

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

STRINGⁱ	9913.ENSBTAP00000025094

STRINGⁱ

9913.ENSBTAP00000025094

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q2KI84
SMRⁱ	Q2KI84
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	3 – 59	WHEP-TRSAdd BLAST		57

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	130 – 132	L-histidine bindingBy similarity		3
Regionⁱ	330 – 331	L-histidine bindingBy similarity		2

Sequence similaritiesⁱ

Belongs to the class-II aminoacyl-tRNA synthetase family.Curated

Phylogenomic databases

eggNOGⁱ	KOG1936 Eukaryota COG0124 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000005922
HOGENOMⁱ	HOG000018075
HOVERGENⁱ	HBG002731
InParanoidⁱ	Q2KI84
KEGG Orthology (KO) More...KOⁱ	K01892
OMAⁱ	CDFDFIG
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G05P3
TreeFamⁱ	TF300652

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00859 HisRS_anticodon, 1 hit
Gene3Dⁱ	3.40.50.800, 1 hit
HAMAPⁱ	MF_00127 His_tRNA_synth, 1 hit
InterProⁱ	View protein in InterPro IPR006195 aa-tRNA-synth_II IPR004154 Anticodon-bd IPR036621 Anticodon-bd_dom_sf IPR015807 His-tRNA-ligase IPR004516 HisRS/HisZ IPR033656 HisRS_anticodon IPR009068 S15_NS1_RNA-bd IPR000738 WHEP-TRS_dom
PANTHERⁱ	PTHR11476 PTHR11476, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF03129 HGTP_anticodon, 1 hit PF00458 WHEP-TRS, 1 hit
PIRSFⁱ	PIRSF001549 His-tRNA_synth, 1 hit
SMARTⁱ	View protein in SMART SM00991 WHEP-TRS, 1 hit
SUPFAMⁱ	SSF47060 SSF47060, 1 hit
TIGRFAMsⁱ	TIGR00442 hisS, 1 hit
PROSITEⁱ	View protein in PROSITE PS50862 AA_TRNA_LIGASE_II, 1 hit PS00762 WHEP_TRS_1, 1 hit PS51185 WHEP_TRS_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q2KI84-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MADRAALEDL VRVQGERVRG LKQQKASAEQ IEEEVAKLLK LKAQLGPDEG 
        60         70         80         90        100
KPKFVLKTPK GTRDYSPRQM AVREKVFDVI ISCFKRHGAE VIDTPVFELK 
       110        120        130        140        150
ETLTGKYGED SKLIYDLKDQ GGELLSLRYD LTVPFARYLA MNKLTNIKRY 
       160        170        180        190        200
HIAKVYRRDN PAMTRGRYRE FYQCDFDIAG QFDPMLPDAE CLKIMCEILS 
       210        220        230        240        250
SLQIGDFLVK VNDRRILDGM FAICGVPDSK FRTICSSVDK LDKVSWEEVK 
       260        270        280        290        300
NEMVGEKGLA PEVADRIGDY VQQHGGVSLV EQLLQDPKLS QNKQALEGLG 
       310        320        330        340        350
DLKLLFEYLT LFGIADKISF DLSLARGLDY YTGVIYEAVL LQPPARAGEE 
       360        370        380        390        400
PLGVGSVAAG GRYDGLVGMF DPKGRKVPCV GLSIGVERIF SIVEQRLEAL 
       410        420        430        440        450
EEKVRTTETQ VLVASAQKKL LEERLKLISE LWDAGIKAEL LYKKNPKLLN 
       460        470        480        490        500
QLQYCEETGI PLVAIIGEQE LKDGVIKLRS VASREEVDVR REDLVEEIKR 

RTSQPLCIC

Length:509

Mass (Da):57,285

Last modified:March 7, 2006 - v1

Checksum:ⁱ54A2CAFD638D4358

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BC112731 mRNA Translation: AAI12732.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001039531.1, NM_001046066.2
UniGeneⁱ	Bt.49179

Genome annotation databases

Ensemblⁱ	ENSBTAT00000025094; ENSBTAP00000025094; ENSBTAG00000018847
GeneIDⁱ	510937
KEGGⁱ	bta:510937

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q2KI84	Histidyl-tRNA synthetase, cytoplasmic (Fragment)		wild yak		512	UniRef90_Q2KI84
HARS		CEREH		509
Histidyl-tRNA synthetase		SHEEP		509
HARS protein (Fragment)		BOVIN		335
UPI000950F010		BOSIN		569
+9

Protein	Similar proteins		Species	Score	Length	Source
Q2KI84	Histidine--tRNA ligase, cytoplasmic	)	HUMAN		509	UniRef50_P12081
Histidine--tRNA ligase, cytoplasmic		MOUSE		509
Histidine--tRNA ligase, cytoplasmic		PONAB		509
Probable histidine--tRNA ligase, mitochondrial		BOVIN		506
Histidine--tRNA ligase, cytoplasmic (Fragment)		ESCRO		567
+258

Entry informationⁱ

Entry nameⁱ	SYHC_BOVIN
Accessionⁱ	Q2KI84Primary (citable) accession number: Q2KI84
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
	Last sequence update:	March 7, 2006
	Last modified:	September 12, 2018
	This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q2KI84 (SYHC_BOVIN)

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Histidine--tRNA ligase, cytoplasmic

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Cytosol

Mitochondrion

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ