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Protein

mRNA-capping enzyme

Gene

RNGTT

Organism
Bos taurus (Bovine)
Status
Unreviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.UniRule annotation

Catalytic activityi

A 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate.UniRule annotation
GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei126For RNA 5'-triphosphatase activityUniRule annotation1
Active sitei294N6-GMP-lysine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotation, NucleotidyltransferaseUniRule annotationImported, Transferase
Biological processmRNA cappingUniRule annotation, mRNA processing
LigandGTP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-72086 mRNA Capping
R-BTA-77075 RNA Pol II CTD phosphorylation and interaction with CE

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA-capping enzymeUniRule annotation
Including the following 2 domains:
Polynucleotide 5'-triphosphataseUniRule annotation (EC:3.1.3.33UniRule annotation)
mRNA guanylyltransferaseUniRule annotation (EC:2.7.7.50UniRule annotation)
Alternative name(s):
GTP--RNA guanylyltransferaseUniRule annotation
Short name:
GTaseUniRule annotation
Gene namesi
Name:RNGTTImported
OrganismiBos taurus (Bovine)Imported
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 9

Organism-specific databases

VGNCiVGNC:34076 RNGTT

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

NucleusUniRule annotation

Expressioni

Gene expression databases

BgeeiENSBTAG00000002725

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000003532

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 171TYR_PHOSPHATASE_2InterPro annotationAdd BLAST68

Sequence similaritiesi

In the C-terminal section; belongs to the eukaryotic GTase family.UniRule annotation
In the N-terminal section; belongs to the non-receptor class of the protein-tyrosine phosphatase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2386 Eukaryota
COG5226 LUCA
GeneTreeiENSGT00530000063473
HOGENOMiHOG000264524
HOVERGENiHBG006332
KOiK13917
OMAiKIIECTF
OrthoDBiEOG091G063D
TreeFamiTF314914

Family and domain databases

Gene3Di3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR017074 mRNA_cap_enz_bifunc
IPR001339 mRNA_cap_enzyme
IPR013846 mRNA_cap_enzyme_C
IPR012340 NA-bd_OB-fold
IPR029021 Prot-tyrosine_phosphatase-like
IPR016130 Tyr_Pase_AS
IPR000387 TYR_PHOSPHATASE_dom
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
PF03919 mRNA_cap_C, 1 hit
PF01331 mRNA_cap_enzyme, 1 hit
PIRSFiPIRSF036958 mRNA_capping_HCE, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q2KHX7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHNKIPPRW LNCPRRGQPV AGRFLPLKTM LGPRYDSQVA EENRFHPSML
60 70 80 90 100
SNYLKSLKVK MGLLVDLTNT SRFYDRNDIE KEGIKYIKLQ CKGHGECPTT
110 120 130 140 150
ENTETFIRLC ERFNERNPPE LIGVHCTHGF NRTGFLICAF LVEKMDWSIE
160 170 180 190 200
AAVATFAQAR PPGIYKGDYL KELFRRYGDI EEAPPPPLLP DWCFEDDEDE
210 220 230 240 250
DEDEDGKKES EPGSSASFGK RRKERLKLGA IFLEGVTVKG VTQVTTQPKL
260 270 280 290 300
GEVQQKCHQF CGWEGSGFPG AQPVSMDKQN IKLLEQKPYK VSWKADGTRY
310 320 330 340 350
MMLIDGTNEV FMIDRDNSVF HVSNLEFPFR KDLRMHLSNT LLDGEMIIDR
360 370 380 390 400
VNGQAVPRYL IYDIIKFNAQ PVGDCDFNIR LQCIEREIIN PRHEKMKTGL
410 420 430 440 450
IDKTQEPFSV RHKPFFDIYA SRKLLEGNFA KEVSHEMDGL IFQPTGKYKP
460 470 480 490 500
GRCDDILKWK PPSLNSVDFR LKITRMGGEG LLPQNIGLLY VGGYERPFAQ
510 520 530 540 550
IKVTKELKQY DNKIIECKFE NNSWVFMRQR TDKSFPNAYN TAMAVCNSIS
560 570 580 590
NPVTKEMLFE FIDRCAAASQ GQKRKHHPDP DTELMPPPPP KRPLT
Length:595
Mass (Da):68,378
Last modified:March 7, 2006 - v1
Checksum:iDE499DA841C749A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DAAA02026454 Genomic DNA No translation available.
DAAA02026455 Genomic DNA No translation available.
BC112845 mRNA Translation: AAI12846.1
RefSeqiNP_001039550.1, NM_001046085.2
UniGeneiBt.28442

Genome annotation databases

EnsembliENSBTAT00000003532; ENSBTAP00000003532; ENSBTAG00000002725
GeneIDi511408
KEGGibta:511408

Similar proteinsi

Entry informationi

Entry nameiQ2KHX7_BOVIN
AccessioniPrimary (citable) accession number: Q2KHX7
Entry historyiIntegrated into UniProtKB/TrEMBL: March 7, 2006
Last sequence update: March 7, 2006
Last modified: March 28, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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