UniProtKB - Q2KHP8 (TYW1_XENLA)
Protein
S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1
Gene
tyw1
Organism
Xenopus laevis (African clawed frog)
Status
Functioni
Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine, an intermediate in wybutosine biosynthesis (By similarity).By similarity
Catalytic activityi
- N1-methylguanosine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine37 in tRNAPhe + 5'-deoxyadenosine + CO2 + H2O + L-methionineEC:4.1.3.44
Cofactori
[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity
: wybutosine-tRNA(Phe) biosynthesis Pathwayi
This protein is involved in the pathway wybutosine-tRNA(Phe) biosynthesis, which is part of tRNA modification.View all proteins of this organism that are known to be involved in the pathway wybutosine-tRNA(Phe) biosynthesis and in tRNA modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 419 | Iron-sulfur (4Fe-4S-S-AdoMet)Sequence analysis | 1 | |
Metal bindingi | 423 | Iron-sulfur (4Fe-4S-S-AdoMet)Sequence analysis | 1 | |
Metal bindingi | 426 | Iron-sulfur (4Fe-4S-S-AdoMet)Sequence analysis | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 89 – 93 | FMNPROSITE-ProRule annotation | 5 | |
Nucleotide bindingi | 180 – 212 | FMNPROSITE-ProRule annotationAdd BLAST | 33 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- FMN binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
- tRNA-4-demethylwyosine synthase activity Source: UniProtKB-EC
GO - Biological processi
- oxidation-reduction process Source: InterPro
- tRNA processing Source: UniProtKB-KW
Keywordsi
Molecular function | Lyase |
Biological process | tRNA processing |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, S-adenosyl-L-methionine |
Enzyme and pathway databases
UniPathwayi | UPA00375 |
Names & Taxonomyi
Protein namesi | Recommended name: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 (EC:4.1.3.44)Alternative name(s): Radical S-adenosyl methionine and flavodoxin domain-containing protein 1 tRNA wybutosine-synthesizing protein 1 homolog tRNA-yW-synthesizing protein |
Gene namesi | Name:tyw1 Synonyms:rsafd1 |
Organismi | Xenopus laevis (African clawed frog) |
Taxonomic identifieri | 8355 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus |
Organism-specific databases
Xenbasei | XB-GENE-5955864, tyw1.S |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000281831 | 1 – 735 | S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1Add BLAST | 735 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 83 – 241 | Flavodoxin-likePROSITE-ProRule annotationAdd BLAST | 159 | |
Domaini | 403 – 649 | Radical SAM corePROSITE-ProRule annotationAdd BLAST | 247 |
Sequence similaritiesi
Belongs to the TYW1 family.Curated
Phylogenomic databases
OrthoDBi | 731783at2759 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit 3.40.50.360, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR029039, Flavoprotein-like_sf IPR007197, rSAM IPR013917, tRNA_wybutosine-synth IPR034556, tRNA_wybutosine-synthase |
Pfami | View protein in Pfam PF00258, Flavodoxin_1, 1 hit PF04055, Radical_SAM, 1 hit PF08608, Wyosine_form, 1 hit |
PRINTSi | PR00369, FLAVODOXIN |
SFLDi | SFLDF00284, tRNA_wybutosine-synthesizing, 1 hit |
SUPFAMi | SSF52218, SSF52218, 1 hit |
PROSITEi | View protein in PROSITE PS50902, FLAVODOXIN_LIKE, 1 hit PS51918, RADICAL_SAM, 1 hit |
i Sequence
Sequence statusi: Complete.
Q2KHP8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDKSTDAWDF AYTHLMFLWL NRFYIYSCAA IGITVWVCSH FIMARKKHQN
60 70 80 90 100
ETVCNGSLLL KTKALKSDSL TSNKEQKVYV AGVKIFYGSQ TGTSKGFAHL
110 120 130 140 150
LAEEVTLLGL PVELINMKEY DPDDNLVEET TSKNICVFLV ATYTDGKPPE
160 170 180 190 200
SAEWFCKWLE EASNDFRFGK TYLKGMRYAV FGLGNSVYST HYNTVGKNID
210 220 230 240 250
KWLWMLSANR VMTRAEGDCN VVKSKHGSIE SDFEAWKRKF LNRLKALLSG
260 270 280 290 300
EKKPCSGKCK KGKCKSKKKS SIESVEEEEE EEEKHSEHED TEDDTFETSS
310 320 330 340 350
DSEPEEHGEP GSGLIDVEDL GKAMSNMKKS KREHDSNTEL GKSLQGDEAK
360 370 380 390 400
EKEEPREMIT PALQEALTKQ GYRLIGSHSG VKLCRWTKSM LRGRGGCYKH
410 420 430 440 450
TFYGIESHRC METTPSLACA NKCVFCWRHH TNPVGTEWRW KMDQPEMILE
460 470 480 490 500
EAILNHQNMI KQFKGVPGVK PERFEEGLAV KHCALSLVGE PIMYPEINTF
510 520 530 540 550
LRLLHNQHIS SFLVTNAQFP EEIRSLEPVT QLYVSVDAST KDSLKKIDRP
560 570 580 590 600
LFKDFWQRFI DSLKALAEKQ QRTVYRLTLV KAWNVDELKA YADLVALGLP
610 620 630 640 650
DFIEVKGVTY CGESSASNLT MANVPWHEEV IRFVEELVDL LPDYEVACEH
660 670 680 690 700
EHSNCMLIAH KKFKINGEWC TWIDYERFQE LIQAFGESKG SRTFTALDYV
710 720 730
AKTPEWALFG SRERGFDPLD KRFQRKNKTK DISGC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC112954 mRNA Translation: AAI12955.1 |
RefSeqi | NP_001089965.1, NM_001096496.1 |
Genome annotation databases
GeneIDi | 735035 |
KEGGi | xla:735035 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC112954 mRNA Translation: AAI12955.1 |
RefSeqi | NP_001089965.1, NM_001096496.1 |
3D structure databases
SMRi | Q2KHP8 |
ModBasei | Search... |
Genome annotation databases
GeneIDi | 735035 |
KEGGi | xla:735035 |
Organism-specific databases
CTDi | 735035 |
Xenbasei | XB-GENE-5955864, tyw1.S |
Phylogenomic databases
OrthoDBi | 731783at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00375 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit 3.40.50.360, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR029039, Flavoprotein-like_sf IPR007197, rSAM IPR013917, tRNA_wybutosine-synth IPR034556, tRNA_wybutosine-synthase |
Pfami | View protein in Pfam PF00258, Flavodoxin_1, 1 hit PF04055, Radical_SAM, 1 hit PF08608, Wyosine_form, 1 hit |
PRINTSi | PR00369, FLAVODOXIN |
SFLDi | SFLDF00284, tRNA_wybutosine-synthesizing, 1 hit |
SUPFAMi | SSF52218, SSF52218, 1 hit |
PROSITEi | View protein in PROSITE PS50902, FLAVODOXIN_LIKE, 1 hit PS51918, RADICAL_SAM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TYW1_XENLA | |
Accessioni | Q2KHP8Primary (citable) accession number: Q2KHP8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2007 |
Last sequence update: | March 7, 2006 | |
Last modified: | December 2, 2020 | |
This is version 64 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families