UniProtKB - Q2I8V6 (AFR_ENSAD)
Protein
1,5-anhydro-D-fructose reductase
Gene
afr
Organism
Ensifer adhaerens (Sinorhizobium morelense)
Status
Functioni
Catalyzes the NADPH-specific reduction of 1,5-anhydro-D-fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro-D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as cosubstrate.
Catalytic activityi
- EC:1.1.1.2921 Publication
Kineticsi
- KM=8.4 mM for 1,5-anhydro-D-fructose1 Publication
- KM=11 mM for D-glucosone1 Publication
- KM=0.2 mM for NADPH1 Publication
- Vmax=500 µmol/min/mg enzyme1 Publication
pH dependencei
Optimum pH is 6.5 at 30 degrees Celsius.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 38 | NADP1 Publication | 1 | |
Binding sitei | 120 | NADP1 Publication | 1 | |
Binding sitei | 283 | NADP1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 9 – 12 | NADP1 Publication | 4 | |
Nucleotide bindingi | 33 – 34 | NADP1 Publication | 2 | |
Nucleotide bindingi | 71 – 76 | NADP1 Publication | 6 | |
Nucleotide bindingi | 93 – 94 | NADP1 Publication | 2 | |
Nucleotide bindingi | 162 – 163 | NADP1 Publication | 2 |
GO - Molecular functioni
- 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) activity Source: UniProtKB-EC
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NADP |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-16296 |
BRENDAi | 1.1.1.292, 8538 |
SABIO-RKi | Q2I8V6 |
Names & Taxonomyi
Protein namesi | Recommended name: 1,5-anhydro-D-fructose reductase (EC:1.1.1.292)Short name: Anhydrofructose reductase Alternative name(s): 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) |
Gene namesi | Name:afr |
Organismi | Ensifer adhaerens (Sinorhizobium morelense) |
Taxonomic identifieri | 106592 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Ensifer |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 10 | S → G: Almost no effect. 1 Publication | 1 | |
Mutagenesisi | 13 | A → G: Can use NAD as cosubstrate as well as NADP. 1 Publication | 1 | |
Mutagenesisi | 33 | S → D: No activity. 1 Publication | 1 | |
Mutagenesisi | 94 | K → G: Less than 1% remaining activity. 1 Publication | 1 | |
Mutagenesisi | 176 | D → A: Less than 1% remaining activity. 1 Publication | 1 | |
Mutagenesisi | 180 | H → A: Less than 2% remaining activity. 1 Publication | 1 | |
Mutagenesisi | 206 | G → I: No effect. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000382696 | 1 – 333 | 1,5-anhydro-D-fructose reductaseAdd BLAST | 333 |
Interactioni
Subunit structurei
Monomer.
2 PublicationsStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q2I8V6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q2I8V6 |
Family & Domainsi
Sequence similaritiesi
Belongs to the Gfo/Idh/MocA family.Curated
Family and domain databases
InterProi | View protein in InterPro IPR004104, Gfo/Idh/MocA-like_OxRdtase_C IPR036291, NAD(P)-bd_dom_sf IPR000683, Oxidoreductase_N |
Pfami | View protein in Pfam PF01408, GFO_IDH_MocA, 1 hit PF02894, GFO_IDH_MocA_C, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
i Sequence
Sequence statusi: Complete.
Q2I8V6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNRWGLIGAS TIAREWVIGA IRATGGEVVS MMSTSAERGA AYATENGIGK
60 70 80 90 100
SVTSVEELVG DPDVDAVYVS TTNELHREQT LAAIRAGKHV LCEKPLAMTL
110 120 130 140 150
EDAREMVVAA REAGVVLGTN HHLRNAAAHR AMRDAIAEGR IGRPIAARVF
160 170 180 190 200
HAVYLPPHLQ GWRLERPEAG GGVILDITVH DADTLRFVLN DDPAEAVAIS
210 220 230 240 250
HSAGMGKEGV EDGVMGGVRF QSGVIAQFHD AFTTKFAETG FEVHGTEGSL
260 270 280 290 300
IGRNVMTQKP VGTVTLRNAE GESQLPLDPA NLYETALAAF HSAIEGHGQP
310 320 330
SATGEDGVWS LATGLAVVKA AATGQAAEIE TGL
Mass spectrometryi
Molecular mass is 35100±130 Da. Determined by MALDI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ140417 Genomic DNA Translation: ABA25865.1 |
Genome annotation databases
KEGGi | ag:ABA25865 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ140417 Genomic DNA Translation: ABA25865.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2GLX | X-ray | 2.20 | A/B/C/D/E/F | 2-333 | [»] | |
SMRi | Q2I8V6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Genome annotation databases
KEGGi | ag:ABA25865 |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-16296 |
BRENDAi | 1.1.1.292, 8538 |
SABIO-RKi | Q2I8V6 |
Miscellaneous databases
EvolutionaryTracei | Q2I8V6 |
Family and domain databases
InterProi | View protein in InterPro IPR004104, Gfo/Idh/MocA-like_OxRdtase_C IPR036291, NAD(P)-bd_dom_sf IPR000683, Oxidoreductase_N |
Pfami | View protein in Pfam PF01408, GFO_IDH_MocA, 1 hit PF02894, GFO_IDH_MocA_C, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AFR_ENSAD | |
Accessioni | Q2I8V6Primary (citable) accession number: Q2I8V6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 1, 2009 |
Last sequence update: | March 7, 2006 | |
Last modified: | December 2, 2020 | |
This is version 51 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families