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Protein

Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2A

Gene

inppl1a

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Plays a central role in regulation of PI3K-dependent insulin signaling, although the precise molecular mechanisms and signaling pathways remain unclear. Part of a signaling pathway that regulates actin cytoskeleton remodeling. Required for the maintenance and dynamic remodeling of actin structures as well as in endocytosis, having a major impact on ligand-induced EGFR internalization and degradation. Participates in regulation of cortical and submembraneous actin. Regulates cell adhesion and cell spreading. Acts as a negative regulator of the FC-gamma-RIIA receptor (FCGR2A). Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6 (By similarity).By similarity

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 3,4-diphosphate + phosphate.

GO - Molecular functioni

GO - Biological processi

  • BMP signaling pathway Source: ZFIN
  • cell adhesion Source: UniProtKB-KW
  • immune system process Source: UniProtKB-KW
  • negative regulation of fibroblast growth factor receptor signaling pathway Source: ZFIN
  • negative regulation of neutrophil chemotaxis Source: ZFIN
  • phosphatidylinositol dephosphorylation Source: InterPro

Keywordsi

Molecular functionActin-binding, Hydrolase
Biological processCell adhesion, Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2A (EC:3.1.3.86)
Alternative name(s):
Inositol polyphosphate phosphatase-like protein 1A
Short name:
INPPL1-A
SH2 domain-containing inositol 5'-phosphatase 2A
Short name:
SH2 domain-containing inositol phosphatase 2A
Short name:
SHIP-2A
Gene namesi
Name:inppl1a
Synonyms:ship2a
ORF Names:wu:fc58a04, zgc:198309
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-030131-3904 inppl1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003028731 – 1266Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2AAdd BLAST1266

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei961PhosphotyrosineBy similarity1

Post-translational modificationi

Tyrosine phosphorylated by the members of the SRC family after exposure to a diverse array of extracellular stimuli.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ2I6J1

Interactioni

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ2I6J1
SMRiQ2I6J1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 111SH2PROSITE-ProRule annotationAdd BLAST97
Domaini1203 – 1266SAMPROSITE-ProRule annotationAdd BLAST64

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi958 – 961NPXY motif4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi901 – 905Poly-Ser5
Compositional biasi1046 – 1121Pro-richAdd BLAST76

Domaini

The SH2 domain interacts with tyrosine phosphorylated forms of proteins.By similarity
The NPXY sequence motif found in many tyrosine-phosphorylated proteins is required for the specific binding of the PID domain.By similarity

Sequence similaritiesi

Keywords - Domaini

SH2 domain

Phylogenomic databases

HOGENOMiHOG000004836
HOVERGENiHBG106726
InParanoidiQ2I6J1
KOiK15909
PhylomeDBiQ2I6J1

Family and domain databases

Gene3Di3.30.505.10, 1 hit
3.60.10.10, 1 hit
InterProiView protein in InterPro
IPR036691 Endo/exonu/phosph_ase_sf
IPR000300 IPPc
IPR001660 SAM
IPR013761 SAM/pointed_sf
IPR000980 SH2
IPR036860 SH2_dom_sf
PfamiView protein in Pfam
PF00536 SAM_1, 1 hit
PF00017 SH2, 1 hit
PRINTSiPR00401 SH2DOMAIN
SMARTiView protein in SMART
SM00128 IPPc, 1 hit
SM00454 SAM, 1 hit
SM00252 SH2, 1 hit
SUPFAMiSSF47769 SSF47769, 1 hit
SSF55550 SSF55550, 1 hit
SSF56219 SSF56219, 1 hit
PROSITEiView protein in PROSITE
PS50105 SAM_DOMAIN, 1 hit
PS50001 SH2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q2I6J1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAVGPASPP PPPLWMHRDL SRAAAEELLA RAGRDGSFLV RDSESVSGAY
60 70 80 90 100
ALCVLFQKHV HTYRILPDEE NFLAVQTSQG VQPKRFKTLP ELIQLYLQPS
110 120 130 140 150
QGLVTTLLYP VEREETTEDR DYSDGEDEKP PLPPRTASTS SMTGSALVST
160 170 180 190 200
DTPPENVTAA NGLSTISHEY LKGNYALDLE AVKQGANSLP HLNKTLLSSC
210 220 230 240 250
KRLHGEVDKV LCGLEILSKV FDQQSASMVS RMIQQSMSQG GDQELEHLVT
260 270 280 290 300
KLAILKDLLS SIEKKALKAL QDMSSSTPAI SPLLSIRNKV IPVQTFEVKL
310 320 330 340 350
DVYLADLTKI GKSQKYSLSV DVEGGKLVVM KKMKDAQEDW NTFTHDKIRQ
360 370 380 390 400
LIKSQRVQNK LGIVFEKEKD KSQRKDFIFA SAKKREAFCQ LLQLMKNKHS
410 420 430 440 450
NQDEPDMISI FIGTWNMGSV PAPKPLGSWI LSRGLGKTLD EMAVTIPHDI
460 470 480 490 500
YVFGTQENSV CDKEWVETLR CSLKEYTDME YKPIAVQTLW NIKIVVLVKA
510 520 530 540 550
EHENRISHVG MSSVKTGIAN TLGNKGAVGV SFMFNGTSFG FVNCHLTSGN
560 570 580 590 600
EKIHRRNQNY LDILRQLSLG DKQLNSFDIS LRFTHLFWFG DLNYRLDMDI
610 620 630 640 650
QEILNYINRK EFDPLLKVDQ LNLEREKNKI FLRFAEEEIS YPPTYRYERG
660 670 680 690 700
SRDTYVWQKQ KATGMRTNVP SWCDRILWKS YPETHIVCNS YGCTDDIVTS
710 720 730 740 750
DHSPVFGTFE VGVTSQFVSK KGLPKSSEQA YIEFENIEAI VKTASRTKFF
760 770 780 790 800
IEFYSTCLEE FKKSYENDTQ SSDNVNFLRV GWSSKQLTTL KPILSDIEYL
810 820 830 840 850
QDQHLLLTVK SLDGYESYGE CVLALKSMIG STAQQFHTYL SHRGEETGNI
860 870 880 890 900
RGSMRVRVPS ERMGTRERLY EWISVDKDDM GGPKGRTLPP RTSHDYVKPT
910 920 930 940 950
SSSSSRKHGS AELSRVSEEG EKSSTSRHTH TKEENTHNRG KQDPFESDAT
960 970 980 990 1000
TCKNSFNNPA YYILEGVPNQ SAALASDILP GSALPPLANK TTAPPAGSVG
1010 1020 1030 1040 1050
KSKPPSGSSA QGRWQTSGRS VRPISEEGSS EDDGNIGPHT GSLNRPPPDF
1060 1070 1080 1090 1100
PPPPLPKAAL EMSENSFGKP RVFSDLADGK IPPPSKPLVS PGLTPPPGGA
1110 1120 1130 1140 1150
PGGGVAFCIE SPPVLSPNSA PFRRGGGASA LDDQSCSVLQ MAKTLSEVEY
1160 1170 1180 1190 1200
PSGRERGASQ GPTGPQLRGL SFPSHPIQEE SIAEDLPEEG GLWGAESSSS
1210 1220 1230 1240 1250
SLSVDCSVGE WLQKLGLQHY EEGLLHNGWD DLEFLSDITE EDLEEAGVRD
1260
PAHKKILLAS LKQQQK
Length:1,266
Mass (Da):140,371
Last modified:March 23, 2010 - v2
Checksum:i46AC69D19E22225B
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4ISQ5A0A0R4ISQ5_DANRE
Phosphatidylinositol 3,4,5-trisphos...
inppl1a
785Annotation score:
A0A0R4IB77A0A0R4IB77_DANRE
Phosphatidylinositol 3,4,5-trisphos...
inppl1a
1,003Annotation score:
A0A0J9YJ69A0A0J9YJ69_DANRE
Phosphatidylinositol 3,4,5-trisphos...
inppl1a
260Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23A → T in ABB84851 (Ref. 1) Curated1
Sequence conflicti132L → P in ABB84851 (Ref. 1) Curated1
Sequence conflicti929T → P in ABB84851 (Ref. 1) Curated1
Sequence conflicti1037G → V in ABB84851 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ272661 mRNA Translation: ABB84851.1
BC171582 mRNA Translation: AAI71582.1
RefSeqiNP_001034893.1, NM_001039804.2
UniGeneiDr.155386

Genome annotation databases

GeneIDi325179
KEGGidre:325179

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ272661 mRNA Translation: ABB84851.1
BC171582 mRNA Translation: AAI71582.1
RefSeqiNP_001034893.1, NM_001039804.2
UniGeneiDr.155386

3D structure databases

ProteinModelPortaliQ2I6J1
SMRiQ2I6J1
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ2I6J1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi325179
KEGGidre:325179

Organism-specific databases

CTDi325179
ZFINiZDB-GENE-030131-3904 inppl1a

Phylogenomic databases

HOGENOMiHOG000004836
HOVERGENiHBG106726
InParanoidiQ2I6J1
KOiK15909
PhylomeDBiQ2I6J1

Miscellaneous databases

PROiPR:Q2I6J1

Family and domain databases

Gene3Di3.30.505.10, 1 hit
3.60.10.10, 1 hit
InterProiView protein in InterPro
IPR036691 Endo/exonu/phosph_ase_sf
IPR000300 IPPc
IPR001660 SAM
IPR013761 SAM/pointed_sf
IPR000980 SH2
IPR036860 SH2_dom_sf
PfamiView protein in Pfam
PF00536 SAM_1, 1 hit
PF00017 SH2, 1 hit
PRINTSiPR00401 SH2DOMAIN
SMARTiView protein in SMART
SM00128 IPPc, 1 hit
SM00454 SAM, 1 hit
SM00252 SH2, 1 hit
SUPFAMiSSF47769 SSF47769, 1 hit
SSF55550 SSF55550, 1 hit
SSF56219 SSF56219, 1 hit
PROSITEiView protein in PROSITE
PS50105 SAM_DOMAIN, 1 hit
PS50001 SH2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSHP2A_DANRE
AccessioniPrimary (citable) accession number: Q2I6J1
Secondary accession number(s): B7ZVH1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 23, 2010
Last modified: November 22, 2017
This is version 76 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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