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Protein

Acidic phospholipase A2 BE-I-PLA2

Gene
N/A
Organism
Bothrops erythromelas (Caatinga lance head)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Snake venom phospholipase A2 that shows a potent inhibition of human platelet aggregation. This inhibition is concentration-dependent when aggregation is induced by collagen, and concentration-independent when aggregation is induced by arachidonic acid. In human umbilical-cord vein endothelial cells, this toxin stimulates endothelial cells to release prostaglandin I2, suggesting an increase of its potential anti-platelet activity in vivo. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.1 Publication

Miscellaneous

Does not inhibit platelet aggregation when aggregation is induced by ADP, and does not induce aggregation of washed platelets. Does not induce changes on the principal platelet receptors (ITGA2, ITGA2B, GPIBA, SELP). In human umbilical-cord vein endothelial cells, does not induce detachment, does not interfere on nitric oxide release, and is neither apoptotic nor proliferative (PubMed:16750518).1 Publication

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi45Calcium; via carbonyl oxygenBy similarity1
Metal bindingi47Calcium; via carbonyl oxygenBy similarity1
Active sitei63By similarity1
Metal bindingi64CalciumBy similarity1
Active sitei105By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHemostasis impairing toxin, Hydrolase, Platelet aggregation inhibiting toxin, Toxin
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Acidic phospholipase A2 BE-I-PLA2 (EC:3.1.1.4)
Short name:
svPLA2
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
OrganismiBothrops erythromelas (Caatinga lance head)
Taxonomic identifieri44710 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeBothrops

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 161 PublicationAdd BLAST16
ChainiPRO_500014119117 – 138Acidic phospholipase A2 BE-I-PLA2Add BLAST122

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi42 ↔ 131By similarity
Disulfide bondi44 ↔ 60By similarity
Disulfide bondi59 ↔ 111By similarity
Disulfide bondi65 ↔ 138By similarity
Disulfide bondi66 ↔ 104By similarity
Disulfide bondi73 ↔ 97By similarity
Disulfide bondi91 ↔ 102By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ2HZ28

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

3D structure databases

ProteinModelPortaliQ2HZ28
SMRiQ2HZ28
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG008137

Family and domain databases

CDDicd00125 PLA2c, 1 hit
Gene3Di1.20.90.10, 1 hit
InterProiView protein in InterPro
IPR001211 PLipase_A2
IPR033112 PLipase_A2_Asp_AS
IPR016090 PLipase_A2_dom
IPR036444 PLipase_A2_dom_sf
IPR033113 PLipase_A2_His_AS
PANTHERiPTHR11716 PTHR11716, 1 hit
PfamiView protein in Pfam
PF00068 Phospholip_A2_1, 1 hit
PRINTSiPR00389 PHPHLIPASEA2
SMARTiView protein in SMART
SM00085 PA2c, 1 hit
SUPFAMiSSF48619 SSF48619, 1 hit
PROSITEiView protein in PROSITE
PS00119 PA2_ASP, 1 hit
PS00118 PA2_HIS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2HZ28-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRTLWIMAVL LVGVEGSLVQ FETLIMKIAG RSGVWYYGSY GCYCGSGGQG
60 70 80 90 100
RPQDASDRCC FVHDCCYGKV TDCDPKADVY TYSEENGVVV CGGDDPCKKQ
110 120 130
ICECDRVAAT CFRDNKDTYD NKYWFFPAKN CQEESEPC
Length:138
Mass (Da):15,435
Last modified:March 7, 2006 - v1
Checksum:i760A7A10AE6943C8
GO

Mass spectrometryi

Molecular mass is 13649.57 Da from positions 17 - 138. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ359953 mRNA Translation: ABC96692.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ359953 mRNA Translation: ABC96692.1

3D structure databases

ProteinModelPortaliQ2HZ28
SMRiQ2HZ28
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ2HZ28

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG008137

Family and domain databases

CDDicd00125 PLA2c, 1 hit
Gene3Di1.20.90.10, 1 hit
InterProiView protein in InterPro
IPR001211 PLipase_A2
IPR033112 PLipase_A2_Asp_AS
IPR016090 PLipase_A2_dom
IPR036444 PLipase_A2_dom_sf
IPR033113 PLipase_A2_His_AS
PANTHERiPTHR11716 PTHR11716, 1 hit
PfamiView protein in Pfam
PF00068 Phospholip_A2_1, 1 hit
PRINTSiPR00389 PHPHLIPASEA2
SMARTiView protein in SMART
SM00085 PA2c, 1 hit
SUPFAMiSSF48619 SSF48619, 1 hit
PROSITEiView protein in PROSITE
PS00119 PA2_ASP, 1 hit
PS00118 PA2_HIS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPA2A_BOTER
AccessioniPrimary (citable) accession number: Q2HZ28
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: March 7, 2006
Last modified: May 23, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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